cDNA Clone MINDY4

• Synonyms: FAM188B
• Plasmid: pcDNA FRT TO MINDY4 BirA HA
• Parent Plasmid: pcDNA FRT TO c-BirA HA
• Expressed: MINDY4_BirA_HA
• Genbank: Q4G0A6-1

Sequence of Insert

AGATCTATGGACAGCCTCTTCGTGGAGGAGGTGGCCGCCTCCTTGGTCAGGGAGTTCCTCAGCAGAAAGGGCTTAAAGAAGACATGTGTGACCATGGACCAGGAACGCCCACGCTCTGACCTCAGCATAAACAACAGAAATGATCTTCGAAAGGTTTTGCATCTTGAATTTCTCTATAAGGAGAACAAGGCAAAGGAAAATCCTCTAAAAACAAGCCTTGAACTCATCACCAGATACTTTCTGGATCACTTTGGAAATACGGCTAACAATTTCACTCAAGATACCCCAATCCCTGCACTCTCAGTTCCAAAGAAAAATAACAAAGTGCCATCAAGATGCTCAGAGACTACACTGGTAAATATATATGACCTTTCAGATGAAGATGCAGGATGGAGAACATCATTGTCAGAAACAAGCAAAGCCAGACATGACAATCTTGATGGAGATGTACTTGGTAATTTTGTATCATCTAAAAGGCCCCCGCACAAAAGTAAGCCCATGCAGACGGTCCCGGGTGAAACTCCTGTGTTGACTTCTGCATGGGAGAAGATAGACAAGCTTCACTCGGAGCCTTCCTTGGATGTGAAGAGGATGGGAGAGAATTCCAGGCCAAAGTCTGGTCTGATTGTGCGAGGCATGATGTCTGGGCCCATCGCCAGCTCCCCACAGGATTCTTTTCACAGACACTATCTGAGACGGTCCTCACCGTCAAGCAGCTCCACCCAACCCCAAGAAGAGAGCCGGAAGGTCCCTGAGCTCTTTGTCTGCACCCAACAGGACATTCTGGCTTCGAGCAACAGCTCCCCCTCCAGGACCTCCCTGGGTCAGCTTAGTGAACTGACCGTAGAAAGGCAGAAAACCACTGCCAGCAGCCCTCCCCATCTGCCCAGCAAACGGCTGCCCCCATGGGACAGGGCCAGGCCGAGGGATCCCTCCGAGGACACCCCAGCAGTGGACGGCAGCACAGACACGGACAGGATGCCCTTGAAGCTCTACTTGCCTGGTGGTAATTCCAGGATGACCCAGGAGAGGCTGGAAAGAGCGTTCAAACGGCAGGGCAGCCAGCCCGCACCTGTCAGGAAAAATCAGTTGCTGCCGTCTGACAAGGTGGATGGTGAGCTGGGTGCCCTGCGGCTCGAGGATGTGGAGGATGAGTTGATAAGGGAAGAGGTCATCCTGTCGCCAGTCCCATCAGTGCTCAAGTTGCAGACAGCATCAAAACCAATTGACCTCTCAGTAGCAAAGGAAATAAAGACCCTTCTGTTTGGTTCCAGCTTTTGCTGTTTCAATGAAGAATGGAAACTTCAGAGTTTTTCCTTTAGTAACACAGCCTCATTAAAATACGGCATAGTGCAGAACAAGGGTGGTCCTTGCGGAGTCCTGGCAGCTGTCCAAGGCTGTGTCCTACAGAAACTCCTGTTTGAAGGAGATAGCAAAGCCGACTGTGCTCAGGGACTGCAGCCTTCAGATGCCCACCGGACCCGCTGCCTCGTCCTGGCCCTCGCAGACATTGTGTGGCGGGCAGGGGGCCGAGAGAGAGCCGTTGTTGCACTGGCTTCGAGAACACAGCAGTTCAGTCCAACAGGGAAATACAAAGCAGATGGAGTCTTAGAAACACTTACGCTTCACAGTTTGACCTGCTATGAGGACCTGGTGACTTTTCTTCAACAAAGCATTCATCAGTTTGAAGTGGGCCCCTATGGCTGCATCCTGCTCACCCTTTCTGCCATCCTGTCCAGGTCTACAGAGCTCATCCGCCAGGACTTTGATGTCCCCACCAGCCACCTGATTGGAGCACATGGCTACTGTACACAGGAACTTGTCAATCTGCTCCTGACTGGGAAAGCTGTGTCCAACGTTTTCAACGATGTGGTTGAGCTGGATTCTGGGGATGGGAACATCACACTTCTCAGAGGCATTGCTGCACGCAGTGATATTGGCTTCTTATCTCTCTTTGAGCATTACAACATGTGCCAGGTTGGCTGCTTCCTGAAGACCCCGAGGTTCCCCATCTGGGTGGTTTGCAGTGAGAGCCACTTCAGCATCCTCTTTAGCCTGCAGCCGGGGCTCCTGCGTGACTGGAGGACTGAGAGGCTCTTTGACTTGTACTACTACGATGGCCTGGCCAACCAGCAGGAGCAGATCCGGCTGACCATTGACACCACCCAAACCATCTCTGAGGACACAGACAACGACCTTGTCCCACCCCTCGAGCTCTGCATCAGAACCAAGTGGAAGGGGGCATCAGTGAACTGGAACGGCTCAGACCCCATCCTGGCGGCCGC

Amino Acid Sequence

MDSLFVEEVAASLVREFLSRKGLKKTCVTMDQERPRSDLSINNRNDLRKVLHLEFLYKENKAKENPLKTSLELITRYFLDHFGNTANNFTQDTPIPALSVPKKNNKVPSRCSETTLVNIYDLSDEDAGWRTSLSETSKARHDNLDGDVLGNFVSSKRPPHKSKPMQTVPGETPVLTSAWEKIDKLHSEPSLDVKRMGENSRPKSGLIVRGMMSGPIASSPQDSFHRHYLRRSSPSSSSTQPQEESRKVPELFVCTQQDILASSNSSPSRTSLGQLSELTVERQKTTASSPPHLPSKRLPPWDRARPRDPSEDTPAVDGSTDTDRMPLKLYLPGGNSRMTQERLERAFKRQGSQPAPVRKNQLLPSDKVDGELGALRLEDVEDELIREEVILSPVPSVLKLQTASKPIDLSVAKEIKTLLFGSSFCCFNEEWKLQSFSFSNTASLKYGIVQNKGGPCGVLAAVQGCVLQKLLFEGDSKADCAQGLQPSDAHRTRCLVLALADIVWRAGGRERAVVALASRTQQFSPTGKYKADGVLETLTLHSLTCYEDLVTFLQQSIHQFEVGPYGCILLTLSAILSRSTELIRQDFDVPTSHLIGAHGYCTQELVNLLLTGKAVSNVFNDVVELDSGDGNITLLRGIAARSDIGFLSLFEHYNMCQVGCFLKTPRFPIWVVCSESHFSILFSLQPGLLRDWRTERLFDLYYYDGLANQQEQIRLTIDTTQTISEDTDNDLVPPLELCIRTKWKGASVNWNGSDPILAAAMKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEKYPYDVPDYA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU59100
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Kindly clone MINDY4 with BirA R118G_HA fusion in pCDNA5 FRT/TO (MINDY4_BirA R118G_HA). Kindly keep the HA tag at the C-terminus along with BirA and let the N-terminus of the protein be free.
• Low Temp: No