cDNA Clone ubiquitin

• Synonyms: X
• Plasmid: pET15-UBIQUITIN-6HIS TAG31
• Parent Plasmid: pET156P-1
• Expressed: ubiquitin-His TAG31
• Genbank: X
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCTAGGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCCGAGGTGGGCATCACCATCACCATCACTAAGCGGCCGC

Amino Acid Sequence

MGSMQIFVKTLTGKTITLEVEPSDTIENVKAKI*DKEGIPPDQQRLIFAGKQL
EDGRTLSDYNIQKESTLHLVLRLRGGHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pET15-UBIQUITIN-6HIS TAG31145.61 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU29178
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N-ter 6His deleted. C-terminal Hisx6 tag immediately follows GG (retain TAA stop codon). Clone has 3 extra residues (MGS) upstream of ubiquitin Met1
• Low Temp: No