cDNA Clone IRAK2

• Plasmid: pFastbacDual 6HIS TEV GST 3C AVI IRAK2 192-507
• Parent Plasmid: pFastbac
• Expressed: 6HIS TEV GST 3C AVI IRAK2 192-507
• Tag: GST
• Tag: 6His
• Tag: AVI
• Species: Human

Sequence of Insert

GGCCTGAACGATATTTTTGAAGCGCAGAAAATTGAATGGCATGAAGGCAGCGGCGGCAGCGGCCTGGGATCCGCTGGAGACAGCCTTTTCTGGAGTGAGG
CAGACGTGGTCCAGGCAACCGATGACTTCAATCAAAACCGCAAAATCAGCCAGGGGACCTTTGCTGACGTCTACAGAGGGCACAGGCACGGGAAGCCATT
CGTCTTCAAGAAGCTCAGAGAGACAGCCTGTTCAAGTCCAGGATCAATCGAAAGATTCTTCCAGGCAGAGTTGCAGATTTGTCTTAGATGCTGCCACCCC
AATGTCTTACCTGTGCTGGGCTTCTGTGCTGCAAGACAGTTTCACAGCTTCATCTACCCCTACATGGCAAATGGTTCCCTACAGGACAGACTGCAGGGTC
AGGGTGGCTCGGACCCCCTCCCCTGGCCCCAGCGTGTCAGCATCTGCTCAGGGCTGCTCTGTGCCGTCGAGTACCTGCATGGTCTGGAGATCATCCACAG
CAACGTCAAGAGCTCTAATGTCTTGCTGGACCAAAATCTCACCCCCAAACTTGCTCACCCAATGGCTCATCTGTGTCCTGTCAACAAAAGGTCAAAATAC
ACCATGATGAAGACTCACCTGCTCCGGACGTCAGCCGCGTATCTGCCAGAGGATTTCATCCGGGTGGGGCAGCTGACAAAGCGAGTGGACATCTTCAGCT
GTGGAATAGTGTTGGCCGAGGTCCTCACGGGCATCCCTGCAATGGATAACAACCGAAGCCCGGTTTACCTGAAGGACTTACTCCTCAGTGATATTCCAAG
CAGCACCGCCTCGCTCTGCTCCAGGAAGACGGGCGTGGAGAACGTGATGGCAAAGGAGATCTGCCAGAAGTACCTGGAGAAGGGCGCAGGGAGGCTTCCG
GAGGACTGCGCCGAGGCCCTGGCCACGGCTGCCTGCCTGTGCCTGCGGAGGCGTAACACCAGCCTGCAGGAGGTGTGTGGCTCTGTGGCTGCTGTGGAAG
AGCGGCTCCGAGGTCGGGAGTAGTTGCTCCCTTGGAGTGGGCTTTCTGAGGGTACAGGCTCTTCTTCCAACACCCCAGAGGAAACAGACGACGTTGACAA
TTCCAGCCTTGATGCCTCCTCCTCCATGAGTGTGGCACCCTGGGCAGGGGCTGCCACCCCACTTCTCCCCACAGAGAATGGGGAAGGAAGGCTGCGGGTC
ATCGTGGGAAGGGAGGCTGACTCCTCCTCTGAGGCCTGTGTTGGCCTGGAGCCTCCCCAGGATGTTACAGAAACTTCGTGGCAAATTGAGATCAATGAGG
CCAAAAGGAAACTGATGGAGAATATTCTGCTCTACAAAGAGGAAAAAGTGGACAGCATTGAGCTCTTTGGCCCCTGAgcggccgc

Amino Acid Sequence

MHHHHHHENLYFQGMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCP
KERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAI
PQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPGLNDIFEAQKIEWHEGSGGSGLGSAGDSLFWSEADVVQATDDFNQNRKISQGTFADVY
RGHRHGKPFVFKKLRETACSSPGSIERFFQAELQICLRCCHPNVLPVLGFCAARQFHSFIYPYMANGSLQDRLQGQGGSDPLPWPQRVSICSGLLCAVEY
LHGLEIIHSNVKSSNVLLDQNLTPKLAHPMAHLCPVNKRSKYTMMKTHLLRTSAAYLPEDFIRVGQLTKRVDIFSCGIVLAEVLTGIPAMDNNRSPVYLK
DLLLSDIPSSTASLCSRKTGVENVMAKEICQKYLEKGAGRLPEDCAEALATAACLCLRRRNTSLQEVCGSVAAVEERLRGRE*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU56367
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please insert Avitag sequence (GLNDIFEAQKIEWHE) between 3C cleavage site and protein with 6 aa spacer (e.g. GSGGSG). DU51963 might be suitable template.
• Low Temp: No