cDNA Clone USP1 (human)/USP2 (rat)

• Synonyms: UBP
• Plasmid: pGEX6P1 human USP1 M1-V60 rat USP2 M271-M618(end) C289A
• Parent Plasmid: pGEX6P1
• Expressed: GST 3C human USP1 M1-V60 rat USP2 M271-M618(end) C289A
• Genbank: BC085719.1 (USP2) NM_001017415.1 (USP1)
• Tag: GST

Sequence of Insert

GGATCCATGCCTGGTGTCATACCTAGTGAAAGTAATGGACTTTCAAGAGG
TAGCCCTTCAAAGAAAAACAGACTTTCCTTAAAGTTTTTTCAGAAAAAGG
AAACTAAGAGAGCTTTGGATTTCACAGATTCTCAAGAAAATGAAGAAAAA
GCTTCTGAATATAGAGCATCTGAAATTGATCAAGTTGGATCCATGAATTC
AAAGAGTGCCCAGGGTCTGGCTGGTCTTCGAAACCTTGGGAACACGGCCT
TCATGAACTCAATTCTTCAGTGTCTGAGCAACACCCGCGAGTTGAGAGAT
TACTGCCTCCAGAGGCTGTACATGCGGGACCTCGGCCATACCAGCAGTGC
ACACACAGCTCTCATGGAAGAGTTTGCAAAACTAATCCAGACCATATGGA
CGTCATCCCCCAATGATGTGGTGAGCCCATCTGAGTTCAAGACCCAGATC
CAGAGATATGCACCACGCTTCGTTGGCTATAATCAGCAGGATGCTCAGGA
ATTCCTCCGTTTCCTTCTGGATGGCCTCCACAACGAGGTGAACCGGGTGG
CAGCAAGGCCTAAGCCCAGCCCTGAGAGCCTTGATCATCTCCCTGATGAA
GAGAAAGGGCGACAGATGTGGAGGAAGTATCTAGAAAGGGAAGACAGTCG
GATTGGGGATCTCTTCGTTGGGCAGCTAAAGAGCTCCCTGACGTGCACCG
ACTGCGGCTACTGCTCTACAGTGTTCGATCCCTTCTGGGACCTCTCGTTG
CCCATTGCAAAGAGAGGCTATCCTGAAGTGACGTTAATGGATTGTATGAG
GCTCTTCACCAAAGAGGATGTGTTGGATGGGGATGAGAAACCAACATGCT
GCCGCTGCCGGGCCAGAAAACGATGTATAAAGAAGTTCTCTGTCCAGAGG
TTCCCAAAGATCTTGGTGCTCCACCTGAAGCGGTTCTCAGAATCCAGGAT
ACGAACCAGCAAGCTCACAACATTTGTGAATTTCCCACTAAGAGACCTGG
ACTTGAGAGAATTTGCTTCAGAAAACACCAACCATGCTGTTTACAACCTG
TATGCTGTGTCCAATCACTCCGGAACCACCATGGGAGGTCACTATACAGC
CTACTGCCGAAGTCCGGTTACGGGCGAATGGCACACTTTCAATGACTCCA
GTGTCACACCCATGTCCTCCAGCCAAGTGCGCACCAGCGACGCCTATTTG
CTCTTCTATGAACTGGCCAGTCCACCCTCCCGTATGTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMPGVIPSESNGLSRGSPSK
KNRLSLKFFQKKETKRALDFTDSQENEEKASEYRASEIDQVGSMNSKSAQ
GLAGLRNLGNTAFMNSILQCLSNTRELRDYCLQRLYMRDLGHTSSAHTAL
MEEFAKLIQTIWTSSPNDVVSPSEFKTQIQRYAPRFVGYNQQDAQEFLRF
LLDGLHNEVNRVAARPKPSPESLDHLPDEEKGRQMWRKYLEREDSRIGDL
FVGQLKSSLTCTDCGYCSTVFDPFWDLSLPIAKRGYPEVTLMDCMRLFTK
EDVLDGDEKPTCCRCRARKRCIKKFSVQRFPKILVLHLKRFSESRIRTSK
LTTFVNFPLRDLDLREFASENTNHAVYNLYAVSNHSGTTMGGHYTAYCRS
PVTGEWHTFNDSSVTPMSSSQVRTSDAYLLFYELASPPSRM*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU28829
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: C289A rUSP2
• Low Temp: No