cDNA Clone NIK

• Synonyms: MAP3K14
• Plasmid: pBABE Puro NIK Ala25Pro
• Parent Plasmid: pBabe puro
• Genbank: NM_003954.4
• Species: Human

Sequence of Insert

GGATCCGCAGTGATGGAAATGGCCTGCCCAGGTGCCCCTGGCTCAGCAGT
GGGGCAGCAGAAGGAACTCCCCAAGCCAAAGGAGAAGACGCCGCCACTGG
GGAAGAAACAGAGCTCCGTCTACAAGCTTGAGGCCGTGGAGAAGAGCCCT
GTGTTCTGCGGAAAGTGGGAGATCCTGAATGACGTGATTACCAAGGGCAC
AGCCAAGGAAGGCTCCGAGGCAGGGCCAGCTGCCATCTCTATCATCGCCC
AGGCTGAGTGTGAGAATAGCCAAGAGTTCAGCCCCACCTTTTCAGAACGC
ATTTTCATCGCTGGGTCCAAACAGTACAGCCAGTCCGAGAGTCTTGATCA
GATCCCCAACAATGTGGCCCATGCTACAGAGGGCAAAATGGCCCGTGTGT
GTTGGAAGGGAAAGCGTCGCAGCAAAGCCCGGAAGAAACGGAAGAAGAAG
AGCTCAAAGTCCCTGGCTCATGCAGGAGTGGCCTTGGCCAAACCCCTCCC
CAGGACCCCTGAGCAGGAGAGCTGCACCATCCCAGTGCAGGAGGATGAGT
CTCCACTCGGCGCCCCATATGTTAGAAACACCCCGCAGTTCACCAAGCCT
CTGAAGGAACCAGGCCTTGGGCAACTCTGTTTTAAGCAGCTTGGCGAGGG
CCTACGGCCGGCTCTGCCTCGATCAGAACTCCACAAACTGATCAGCCCCT
TGCAATGTCTGAACCACGTGTGGAAACTGCACCACCCCCAGGACGGAGGC
CCCCTGCCCCTGCCCACGCACCCCTTCCCCTATAGCAGACTGCCTCATCC
CTTCCCATTCCACCCTCTCCAGCCCTGGAAACCTCACCCTCTGGAGTCCT
TCCTGGGCAAACTGGCCTGTGTAGACAGCCAGAAACCCTTGCCTGACCCA
CACCTGAGCAAACTGGCCTGTGTAGACAGTCCAAAGCCCCTGCCTGGCCC
ACACCTGGAGCCCAGCTGCCTGTCTCGTGGTGCCCATGAGAAGTTTTCTG
TGGAGGAATACCTAGTGCATGCTCTGCAAGGCAGCGTGAGCTCAGGCCAG
GCCCACAGCCTGACCAGCCTGGCCAAGACCTGGGCAGCAAGGGGCTCCAG
ATCCCGGGAGCCCAGCCCCAAAACTGAGGACAACGAGGGTGTCCTGCTCA
CTGAGAAACTCAAGCCAGTGGATTATGAGTACCGAGAAGAAGTCCACTGG
GCCACGCACCAGCTCCGCCTGGGCAGAGGCTCCTTCGGAGAGGTGCACAG
GATGGAGGACAAGCAGACTGGCTTCCAGTGCGCTGTCAAAAAGGTGCGGC
TGGAAGTATTTCGGGCAGAGGAGCTGATGGCATGTGCAGGATTGACCTCA
CCCAGAATTGTCCCTTTGTATGGAGCTGTGAGAGAAGGGCCTTGGGTCAA
CATCTTCATGGAGCTGCTGGAAGGTGGCTCCCTGGGCCAGCTGGTCAAGG
AGCAGGGCTGTCTCCCAGAGGACCGGGCCCTGTACTACCTGGGCCAGGCC
CTGGAGGGTCTGGAATACCTCCACTCACGAAGGATTCTGCATGGGGACGT
CAAAGCTGACAACGTGCTCCTGTCCAGCGATGGGAGCCACGCAGCCCTCT
GTGACTTTGGCCATGCTGTGTGTCTTCAACCTGATGGCCTGGGAAAGTCC
TTGCTCACAGGGGACTACATCCCTGGCACAGAGACCCACATGGCTCCGGA
GGTGGTGCTGGGCAGGAGCTGCGACGCCAAGGTGGATGTCTGGAGCAGCT
GCTGTATGATGCTGCACATGCTCAACGGCTGCCACCCCTGGACTCAGTTC
TTCCGAGGGCCGCTCTGCCTCAAGATTGCCAGCGAGCCTCCGCCTGTGAG
GGAGATCCCACCCTCCTGCGCCCCTCTCACAGCCCAGGCCATCCAAGAGG
GGCTGAGGAAAGAGCCCATCCACCGCGTGTCTGCAGCGGAGCTGGGAGGG
AAGGTGAACCGGGCACTACAGCAAGTGGGAGGTCTGAAGAGCCCTTGGAG
GGGAGAATATAAAGAACCAAGACATCCACCGCCAAATCAAGCCAATTACC
ACCAGACCCTCCATGCCCAGCCGAGAGAGCTTTCGCCAAGGGCCCCAGGG
CCCCGGCCAGCTGAGGAGACAACAGGCAGAGCCCCTAAGCTCCAGCCTCC
TCTCCCACCAGAGCCCCCAGAGCCAAACAAGTCTCCTCCCTTGACTTTGA
GCAAGGAGGAGTCTGGGATGTGGGAACCCTTACCTCTGTCCTCCCTGGAG
CCAGCCCCTGCCAGAAACCCCAGCTCACCAGAGCGGAAAGCAACCGTCCC
GGAGCAGGAACTGCAGCAGCTGGAAATAGAATTATTCCTCAACAGCCTGT
CCCAGCCATTTTCTCTGGAGGAGCAGGAGCAAATTCTCTCGTGCCTCAGC
ATCGACAGCCTCTCCCTGTCGGATGACAGTGAGAAGAACCCATCAAAGGC
CTCTCAAAGCTCGCGGGACACCCTGAGCTCAGGCGTACACTCCTGGAGCA
GCCAGGCCGAGGCTCGAAGCTCCAGCTGGAACATGGTGCTGGCCCGGGGG
CGGCCCACCGACACCCCAAGCTATTTCAATGGTGTGAAAGTCCAAATACA
GTCTCTTAATGGTGAACACCTGCACATCCGGGAGTTCCACCGGGTCAAAG
TGGGAGACATCGCCACTGGCATCAGCAGCCAGATCCCAGCTGCAGCCTTC
AGCTTGGTGACCAAAGACGGGCAGCCTGTTCGCTACGACATGGAGGTGCC
AGACTCGGGCATCGACCTGCAGTGCACACTGGCCCCTGATGGCAGCTTCG
CCTGGAGCTGGAGGGTCAAGCATGGCCAGCTGGAGAACAGGCCCTAAGAA
TTCGAGGTCGAC

Amino Acid Sequence

AVMEMACPGAPGSAVGQQKELPKPKEKTPPLGKKQSSVYKLEAVEKSPVF
CGKWEILNDVITKGTAKEGSEAGPAAISIIAQAECENSQEFSPTFSERIF
IAGSKQYSQSESLDQIPNNVAHATEGKMARVCWKGKRRSKARKKRKKKSS
KSLAHAGVALAKPLPRTPEQESCTIPVQEDESPLGAPYVRNTPQFTKPLK
EPGLGQLCFKQLGEGLRPALPRSELHKLISPLQCLNHVWKLHHPQDGGPL
PLPTHPFPYSRLPHPFPFHPLQPWKPHPLESFLGKLACVDSQKPLPDPHL
SKLACVDSPKPLPGPHLEPSCLSRGAHEKFSVEEYLVHALQGSVSSGQAH
SLTSLAKTWAARGSRSREPSPKTEDNEGVLLTEKLKPVDYEYREEVHWAT
HQLRLGRGSFGEVHRMEDKQTGFQCAVKKVRLEVFRAEELMACAGLTSPR
IVPLYGAVREGPWVNIFMELLEGGSLGQLVKEQGCLPEDRALYYLGQALE
GLEYLHSRRILHGDVKADNVLLSSDGSHAALCDFGHAVCLQPDGLGKSLL
TGDYIPGTETHMAPEVVLGRSCDAKVDVWSSCCMMLHMLNGCHPWTQFFR
GPLCLKIASEPPPVREIPPSCAPLTAQAIQEGLRKEPIHRVSAAELGGKV
NRALQQVGGLKSPWRGEYKEPRHPPPNQANYHQTLHAQPRELSPRAPGPR
PAEETTGRAPKLQPPLPPEPPEPNKSPPLTLSKEESGMWEPLPLSSLEPA
PARNPSSPERKATVPEQELQQLEIELFLNSLSQPFSLEEQEQILSCLSID
SLSLSDDSEKNPSKASQSSRDTLSSGVHSWSSQAEARSSSWNMVLARGRP
TDTPSYFNGVKVQIQSLNGEHLHIREFHRVKVGDIATGISSQIPAAAFSL
VTKDGQPVRYDMEVPDSGIDLQCTLAPDGSFAWSWRVKHGQLENRP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU29287
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Gly348 restored to match updated NCBI; clone still has Ala25Pro mutation to correct; **Clone lacks initiating methionine**
• Low Temp: No