cDNA Clone LAS1L

• Synonyms: ribosomal biogenesis protein LAS1L
• Plasmid: pGEX6P HALO Thr-LAS1L isoform 2
• Parent Plasmid: pGEX 3C HALO Thr
• Expressed: GST-HALO-LAS1L isoform 2
• Genbank: NP_112483.1 (uni: Q9Y4W2-1)
• Tag: GST
• Tag: HALO
• Species: Human

Sequence of Insert

agatctATGTCGTGGGAATCCGGGGCCGGGCCAGGTCTAGGTTCCCAGGG
GATGGATCTCGTGTGGAGTGCGTGGTACGGAAAGTGCGTTAAAGGGAAAG
GGTCGTTGCCACTCTCGGCCCACGGCATCGTGGTCGCCTGGCTCAGCAGG
GCCGAGTGGGACCAGGTGACGGTTTATCTGTTCTGTGACGACCATAAGTT
GCAGCGGTACGCGCTTAACCGCATCACGGTGTGGAGGAGCAGGTCAGGCA
ACGAACTCCCTCTGGCAGTGGCTTCTACTGCTGACCTGATACGCTGTAAG
CTCTTGGATGTAACTGGTGGCTTGGGCACTGATGAACTTAGACTGCTCTA
TGGCATGGCATTGGTCAGGTTTGTGAATCTTATCTCAGAGAGGAAGACAA
AGTTTGCCAAGGTCCCCCTCAAGTGTCTGGCTCAAGAGGTAAATATTCCG
GATTGGATTGTTGACCTTCGCCATGAGTTGACCCACAAGAAAATGCCCCA
TATAAATGACTGCCGCAGAGGCTGCTACTTTGTCCTGGATTGGCTCCAGA
AGACCTATTGGTGCCGCCAACTGGAGAACAGCCTGAGAGAGACCTGGGAG
TTGGAGGAGTTCAGGGAAGGGATAGAGGAAGAGGATCAAGAGGAAGATAA
GAACATTGTTGTTGATGACATCACAGAACAGAAACCAGAGCCTCAGGATG
ATGGGAAAAGTACGGAGTCAGATGTAAAGGCCGATGGAGACAGCAAAGGC
AGCGAAGAGGTGGATTCTCATTGCAAAAAGGCCCTGAGTCATAAAGAGCT
ATATGAAAGAGCCCGAGAACTGCTGGTATCATACGAAGAGGAGCAGTTTA
CGGTGCTGGAGAAATTTAGGTATTTACCTAAGGCCATTAAGGCGTGGAAT
AACCCGTCCCCACGTGTAGAATGTGTCCTGGCAGAGCTCAAGGGCGTTAC
ATGCGAGAACAGGGAGGCTGTGCTGGATGCTTTTCTGGATGATGGCTTCC
TTGTCCCCACATTTGAACAGTTGGCAGCTTTGCAGATAGAATATGAAGAA
AACGTGGACTTGAATGACGTCCTGGTGCCAAAGCCGTTCTCTCAGTTCTG
GCAGCCCCTGCTCAGGGGCCTGCACTCCCAGAACTTCACGCAGGCCCTAT
TGGAGAGGATGCTCTCTGAACTGCCAGCCTTGGGGATCAGCGGGATCCGG
CCTACCTACATCCTCAGATGGACCGTTGAACTGATCGTGGCCAACACCAA
GACTGGACGGAATGCTCGCCGATTTTCTGCAGGCCAGTGGGAAGCAAGAA
GGGGCTGGAGGCTGTTCAACTGCTCCGCCTCCCTTGACTGGCCCCGGATG
GTTGAGTCCTGCTTGGGCTCACCTTGCTGGGCCAGCCCCCAACTCCTTCG
GATCATCTTCAAAGCCATGGGGCAGGGCCTGCCAGACGAGGAGCAGGAGA
AGCTGCTGCGCATCTGTTCCATTTATACCCAGAGTGGAGAAAACAGCCTG
GTGCAGGAGGGCTCTGAGGCCTCCCCCATTGGGAAGTCTCCATATACACT
AGACAGCCTGTATTGGAGCGTCAAGCCAGCCAGCTCCAGCTTCGGGTCTG
AAGCAAAGGCCCAGCAACAGGAGGAGCAGGGCAGTGTTAATGATGTCAAG
GAAGAGGAGAAGGAGGAGAAAGAGGTCTTGCCAGACCAGGTAGAGGAGGA
GGAAGAAAATGATGACCAAGAGGAGGAAGAGGAGGATGAAGATGATGAAG
ATGATGAAGAGGAAGACAGAATGGAGGTGGGGCCTTTCTCTACAGGGCAA
GAGTCCCCCACTGCCGAGAATGCTAGGCTTCTGGCCCAGAAAAGAGGAGC
TTTGCAGGGCTCTGCATGGCAGGTTAGCTCAGAAGACGTGCGATGGGACA
CATTTCCCCTAGGCCGAATGCCAGGTCAGACCGAGGACCCAGCAGAGCTC
ATGCTGGAGAATTATGACACCATGTATCTTTTGGACCAGCCTGTGCTAGA
GCAGCGGCTGGAACCCTCAACATGCAAGACTGACACCTTGGGCCTGAGCT
GTGGTGTCGGCAGTGGCAACTGCAGCAACAGCAGCAGCAGCAACTTCGAG
GGCCTTCTCTGGAGCCAGGGGCAGCTGCATGGGCTCAAAACTGGCCTGCA
GCTCTTCTGAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGLVPRGSGSMSWESGAGPGLGSQ
GMDLVWSAWYGKCVKGKGSLPLSAHGIVVAWLSRAEWDQVTVYLFCDDHK
LQRYALNRITVWRSRSGNELPLAVASTADLIRCKLLDVTGGLGTDELRLL
YGMALVRFVNLISERKTKFAKVPLKCLAQEVNIPDWIVDLRHELTHKKMP
HINDCRRGCYFVLDWLQKTYWCRQLENSLRETWELEEFREGIEEEDQEED
KNIVVDDITEQKPEPQDDGKSTESDVKADGDSKGSEEVDSHCKKALSHKE
LYERARELLVSYEEEQFTVLEKFRYLPKAIKAWNNPSPRVECVLAELKGV
TCENREAVLDAFLDDGFLVPTFEQLAALQIEYEENVDLNDVLVPKPFSQF
WQPLLRGLHSQNFTQALLERMLSELPALGISGIRPTYILRWTVELIVANT
KTGRNARRFSAGQWEARRGWRLFNCSASLDWPRMVESCLGSPCWASPQLL
RIIFKAMGQGLPDEEQEKLLRICSIYTQSGENSLVQEGSEASPIGKSPYT
LDSLYWSVKPASSSFGSEAKAQQQEEQGSVNDVKEEEKEEKEVLPDQVEE
EEENDDQEEEEEDEDDEDDEEEDRMEVGPFSTGQESPTAENARLLAQKRG
ALQGSAWQVSSEDVRWDTFPLGRMPGQTEDPAELMLENYDTMYLLDQPVL
EQRLEPSTCKTDTLGLSCGVGSGNCSNSSSSNFEGLLWSQGQLHGLKTGL
QLF*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU28600
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: This isoform contains D348E mutation plus deletion spanning Gly349-Lys365 compared to NCBI ref (isoform 1)
• Low Temp: No