cDNA Clone TRIM25

• Plasmid: pGEX6P-1-TRIM25
• Parent Plasmid: pGEX6P-1-
• Expressed: GST-TRIM25
• Genbank: NP_005073.2
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGGCAGAGCTGTGCCCCCTGGCCGAGGAGCTGTCGTGCTC CATCTGCCTGGAGCCCTTCAAGGAGCCGGTCACCACTCCGTGCGGCCACA ACTTCTGCGGGTCGTGCCTGAATGAGACGTGGGCAGTCCAGGGCTCGCCA TACCTGTGCCCGCAGTGCCGCGCCGTCTACCAGGCGCGACCGCAGCTGCA CAAGAACACGGTGCTGTGCAACGTGGTGGAGCAGTTCCTGCAGGCCGACC TGGCCCGGGAGCCACCCGCCGACGTCTGGACGCCGCCCGCCCGCGCCTCT GCACCCAGCCCGAATGCCCAGGTGGCCTGCGACCACTGCCTGAAGGAGGC CGCCGTGAAGACGTGCTTGGTGTGCATGGCCTCCTTCTGTCAGGAGCACC TGCAGCCGCACTTCGACAGCCCCGCCTTCCAGGACCACCCGCTGCAGCCG CCCGTTCGCGACCTGTTGCGCCGCAAATGTTCCCAGCACAATCGGCTGCG GGAATTTTTCTGCCCCGAGCACAGCGAGTGCATCTGCCACATCTGCCTGG TGGAGCATAAGACCTGCTCTCCCGCGTCCCTGAGCCAGGCCAGCGCCGAC CTGGAGGCCACCCTGAGGCACAAACTAACTGTCATGTACAGTCAGATCAA CGGGGCGTCGAGAGCACTGGATGATGTGAGAAACAGGCAGCAGGATGTGC GGATGACTGCAAACAGAAAGGTGGAGCAGCTACAACAAGAATACACGGAA ATGAAGGCTCTCTTGGACGCCTCAGAGACCACCTCGACAAGGAAGATAAA GGAAGAGGAGAAGAGGGTCAACAGCAAGTTTGACACCATTTATCAGATTC TCCTCAAGAAGAAGAGTGAGATCCAGACCTTGAAGGAGGAGATTGAACAG AGCCTGACCAAGAGGGATGAGTTCGAGTTTCTGGAGAAAGCATCAAAACT GCGAGGAATCTCAACAAAGCCAGTCTACATCCCCGAGGTGGAACTGAACC ACAAGCTGATAAAAGGCATCCACCAGAGCACCATAGACCTCAAAAACGAG CTGAAGCAGTGCATCGGGCGGCTCCAGGAGCTCACCCCCAGTTCAGGTGA CCCTGGAGAGCATGACCCAGCGTCCACACACAAATCCACACGCCCTGTGA AGAAGGTCTCCAAAGAGGAAAAGAAATCCAAGAAACCTCCCCCTGTCCCT GCCTTACCCAGCAAGCTTCCCACGTTTGGAGCCCCGGAACAGTTAGTGGA TTTAAAACAAGCTGGCTTGGAGGCTGCAGCCAAAGCCACCAGCTCACATC CGAACTCAACATCTCTCAAGGCCAAGGTGCTGGAGACCTTCCTGGCCAAG TCCAGACCTGAGCTCCTGGAGTATTACATTAAAGTCATCCTGGACTACAA CACCGCCCACAACAAAGTGGCTCTGTCAGAGTGCTATACAGTAGCTTCTG TGGCTGAGATGCCTCAGAACTACCGGCCGCATCCCCAGAGGTTCACATAC TGCTCTCAGGTGCTGGGCCTGCACTGCTACAAGAAGGGGATCCACTACTG GGAGGTGGAGCTGCAGAAGAACAACTTCTGTGGGGTAGGCATCTGCTACG GAAGCATGAACCGGCAGGGCCCAGAAAGCAGGCTCGGCCGCAACAGCGCC TCCTGGTGCGTGGAGTGGTTCAACACCAAGATCTCTGCCTGGCACAATAA CGTGGAGAAAACCCTGCCCTCCACCAAGGCCACGCGGGTGGGCGTGCTTC TCAACTGTGACCACGGCTTTGTCATCTTCTTCGCTGTTGCCGACAAGGTC CACCTGATGTATAAGTTCAGGGTGGACTTTACTGAGGCTTTGTACCCGGC TTTCTGGGTATTTTCTGCTGGTGCCACACTCTCCATCTGCTCCCCCAAGT AGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMAELCPLA
EELSCSICLEPFKEPVTTPCGHNFCGSCLNETWAVQGSPYLCPQCRAVYQ
ARPQLHKNTVLCNVVEQFLQADLAREPPADVWTPPARASAPSPNAQVACD
HCLKEAAVKTCLVCMASFCQEHLQPHFDSPAFQDHPLQPPVRDLLRRKCS
QHNRLREFFCPEHSECICHICLVEHKTCSPASLSQASADLEATLRHKLTV
MYSQINGASRALDDVRNRQQDVRMTANRKVEQLQQEYTEMKALLDASETT
STRKIKEEEKRVNSKFDTIYQILLKKKSEIQTLKEEIEQSLTKRDEFEFL
EKASKLRGISTKPVYIPEVELNHKLIKGIHQSTIDLKNELKQCIGRLQEL
TPSSGDPGEHDPASTHKSTRPVKKVSKEEKKSKKPPPVPALPSKLPTFGA
PEQLVDLKQAGLEAAAKATSSHPNSTSLKAKVLETFLAKSRPELLEYYIK
VILDYNTAHNKVALSECYTVASVAEMPQNYRPHPQRFTYCSQVLGLHCYK
KGIHYWEVELQKNNFCGVGICYGSMNRQGPESRLGRNSASWCVEWFNTKI
SAWHNNVEKTLPSTKATRVGVLLNCDHGFVIFFAVADKVHLMYKFRVDFT
EALYPAFWVFSAGATLSICSPK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU29274
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: SNP at L358 (source derived)
• Low Temp: No