cDNA Clone CHFR

• Synonyms: E3 ubiquitin-protein ligase CHFR isoform 1, RNF116, RNF196, checkpoint with forkhead and ring finger domains
• Plasmid: pS-B-CHFR isoform 1 precursor
• Parent Plasmid: pS-B
• Genbank: NM_001161346.1 → NP_001154818.1
• Species: Human

Sequence of Insert

GGATCCATGGAGCGGCCCGAGGAAGGCAAGCAGTCGCCGCCGCCGCAGCC
CTGGGGACGGCTCCTGCGTCTGGGCGCGGAGGAGGGCGAGCCGCACGTCC
TCCTGAGGAAGCGGGAGTGGACCATCGGGCGGAGACGAGGTTGCGACCTT
TCCTTCCCCAGCAATAAACTGGTCTCTGGAGATCACTGTAGAATTGTAGT
GGATGAAAAATCAGGTCAGGTGACACTGGAAGATACCAGCACCAGTGGAA
CAGTGATTAACAAGCTGAAGGTTGTTAAGAAGCAGACATGCCCTTTACAG
ACTGGGGATGTCATCTACTTGGTGTACAGGAAGAATGAACCGGAACACAA
CGTGGCATACCTCTATGAATCTTTAAGTGAAAAGCAAGGCATGACACAAG
AATCCTTTGAAGCTAACAAGGAAAATGTGTTCCATGGGACCAAAGATACC
TCAGGTGCAGGTGCAGGGCGAGGGGCCGATCCCCGGGTCCCTCCGTCGTC
GCCCGCCACTCAGGTGTGCTTTGAGGAACCACAGCCATCAACATCGACGT
CAGACCTCTTCCCCACAGCCTCGGCCTCTTCCACGGAGCCTTCTCCTGCA
GGGCGAGAGCGTTCCTCCAGTTGTGGGTCTGGGGGTGGTGGCATCTCCCC
TAAAGGAAGTGGTCCCTCTGTGGCAAGTGATGAAGTCTCCAGCTTTGCCT
CAGCTCTCCCAGACAGAAAGACTGCGTCCTTTTCGTCGTTGGAACCCCAG
GATCAGGAGGATTTGGAGCCCGTGAAGAAGAAAATGAGAGGAGATGGGGA
CCTTGACCTGAACGGGCAGTTGTTGGTCGCACAACCGCGTAGAAATGCCC
AAACCGTCCACGAGGACGTCAGAGCAGCGGCTGGGAAGCCAGACAAGATG
GAGGAGACGCTGACATGCATCATCTGCCAGGACCTGCTGCACGACTGCGT
GAGTTTGCAGCCCTGCATGCACACGTTCTGCGCGGCTTGCTACTCGGGCT
GGATGGAGCGCTCGTCCCTGTGTCCTACCTGCCGCTGTCCCGTGGAGCGG
ATCTGTAAAAACCACATCCTCAACAACCTCGTGGAAGCATACCTCATCCA
GCATCCAGACAAGAGTCGCAGTGAAGAAGATGTGCAAAGTATGGATGCCA
GGAATAAAATCACTCAAGACATGCTGCAGCCCAAAGTCAGGCGGTCTTTT
TCTGATGAAGAAGGGAGTTCAGAGGACCTGCTGGAGCTGTCAGACGTTGA
CAGTGAGTCCTCAGACATTAGCCAGCCATACGTCGTGTGCCGGCAGTGTC
CTGAGTACAGAAGGCAGGCGGCGCAGCCTCCCCACTGCCCAGCACCCGAG
GGCGAGCCAGGAGCCCCACAGGCCCTGGGGGATGCACCCTCCACGTCCGT
CAGCCTGACGACAGTCCAGGATTACGTGTGCCCTCTGCAAGGAAGCCACG
CCCTGTGCACCTGCTGCTTCCAGCCCATGCCCGACCGGAGAGCGGAGCGC
GAGCAGGACCCGCGTGTCGCCCCTCAGCAGTGTGCGGTCTGCCTGCAGCC
TTTCTGCCACCTGTACTGGGGCTGCACCCGGACCGGCTGCTACGGCTGCC
TGGCCCCGTTTTGTGAGCTCAACCTGGGTGACAAGTGTCTGGACGGCGTG
CTGAACAACAACAGCTACGAGTCAGACATCCTGAAGAATTACCTGGCAAC
CAGAGGTTTGACATGGAAAAACATGTTGACCGAGAGCCTCGTGGCTCTCC
AGCGGGGAGTGTTTCTGCTGTCTGATTACAGAGTCACGGGAGACACCGTT
CTGTGTTACTGCTGTGGCCTGCGCAGCTTCCGTGAGCTGACCTATCAGTA
TCGGCAGAACATTCCTGCTTCCGAGTTGCCAGTGGCCGTAACATCCCGTC
CTGACTGCTACTGGGGCCGTAACTGCCGCACTCAGGTGAAAGCTCACCAC
GCCATGAAATTCAATCATATCTGTGAACAGACAAGGTTCAAAAACTAAGC
GGCCGC

Amino Acid Sequence

MERPEEGKQSPPPQPWGRLLRLGAEEGEPHVLLRKREWTIGRRRGCDLSF
PSNKLVSGDHCRIVVDEKSGQVTLEDTSTSGTVINKLKVVKKQTCPLQTG
DVIYLVYRKNEPEHNVAYLYESLSEKQGMTQESFEANKENVFHGTKDTSG
AGAGRGADPRVPPSSPATQVCFEEPQPSTSTSDLFPTASASSTEPSPAGR
ERSSSCGSGGGGISPKGSGPSVASDEVSSFASALPDRKTASFSSLEPQDQ
EDLEPVKKKMRGDGDLDLNGQLLVAQPRRNAQTVHEDVRAAAGKPDKMEE
TLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERIC
KNHILNNLVEAYLIQHPDKSRSEEDVQSMDARNKITQDMLQPKVRRSFSD
EEGSSEDLLELSDVDSESSDISQPYVVCRQCPEYRRQAAQPPHCPAPEGE
PGAPQALGDAPSTSVSLTTVQDYVCPLQGSHALCTCCFQPMPDRRAEREQ
DPRVAPQQCAVCLQPFCHLYWGCTRTGCYGCLAPFCELNLGDKCLDGVLN
NNSYESDILKNYLATRGLTWKNMLTESLVALQRGVFLLSDYRVTGDTVLC
YCCGLRSFRELTYQYRQNIPASELPVAVTSRPDCYWGRNCRTQVKAHHAM
KFNHICEQTRFKN*

• Vector Type: Bacterial
• Antibiotic: Amp; Kan
• DU Number: DU29265
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: insertion of E135-K146 to create isoform 1 precursor.; Silent change at R382 (AGA > AGG) and delta Ala470 (isoform 1 numbering) compared to isoform 1 NCBI ref
• Low Temp: No