cDNA Clone TSC2

• Plasmid: pcDNA5D FRT/TO-TSC2-C-FLAG Isoform 1
• Parent Plasmid: pcDNA5D FRT/TO-C-FLAG
• Expressed: TSC2-FLAG
• Genbank: NM_000548.3
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGGCCAAACCAACAAGCAAAGATTCAGGCTTGAAGGAGAAGTT
TAAGATTCTGTTGGGACTGGGAACACCGAGGCCAAATCCCAGGTCTGCAG
AGGGTAAACAGACGGAGTTTATCATCACCGCGGAAATACTGAGAGAACTG
AGCATGGAATGTGGCCTCAACAATCGCATCCGGATGATAGGGCAGATTTG
TGAAGTCGCAAAAACCAAGAAATTTGAAGAGCACGCAGTGGAAGCACTCT
GGAAGGCGGTCGCGGATCTGTTGCAGCCGGAGCGGCCGCTGGAGGCCCGG
CACGCGGTGCTGGCTCTGCTGAAGGCCATCGTGCAGGGGCAGGGCGAGCG
TTTGGGGGTCCTCAGAGCCCTCTTCTTTAAGGTCATCAAGGATTACCCTT
CCAACGAAGACCTTCACGAAAGGCTGGAGGTTTTCAAGGCCCTCACAGAC
AATGGGAGACACATCACCTACTTGGAGGAAGAGCTGGCTGACTTTGTCCT
GCAGTGGATGGATGTTGGCTTGTCCTCGGAATTCCTTCTGGTGCTGGTGA
ACTTGGTCAAATTCAATAGCTGTTACCTCGACGAGTACATCGCAAGGATG
GTTCAGATGATCTGTCTGCTGTGCGTCCGGACCGCGTCCTCTGTGGACAT
AGAGGTCTCCCTGCAGGTGCTGGACGCCGTGGTCTGCTACAACTGCCTGC
CGGCTGAGAGCCTCCCGCTGTTCATCGTTACCCTCTGTCGCACCATCAAC
GTCAAGGAGCTCTGCGAGCCTTGCTGGAAGCTGATGCGGAACCTCCTTGG
CACCCACCTGGGCCACAGCGCCATCTACAACATGTGCCACCTCATGGAGG
ACAGAGCCTACATGGAGGACGCGCCCCTGCTGAGAGGAGCCGTGTTTTTT
GTGGGCATGGCTCTCTGGGGAGCCCACCGGCTCTATTCTCTCAGGAACTC
GCCGACATCTGTGTTGCCATCATTTTACCAGGCCATGGCATGTCCGAACG
AGGTGGTGTCCTATGAGATCGTCCTGTCCATCACCAGGCTCATCAAGAAG
TATAGGAAGGAGCTCCAGGTGGTGGCGTGGGACATTCTGCTGAACATCAT
CGAACGGCTCCTTCAGCAGCTCCAGACCTTGGACAGCCCGGAGCTCAGGA
CCATCGTCCATGACCTGTTGACCACGGTGGAGGAGCTGTGTGACCAGAAC
GAGTTCCACGGGTCTCAGGAGAGATACTTTGAACTGGTGGAGAGATGTGC
GGACCAGAGGCCTGAGTCCTCCCTCCTGAACCTGATCTCCTATAGAGCGC
AGTCCATCCACCCGGCCAAGGACGGCTGGATTCAGAACCTGCAGGCGCTG
ATGGAGAGATTCTTCAGGAGCGAGTCCCGAGGCGCCGTGCGCATCAAGGT
GCTGGACGTGCTGTCCTTTGTGCTGCTCATCAACAGGCAGTTCTATGAGG
AGGAGCTGATTAACTCAGTGGTCATCTCGCAGCTCTCCCACATCCCCGAG
GATAAAGACCACCAGGTCCGAAAGCTGGCCACCCAGTTGCTGGTGGACCT
GGCAGAGGGCTGCCACACACACCACTTCAACAGCCTGCTGGACATCATCG
AGAAGGTGATGGCCCGCTCCCTCTCCCCACCCCCGGAGCTGGAAGAAAGG
GATGTGGCCGCATACTCGGCCTCCTTGGAGGATGTGAAGACAGCCGTCCT
GGGGCTTCTGGTCATCCTTCAGACCAAGTTGTACACCCTGCCTGCAAGCC
ACGCCACGCGTGTGTATGAGATGCTGGTCAGCCACATTCAGCTCCACTAC
AAGCACAGCTACACCCTGCCAATCGCGAGCAGCATCCGGCTGCAGGCCTT
TGACTTCCTGTTGCTGCTGCGGGCCGACTCACTGCACCGCCTGGGCCTGC
CCAACAAGGATGGAGTCGTGCGGTTCAGCCCCTACTGCGTCTGCGACTAC
ATGGAGCCAGAGAGAGGCTCTGAGAAGAAGACCAGCGGCCCCCTTTCTCC
TCCCACAGGGCCTCCTGGCCCGGCGCCTGCAGGCCCCGCCGTGCGGCTGG
GGTCCGTGCCCTACTCCCTGCTCTTCCGCGTCCTGCTGCAGTGCTTGAAG
CAGGAGTCTGACTGGAAGGTGCTGAAGCTGGTTCTGGGCAGGCTGCCTGA
GTCCCTGCGCTATAAAGTGCTCATCTTTACTTCCCCTTGCAGTGTGGACC
AGCTGTGCTCTGCTCTCTGCTCCATGCTTTCAGGCCCAAAGACACTGGAG
CGGCTCCGAGGCGCCCCAGAAGGCTTCTCCAGAACTGACTTGCACCTGGC
CGTGGTTCCAGTGCTGACAGCATTAATCTCTTACCATAACTACCTGGACA
AAACCAAACAGCGCGAGATGGTCTACTGCCTGGAGCAGGGCCTCATCCAC
CGCTGTGCCAGCCAGTGCGTCGTGGCCTTGTCCATCTGCAGCGTGGAGAT
GCCTGACATCATCATCAAGGCGCTGCCTGTTCTGGTGGTGAAGCTCACGC
ACATCTCAGCCACAGCCAGCATGGCCGTCCCACTGCTGGAGTTCCTGTCC
ACTCTGGCCAGGCTGCCGCACCTCTACAGGAACTTTGCCGCGGAGCAGTA
TGCCAGTGTGTTCGCCATCTCCCTGCCGTACACCAACCCCTCCAAGTTTA
ATCAGTACATCGTGTGTCTGGCCCATCACGTCATAGCCATGTGGTTCATC
AGGTGCCGCCTGCCCTTCCGGAAGGATTTTGTCCCTTTCATCACTAAGGG
CCTGCGGTCCAATGTCCTCTTGTCTTTTGATGACACCCCCGAGAAGGACA
GCTTCAGGGCCCGGAGTACTAGTCTCAACGAGAGACCCAAGAGTCTGAGG
ATAGCCAGACCCCCCAAACAAGGCTTGAATAACTCTCCACCCGTGAAAGA
ATTCAAGGAGAGCTCTGCAGCCGAGGCCTTCCGGTGCCGCAGCATCAGTG
TGTCTGAACATGTGGTCCGCAGCAGGATACAGACGTCCCTCACCAGTGCC
AGCTTGGGGTCTGCAGATGAGAACTCCGTGGCCCAGGCTGACGATAGCCT
GAAAAACCTCCACCTGGAGCTCACGGAAACCTGTCTGGACATGATGGCTC
GATACGTCTTCTCCAACTTCACGGCTGTCCCGAAGAGGTCTCCTGTGGGC
GAGTTCCTCCTAGCGGGTGGCAGGACCAAAACCTGGCTGGTTGGGAACAA
GCTTGTCACTGTGACGACAAGCGTGGGAACCGGGACCCGGTCGTTACTAG
GCCTGGACTCGGGGGAGCTGCAGTCCGGCCCGGAGTCGAGCTCCAGCCCC
GGGGTGCATGTAAGACAGACCAAGGAGGCGCCGGCCAAGCTGGAGTCCCA
GGCTGGGCAGCAGGTGTCCCGTGGGGCCCGGGATCGGGTCCGTTCCATGT
CGGGGGGCCATGGTCTTCGAGTTGGCGCCCTGGACGTGCCGGCCTCCCAG
TTCCTGGGCAGTGCCACTTCTCCAGGACCACGGACTGCACCAGCCGCGAA
ACCTGAGAAGGCCTCAGCTGGCACCCGGGTTCCTGTGCAGGAGAAGACGA
ACCTGGCGGCCTATGTGCCCCTGCTGACCCAGGGCTGGGCGGAGATCCTG
GTCCGGAGGCCCACAGGGAACACCAGCTGGCTGATGAGCCTGGAGAACCC
GCTCAGCCCTTTCTCCTCGGACATCAACAACATGCCCCTGCAGGAGCTGT
CTAACGCCCTCATGGCGGCTGAGCGCTTCAAGGAGCACCGGGACACAGCC
CTGTACAAGTCACTGTCGGTGCCGGCAGCCAGCACGGCCAAACCCCCTCC
TCTGCCTCGCTCCAACACAGTGGCCTCTTTCTCCTCCCTGTACCAGTCCA
GCTGCCAAGGACAGCTGCACAGGAGCGTTTCCTGGGCAGACTCCGCCGTG
GTCATGGAGGAGGGAAGTCCGGGCGAGGTTCCTGTGCTGGTGGAGCCCCC
AGGGTTGGAGGACGTTGAGGCAGCGCTAGGCATGGACAGGCGCACGGATG
CCTACAGCAGGTCGTCCTCAGTCTCCAGCCAGGAGGAGAAGTCGCTCCAC
GCGGAGGAGCTGGTTGGCAGGGGCATCCCCATCGAGCGAGTCGTCTCCTC
GGAGGGTGGCCGGCCCTCTGTGGACCTCTCCTTCCAGCCCTCGCAGCCCC
TGAGCAAGTCCAGCTCCTCTCCCGAGCTGCAGACTCTGCAGGACATCCTC
GGGGACCCTGGGGACAAGGCCGACGTGGGCCGGCTGAGCCCTGAGGTTAA
GGCCCGGTCACAGTCAGGGACCCTGGACGGGGAAAGTGCTGCCTGGTCGG
CCTCGGGCGAAGACAGTCGGGGCCAGCCCGAGGGTCCCTTGCCTTCCAGC
TCCCCCCGCTCGCCCAGTGGCCTCCGGCCCCGAGGTTACACCATCTCCGA
CTCGGCCCCATCACGCAGGGGCAAGAGAGTAGAGAGGGACGCCTTAAAGA
GCAGAGCCACAGCCTCCAATGCAGAGAAAGTGCCAGGCATCAACCCCAGT
TTCGTGTTCCTGCAGCTCTACCATTCCCCCTTCTTTGGCGACGAGTCAAA
CAAGCCAATCCTGCTGCCCAATGAGTCACAGTCCTTTGAGCGGTCGGTGC
AGCTCCTCGACCAGATCCCATCATACGACACCCACAAGATCGCCGTCCTG
TATGTTGGAGAAGGCCAGAGCAACAGCGAGCTCGCCATCCTGTCCAATGA
GCATGGCTCCTACAGGTACACGGAGTTCCTGACGGGCCTGGGCCGGCTCA
TCGAGCTGAAGGACTGCCAGCCGGACAAGGTGTACCTGGGAGGCCTGGAC
GTGTGTGGTGAGGACGGCCAGTTCACCTACTGCTGGCACGATGACATCAT
GCAAGCCGTCTTCCACATCGCCACCCTGATGCCCACCAAGGACGTGGACA
AGCACCGCTGCGACAAGAAGCGCCACCTGGGCAACGACTTTGTGTCCATT
GTCTACAATGACTCCGGTGAGGACTTCAAGCTTGGCACCATCAAGGGCCA
GTTCAACTTTGTCCACGTGATCGTCACCCCGCTGGACTACGAGTGCAACC
TGGTGTCCCTGCAGTGCAGGAAAGACATGGAGGGCCTTGTGGACACCAGC
GTGGCCAAGATCGTGTCTGACCGCAACCTGCCCTTCGTGGCCCGCCAGAT
GGCCCTGCACGCAAATATGGCCTCACAGGTGCATCATAGCCGCTCCAACC
CCACCGATATCTACCCCTCCAAGTGGATTGCCCGGCTCCGCCACATCAAG
CGGCTCCGCCAGCGGATCTGCGAGGAAGCCGCCTACTCCAACCCCAGCCT
ACCTCTGGTGCACCCTCCGTCCCATAGCAAAGCCCCTGCACAGACTCCAG
CCGAGCCCACACCTGGCTATGAGGTGGGCCAGCGGAAGCGCCTCATCTCC
TCGGTGGAGGACTTCACCGAGTTTGTG

Amino Acid Sequence

MAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSM
ECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHA
VLALLKAIVQGQGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNG
RHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQ
MICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVK
ELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVG
MALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYR
KELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEF
HGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALME
RFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDK
DHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDV
AAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKH
SYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYME
PERGSEKKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQCLKQE
SDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERL
RGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRC
ASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTL
ARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRC
RLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIA
RPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASL
GSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEF
LLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGV
HVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFL
GSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVR
RPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALY
KSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVM
EEGSPGEVPVLVEPPGLEDVEAALGMDRRTDAYSRSSSVSSQEEKSLHAE
ELVGRGIPIERVVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGD
PGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSP
RSPSGLRPRGYTISDSAPSRRGKRVERDALKSRATASNAEKVPGINPSFV
FLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYV
GEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVC
GEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVY
NDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVA
KIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRL
RQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSV
EDFTEFVVEKLAAADYKDDDDK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28896
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 41573. Isoform 1 produced by Q5 insertion of Val1272-Ala1294
• Low Temp: No