cDNA Clone IRAK3

• Plasmid: pFastbac 6HIS TEV GST 3C AVI IRAK3 145-596
• Parent Plasmid: pFastbac
• Expressed: 6HIS TEV GST 3C AVI IRAK3 145-596
• Tag: GST
• Tag: 6His
• Tag: AVI
• Species: Human

Sequence of Insert

GGCCCGGCCTGAACGATATTTTTGAAGCGCAGAAAATTGAATGGCATGAAGGCAGCGGCGGCAGCGGCAAAGGAGTACTGCTTAAATCTTCCATCAGCTT
TCAAAATATCATAGAAGGAACTAGAAATTTCCACAAAGACTTCCTAATTGGAGAAGGAGAGATTTTTGAGGTATACAGAGTGGAGATTCAAAACCTAACA
TATGCTGTCAAATTATTTAAACAGGAGAAAAAAATGCAGTGTAAGAAGCATTGGAAGAGGTTTTTATCTGAGCTTGAAGTTTTACTACTGTTTCATCACC
CAAACATACTAGAGTTGGCTGCATATTTTACAGAGACTGAGAAGTTCTGTCTGATTTATCCATACATGAGAAATGGAACACTTTTTGACAGATTGCAGTG
TGTAGGTGACACGGCCCCACTCCCTTGGCACATTCGAATCGGTATATTAATAGGAATATCCAAAGCCATTCACTACCTGCACAACGTTCAACCATGCTCG
GTCATCTGTGGCAGTATATCAAGTGCAAACATCCTTTTGGATGATCAGTTTCAACCCAAACTAACTGATTTTGCCATGGCACACTTCCGGTCCCACCTAG
AACATCAGAGTTGTACCATAAATATGACCAGCAGCAGCAGTAAACATCTGTGGTACATGCCAGAAGAGTACATCAGACAGGGGAAACTTTCCATTAAAAC
AGATGTCTACAGCTTTGGAATTGTAATAATGGAAGTTCTAACAGGATGTAGAGTAGTGTTAGATGATCCAAAACATATCCAGCTGCGGGATCTCCTTAGA
GAATTGATGGAGAAGAGAGGCCTGGATTCATGTCTCTCATTTCTAGATAAGAAAGTGCCTCCCTGCCCTCGGAATTTCTCTGCCAAGCTCTTCTGTTTGG
CAGGCCGGTGTGCTGCAACGCGGGCAAAGTTAAGACCATCAATGGATGAAGTTTTAAATACTCTTGAAAGTACTCAAGCCAGCTTGTATTTTGCTGAAGA
TCCTCCCACATCACTAAAGTCCTTCAGGTGTCCTTCTCCTCTATTCCTGGAGAATGTACCAAGTATTCCAGTGGAAGATGATGAAAGCCAGAATAACAAT
TTACTACCTTCTGATGAAGGCCTGAGGATAGACAGAATGACTCAGAAAACTCCTTTTGAATGCAGCCAGTCTGAGGTTATGTTTCTGAGCTTGGACAAAA
AGCCAGAGAGCAAGAGAAATGAGGAAGCTTGCAACATGCCCAGTTCTTCTTGTGAAGAAAGTTGGTTCCCAAAGTATATAGTTCCATCCCAGGACTTAAG
GCCCTATAAGGTAAATATAGATCCTTCTTCAGAAGCTCCAGGGCATTCTTGCAGGAGCAGGCCAGTGGAGAGCAGCTGTTCCTCCAAATTTTCCTGGGAT
GAATATGAACAGTACAAAAAAGAATAAGCGGCCGC

Amino Acid Sequence

MHHHHHHENLYFQGMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCP
KERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAI
PQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPGLNDIFEAQKIEWHEGSGGSGKGVLLKSSISFQNIIEGTRNFHKDFLIGEGEIFEVYR
VEIQNLTYAVKLFKQEKKMQCKKHWKRFLSELEVLLLFHHPNILELAAYFTETEKFCLIYPYMRNGTLFDRLQCVGDTAPLPWHIRIGILIGISKAIHYL
HNVQPCSVICGSISSANILLDDQFQPKLTDFAMAHFRSHLEHQSCTINMTSSSSKHLWYMPEEYIRQGKLSIKTDVYSFGIVIMEVLTGCRVVLDDPKHI
QLRDLLRELMEKRGLDSCLSFLDKKVPPCPRNFSAKLFCLAGRCAATRAKLRPSMDEVLNTLESTQASLYFAEDPPTSLKSFRCPSPLFLENVPSIPVED
DESQNNNLLPSDEGLRIDRMTQKTPFECSQSEVMFLSLDKKPESKRNEEACNMPSSSCEESWFPKYIVPSQDLRPYKVNIDPSSEAPGHSCRSRPVESSC
SSKFSWDEYEQYKKE*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU56368
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please insert Avitag sequence (GLNDIFEAQKIEWHE) between 3C cleavage site and protein with 6 aa spacer (e.g. GSGGSG). DU51996 might be suitable template.
• Low Temp: No