cDNA Clone HECTD1

• Plasmid: pGEX C3 HALO thr-HECTD1 Met2149-2610 (END)
• Parent Plasmid: pGEX C3 HALO thr
• Expressed: GST-HALO-HECTD1 Met2149-2610 (END)
• Genbank: Q9ULT8-1 (No genbank)
• Tag: GST
• Tag: HALO
• Species: Human

Sequence of Insert

ggatccATGCAAATACATGCAGATCGGAAATCAGTTCTTGAGGTTGAATT
TTTAGGAGAAGAAGGAACTGGCTTGGGACCCACATTAGAGTTTTATGCTC
TGGTGGCAGCAGAATTCCAGAGAACTGACTTGGGAGCTTGGCTTTGTGAT
GATAATTTTCCAGATGATGAATCTCGTCACGTTGATCTTGGAGGTGGATT
GAAACCTCCTGGATATTATGTGCAGAGGTCATGTGGACTGTTCACAGCAC
CATTTCCACAGGATAGTGATGAGCTTGAAAGGATCACGAAACTGTTTCAT
TTCCTTGGAATTTTCTTGGCCAAATGCATTCAAGACAATAGACTTGTGGA
CTTACCTATTTCTAAACCTTTTTTTAAACTTATGTGTATGGGTGACATTA
AAAGCAATATGAGTAAACTGATTTATGAGTCACGAGGTGATAGAGACTTA
CACTGTACTGAAAGTCAGTCTGAAGCTTCTACAGAAGAAGGTCATGATTC
ACTCTCGGTAGGAAGCTTTGAAGAGGATTCAAAATCAGAATTTATTCTTG
ATCCCCCTAAACCAAAACCCCCAGCTTGGTTTAATGGAATTTTGACTTGG
GAAGACTTTGAATTAGTAAACCCACACAGAGCCAGATTTTTAAAAGAAAT
TAAAGACCTTGCTATCAAGAGGCGCCAAATTTTAAGCAACAAAGGTCTTT
CTGAAGATGAGAAGAACACAAAATTACAGGAACTAGTGCTGAAGAATCCA
TCAGGTTCTGGGCCTCCACTTAGCATAGAGGATTTAGGTTTAAATTTCCA
GTTTTGCCCTTCCTCAAGAATATATGGTTTTACAGCTGTGGATCTCAAGC
CAAGTGGTGAAGATGAGATGATAACAATGGATAATGCAGAAGAATATGTG
GATTTGATGTTTGACTTTTGTATGCATACGGGTATTCAGAAACAAATGGA
AGCCTTTAGAGATGGGTTTAATAAAGTTTTTCCAATGGAGAAATTAAGTT
CCTTCAGCCATGAAGAAGTCCAAATGATTCTTTGTGGAAACCAGTCACCA
TCCTGGGCAGCAGAGGATATTATCAATTACACTGAACCTAAGCTGGGTTA
TACACGTGACAGCCCTGGTTTCCTGAGGTTTGTGAGGGTTTTATGTGGCA
TGTCTTCTGATGAAAGGAAAGCATTCTTGCAGTTTACCACTGGTTGTTCA
ACTCTACCCCCAGGTGGACTGGCTAACCTGCATCCCAGGCTCACGGTTGT
ACGCAAGGTTGATGCTACTGATGCAAGCTATCCATCAGTCAATACATGTG
TGCATTACCTTAAGTTGCCTGAATATTCTTCCGAGGAGATCATGAGAGAG
CGCCTGCTAGCTGCTACAATGGAGAAAGGCTTTCATCTCAATTGAgcggc
cgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGLVPRGSGSMQIHADRKSVLEVE
FLGEEGTGLGPTLEFYALVAAEFQRTDLGAWLCDDNFPDDESRHVDLGGG
LKPPGYYVQRSCGLFTAPFPQDSDELERITKLFHFLGIFLAKCIQDNRLV
DLPISKPFFKLMCMGDIKSNMSKLIYESRGDRDLHCTESQSEASTEEGHD
SLSVGSFEEDSKSEFILDPPKPKPPAWFNGILTWEDFELVNPHRARFLKE
IKDLAIKRRQILSNKGLSEDEKNTKLQELVLKNPSGSGPPLSIEDLGLNF
QFCPSSRIYGFTAVDLKPSGEDEMITMDNAEEYVDLMFDFCMHTGIQKQM
EAFRDGFNKVFPMEKLSSFSHEEVQMILCGNQSPSWAAEDIINYTEPKLG
YTRDSPGFLRFVRVLCGMSSDERKAFLQFTTGCSTLPPGGLANLHPRLTV
VRKVDATDASYPSVNTCVHYLKLPEYSSEEIMRERLLAATMEKGFHLN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU28626
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Grow at or below 30ºC
• Low Temp: Yes