cDNA Clone Fam83D

• Plasmid: pcDNAfrtTO-GFP Fam83D T370E T543E
• Parent Plasmid: pcDNAfrtTO-GFP
• Expressed: GFP-Fam83D T370E T543E
• Genbank: NM_030919.2
• Tag: GFP
• Species: Human

Sequence of Insert

ggatccatggcgcgggcctgtttgattcaaaggttgcctataaagcggga
ctgcacgccggtttttgtccgagggctgtcgagtccgagcgccgccatgg
ctctgctgtccgagggcctggacgaggtgcccgccgcctgcctgtcgccg
tgcgggccgcccaacccgaccgagctgttcagcgagtcacggcgcctggc
tctggaggagctggtggcgggcggccccgaagccttcgcggccttcctgc
gacgcgagcgcctggctcgtttcctgaaccccgatgaggtgcacgccatt
ctgcgcgcggcggagaggccgggagaggagggcgcggcggcggcggcggc
ggccgaggactcgttcggctcctcgcacgactgctcttcgggcacctact
tccccgagcagtcggacctggagccaccgctgttggagcttggctggccc
gccttctaccagggcgcctaccgcggcgccacgcgtgtcgagacgcactt
ccagccccgcggcgctggcgaaggtggcccctacggctgcaaggacgctc
tgcgccagcagctccgctcggcgcgagaggtgattgcagtggtcatggac
gtgttcacagacatcgacatcttcagagacctgcaagaaatatgcaggaa
acagggagttgctgtgtatatccttctggaccaggctctcctctctcaat
ttctggatatgtgcatggatctgaaagttcatcctgaacaggaaaagtta
atgacagttcggactatcacaggaaatatctactatgcaaggtcaggaac
taagattattgggaaggttcacgaaaagttcacgttgattgatggcatcc
gcgtggcaacaggctcctacagttttacatggacggatggcaaattaaac
agcagtaacttggtaattctgtctggccaagtggttgaacactttgatct
ggagttccgaatcctgtatgcccagtccaagcccatcagccccaaactcc
tgtctcacttccagagcagcaacaagtttgatcacctcaccaaccgaaaa
ccacagtccaaggagctcaccctgggcaacctgctgcggatgcggctggc
taggctgtcaagtgagcccaggaaggcggacctggacccagagatgcccg
cagagggcaaggcagagcgcaagccccatgactgtgagtcctctactgtt
agtgaggaagactacttcagcagccacagggacgagctccagagcagaaa
ggccattgacgctgccactcaaacagagccaggagaggagatgccagggc
tgagtgtgagtgaggtgggaacacaaaccagcatcaccacagcatgtgct
ggtacccagactgcagtcatcaccaggatagcaagctctcaaaccacgat
ttggtccagatcgaccactactcagactgacatggatgagaacattctct
ttcctcgaggaactcaatctacagaagggtcaccagtctcaaaaatgtct
gtatcgagatcttccagtttgaagtcttcctcctctgtgtcttcccaagg
ctctgtggcaagctccactggttctcccgcttccatcagaaccactgact
tccacaatcctggctatcccaagtacctgggcGAGccccacctggaactg
tacttgagtgactcacttagaaacttgaacaaagagcggcaattccactt
cgctggtatcaggtcccggctcaaccacatgctggctatgctgtcaagga
gaacactctttactgaaaaccaccttggccttcattctggcaatttcagc
agagttaatttgcttgctgttagagatgtagcactttatccttcctatca
gtaaGTCGACTCGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMARACL
IQRLPIKRDCTPVFVRGLSSPSAAMALLSEGLDEVPAACLSPCGPPNPTE
LFSESRRLALEELVAGGPEAFAAFLRRERLARFLNPDEVHAILRAAERPG
EEGAAAAAAAEDSFGSSHDCSSGTYFPEQSDLEPPLLELGWPAFYQGAYR
GATRVETHFQPRGAGEGGPYGCKDALRQQLRSAREVIAVVMDVFTDIDIF
RDLQEICRKQGVAVYILLDQALLSQFLDMCMDLKVHPEQEKLMTVRTITG
NIYYARSGTKIIGKVHEKFTLIDGIRVATGSYSFTWTDGKLNSSNLVILS
GQVVEHFDLEFRILYAQSKPISPKLLSHFQSSNKFDHLTNRKPQSKELTL
GNLLRMRLARLSSEPRKADLDPEMPAEGKAERKPHDCESSTVSEEDYFSS
HRDELQSRKAIDAATQTEPGEEMPGLSVSEVGTQTSITTACAGTQTAVIT
RIASSQTTIWSRSTTTQTDMDENILFPRGTQSTEGSPVSKMSVSRSSSLK
SSSSVSSQGSVASSTGSPASIRTTDFHNPGYPKYLGEPHLELYLSDSLRN
LNKERQFHFAGIRSRLNHMLAMLSRRTLFTENHLGLHSGNFSRVNLLAVR
DVALYPSYQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU29164
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: can be excised Bam-Sal1 or Bam-Not1
• Low Temp: No