cDNA Clone CHFR

• Synonyms: E3 ubiquitin-protein ligase CHFR isoform 1, RNF116, RNF196, checkpoint with forkhead and ring finger domains
• Plasmid: pGEX6P-1-CHFR isoform 3
• Parent Plasmid: pS-B
• Expressed: GST-CHFR isoform 3
• Genbank: NM_001161346.1 → NP_001154818.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGAGCGGCCCGAGGAAGGCAAGCAGTCGCCGCCGCCGCAGCC
CTGGGGACGGCTCCTGCGTCTGGGCGCGGAGGAGGGCGAGCCGCACGTCC
TCCTGAGGAAGCGGGAGTGGACCATCGGGCGGAGACGAGGTTGCGACCTT
TCCTTCCCCAGCAATAAACTGGTCTCTGGAGATCACTGTAGAATTGTAGT
GGATGAAAAATCAGGTCAGGTGACACTGGAAGATACCAGCACCAGTGGAA
CAGTGATTAACAAGCTGAAGGTTGTTAAGAAGCAGACATGCCCTTTACAG
ACTGGGGATGTCATCTACTTGGTGTACAGGAAGAATGAACCGGAACACAA
CGTGGCATACCTCTATGAATCTTTAAGTGAAAAGCAAGGCATGACACAAG
AATCCTTTGATACCTCAGGTGCAGGTGCAGGGCGAGGGGCCGATCCCCGG
GTCCCTCCGTCGTCGCCCGCCACTCAGGTGTGCTTTGAGGAACCACAGCC
ATCAACATCGACGTCAGACCTCTTCCCCACAGCCTCGGCCTCTTCCACGG
AGCCTTCTCCTGCAGGGCGAGAGCGTTCCTCCAGTTGTGGGTCTGGGGGT
GGTGGCATCTCCCCTAAAGGAAGTGGTCCCTCTGTGGCAAGTGATGAAGT
CTCCAGCTTTGCCTCAGCTCTCCCAGACAGAAAGACTGCGTCCTTTTCGT
CGTTGGAACCCCAGGATCAGGAGGATTTGGAGCCCGTGAAGAAGAAAATG
AGAGGAGATGGGGACCTTGACCTGAACGGGCAGTTGTTGGTCGCACAACC
GCGTAGAAATGCCCAAACCGTCCACGAGGACGTCAGAGCAGCGGCTGGGA
AGCCAGACAAGATGGAGGAGACGCTGACATGCATCATCTGCCAGGACCTG
CTGCACGACTGCGTGAGTTTGCAGCCCTGCATGCACACGTTCTGCGCGGC
TTGCTACTCGGGCTGGATGGAGCGCTCGTCCCTGTGTCCTACCTGCCGCT
GTCCCGTGGAGCGGATCTGTAAAAACCACATCCTCAACAACCTCGTGGAA
GCATACCTCATCCAGCATCCAGACAAGAGTCGCAGTGAAGAAGATGTGCA
AAGTATGGATGCCAGGAATAAAATCACTCAAGACATGCTGCAGCCCAAAG
TCAGGCGGTCTTTTTCTGATGAAGAAGGGAGTTCAGAGGACCTGCTGGAG
CTGTCAGACGTTGACAGTGAGTCCTCAGACATTAGCCAGCCATACGTCGT
GTGCCGGCAGTGTCCTGAGTACAGAAGGCAGGCGGCGCAGCCTCCCCACT
GCCCAGCACCCGAGGGCGAGCCAGGAGCCCCACAGGCCCTGGGGGATGCA
CCCTCCACGTCCGTCAGCCTGACGACAGTCCAGGATTACGTGTGCCCTCT
GCAAGGAAGCCACGCCCTGTGCACCTGCTGCTTCCAGCCCATGCCCGACC
GGAGAGCGGAGCGCGAGCAGGACCCGCGTGTCGCCCCTCAGCAGTGTGCG
GTCTGCCTGCAGCCTTTCTGCCACCTGTACTGGGGCTGCACCCGGACCGG
CTGCTACGGCTGCCTGGCCCCGTTTTGTGAGCTCAACCTGGGTGACAAGT
GTCTGGACGGCGTGCTGAACAACAACAGCTACGAGTCAGACATCCTGAAG
AATTACCTGGCAACCAGAGGTTTGACATGGAAAAACATGTTGACCGAGAG
CCTCGTGGCTCTCCAGCGGGGAGTGTTTCTGCTGTCTGATTACAGAGTCA
CGGGAGACACCGTTCTGTGTTACTGCTGTGGCCTGCGCAGCTTCCGTGAG
CTGACCTATCAGTATCGGCAGAACATTCCTGCTTCCGAGTTGCCAGTGGC
CGTAACATCCCGTCCTGACTGCTACTGGGGCCGTAACTGCCGCACTCAGG
TGAAAGCTCACCACGCCATGAAATTCAATCATATCTGTGAACAGACAAGG
TTCAAAAACTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMERPEEGKQSPPPQPWGRL
LRLGAEEGEPHVLLRKREWTIGRRRGCDLSFPSNKLVSGDHCRIVVDEKS
GQVTLEDTSTSGTVINKLKVVKKQTCPLQTGDVIYLVYRKNEPEHNVAYL
YESLSEKQGMTQESFDTSGAGAGRGADPRVPPSSPATQVCFEEPQPSTST
SDLFPTASASSTEPSPAGRERSSSCGSGGGGISPKGSGPSVASDEVSSFA
SALPDRKTASFSSLEPQDQEDLEPVKKKMRGDGDLDLNGQLLVAQPRRNA
QTVHEDVRAAAGKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSG
WMERSSLCPTCRCPVERICKNHILNNLVEAYLIQHPDKSRSEEDVQSMDA
RNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYVVCRQC
PEYRRQAAQPPHCPAPEGEPGAPQALGDAPSTSVSLTTVQDYVCPLQGSH
ALCTCCFQPMPDRRAEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCYGC
LAPFCELNLGDKCLDGVLNNNSYESDILKNYLATRGLTWKNMLTESLVAL
QRGVFLLSDYRVTGDTVLCYCCGLRSFRELTYQYRQNIPASELPVAVTSR
PDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU29270
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent change at R382 (AGA > AGG) and delta Ala470 (isoform 1 numbering); isoform 3 = delta E135-K146 compared to isoform 1 NCBI ref
• Low Temp: No