cDNA Clone IRAK2

• Plasmid: pFastbac 6HIS TEV GST 3C AVI IRAK2 192-625
• Parent Plasmid: pFastbac
• Expressed: 6HIS TEV GST 3C AVI IRAK2 192-625
• Tag: GST
• Tag: 6His
• Tag: AVI
• Species: Human

Sequence of Insert

GGCCCGGCCTGAACGATATTTTTGAAGCGCAGAAAAGCGGCGGCAGCGGCCTGGGATCCGCTGGAGACAGCCTTTTCTGGAGTGAGGCAGACGTGGTCCA
GGCAACCGATGACTTCAATCAAAACCGCAAAATCAGCCAGGGGACCTTTGCTGACGTCTACAGAGGGCACAGGCACGGGAAGCCATTCGTCTTCAAGAAG
CTCAGAGAGACAGCCTGTTCAAGTCCAGGATCAATCGAAAGATTCTTCCAGGCAGAGTTGCAGATTTGTCTTAGATGCTGCCACCCCAATGTCTTACCTG
TGCTGGGCTTCTGTGCTGCAAGACAGTTTCACAGCTTCATCTACCCCTACATGGCAAATGGTTCCCTACAGGACAGACTGCAGGGTCAGGGTGGCTCGGA
CCCCCTCCCCTGGCCCCAGCGTGTCAGCATCTGCTCAGGGCTGCTCTGTGCCGTCGAGTACCTGCATGGTCTGGAGATCATCCACAGCAACGTCAAGAGC
TCTAATGTCTTGCTGGACCAAAATCTCACCCCCAAACTTGCTCACCCAATGGCTCATCTGTGTCCTGTCAACAAAAGGTCAAAATACACCATGATGAAGA
CTCACCTGCTCCGGACGTCAGCCGCGTATCTGCCAGAGGATTTCATCCGGGTGGGGCAGCTGACAAAGCGAGTGGACATCTTCAGCTGTGGAATAGTGTT
GGCCGAGGTCCTCACGGGCATCCCTGCAATGGATAACAACCGAAGCCCGGTTTACCTGAAGGACTTACTCCTCAGTGATATTCCAAGCAGCACCGCCTCG
CTCTGCTCCAGGAAGACGGGCGTGGAGAACGTGATGGCAAAGGAGATCTGCCAGAAGTACCTGGAGAAGGGCGCAGGGAGGCTTCCGGAGGACTGCGCCG
AGGCCCTGGCCACGGCTGCCTGCCTGTGCCTGCGGAGGCGTAACACCAGCCTGCAGGAGGTGTGTGGCTCTGTGGCTGCTGTGGAAGAGCGGCTCCGAGG
TCGGGAGACGTTGCTCCCTTGGAGTGGGCTTTCTGAGGGTACAGGCTCTTCTTCCAACACCCCAGAGGAAACAGACGACGTTGACAATTCCAGCCTTGAT
GCCTCCTCCTCCATGAGTGTGGCACCCTGGGCAGGGGCTGCCACCCCACTTCTCCCCACAGAGAATGGGGAAGGAAGGCTGCGGGTCATCGTGGGAAGGG
AGGCTGACTCCTCCTCTGAGGCCTGTGTTGGCCTGGAGCCTCCCCAGGATGTTACAGAAACTTCGTGGCAAATTGAGATCAATGAGGCCAAAAGGAAACT
GATGGAGAATATTCTGCTCTACAAAGAGGAAAAAGTGGACAGCATTGAGCTCTTTGGCCCCTAAgcggccgc

Amino Acid Sequence

MHHHHHHENLYFQGMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCP
KERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAI
PQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPGLNDIFEAQKSGGSGLGSAGDSLFWSEADVVQATDDFNQNRKISQGTFADVYRGHRHG
KPFVFKKLRETACSSPGSIERFFQAELQICLRCCHPNVLPVLGFCAARQFHSFIYPYMANGSLQDRLQGQGGSDPLPWPQRVSICSGLLCAVEYLHGLEI
IHSNVKSSNVLLDQNLTPKLAHPMAHLCPVNKRSKYTMMKTHLLRTSAAYLPEDFIRVGQLTKRVDIFSCGIVLAEVLTGIPAMDNNRSPVYLKDLLLSD
IPSSTASLCSRKTGVENVMAKEICQKYLEKGAGRLPEDCAEALATAACLCLRRRNTSLQEVCGSVAAVEERLRGRETLLPWSGLSEGTGSSSNTPEETDD
VDNSSLDASSSMSVAPWAGAATPLLPTENGEGRLRVIVGREADSSSEACVGLEPPQDVTETSWQIEINEAKRKLMENILLYKEEKVDSIELFGP*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU56369
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please insert Avitag sequence (GLNDIFEAQKIEWHE) between 3C cleavage site and protein with 6 aa spacer (e.g. GSGGSG). DU56039 might be suitable template.
• Low Temp: No