cDNA Clone Lysozyme - IRAK3

• Plasmid: pFastbac 6HIS TEV GST-Chicken Lysozyme-(K19-R143)-IRAK3 145-454
• Parent Plasmid: pFastbac 6HIS TEV GST
• Genbank: AH002457.2 (chicken lysozyme), NM_007199.2 → NP_009130.2 (IRAK3)
• Tag: GST

Sequence of Insert

GGGCCCCTGaaagtctttggacgatgtgagctggcagcggctatgaagcgtcacggacttgataactatcggggatacagcctgggaaactgggtgtgtgttgcaaaattcgagagtaacttcaacacccaggctacaaaccgtaacaccgatgggagtaccgactacggaatcctacagatcaacagccgctggtggtgcaacgatggcaggaccccaggctccaggaacctgtgcaacatcccgtgctcagccctgctgagctcagacataacagcgagcgtgaactgcgcgaagaagatcgtcagcgatggaaacggcatgagcgcgtgggtcgcctggcgcaaccgctgcaagggtaccgacgtccaggcgtggatcagaGGATCC

Amino Acid Sequence

MHHHHHHENLYFQGMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPKVFGRCELAAAMKRHGLDNYRG
YSLGNWVCVAKFESNFNTQATNRNTDGSTDYGILQINSRWWCNDGRTPGS
RNLCNIPCSALLSSDITASVNCAKKIVSDGNGMSAWVAWRNRCKGTDVQA
WIRGSKGVLLKSSISFQNIIEGTRNFHKDFLIGEGEIFEVYRVEIQNLTY
AVKLFKQEKKMQCKKHWKRFLSELEVLLLFHHPNILELAAYFTETEKFCL
IYPYMRNGTLFDRLQCVGDTAPLPWHIRIGILIGISKAIHYLHNVQPCSV
ICGSISSANILLDDQFQPKLTDFAMAHFRSHLEHQSCTINMTSSSSKHLW
YMPEEYIRQGKLSIKTDVYSFGIVIMEVLTGCRVVLDDPKHIQLRDLLRE
LMEKRGLDSCLSFLDKKVPPCPRNFSAKLFCLAGRCAATRAKLRPSMDEV
LNTLESTQASLYFAE*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU29240
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: GeneArt chicken lysozyme (K19-R143) fragment into Parental 56013; IRAK3 contains listed SNP V147. Clone truncated to E454 by internal stop (derived from source); *** still to add 6His-TEV to cassette*
• Low Temp: No