cDNA Clone MAP4K2

• Synonyms: BL44; GCK; RAB8IP
• Plasmid: pCMV5 FLAG1 MAP4K2 D154A
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG MAP4K2 D154A
• Genbank: NM_004579.4
• Tag: FLAG
• Species: Human

Sequence of Insert

GTCGACGCGCTGCTGCGGGATGTGTCGCTGCAGGACCCGCGGGACCGCTTCGAGCTGCTGCAGCGTGTGGGGGCCGGGACCTATGGCGACGTCTACAAGGCCCGCGACACGGTCACGTCCGAACTGGCCGCCGTGAAGATAGTCAAGCTAGACCCAGGGGACGACATCAGCTCCCTCCAGCAGGAAATCACCATCCTGCGTGAGTGCCGCCACCCCAATGTGGTGGCCTACATTGGCAGCTACCTCAGGAATGACCGCTTGTGGATCTGCATGGAGTTCTGCGGAGGGGGCTCCCTGCAGGAGATTTACCATGCCACTGGGCCCCTGGAGGAGCGGCAGATTGCCTACGTCTGCCGAGAGGCACTGAAGGGGCTCCACCACCTGCATTCTCAGGGGAAGATCCACAGAGACATCAAGGGGGCCAACCTTCTCCTCACTCTCCAGGGAGATGTCAAACTGGCTGCCTTTGGGGTGTCAGGCGAGCTGACAGCGTCTGTGGCCAAGAGGAGGTCTTTCATTGGGACTCCCTACTGGATGGCTCCCGAGGTGGCTGCTGTGGAGCGCAAAGGTGGCTACAATGAGCTATGTGACGTCTGGGCCCTGGGCATCACTGCCATTGAGCTGGGCGAGCTGCAGCCCCCTCTGTTCCACCTGCACCCCATGAGGGCCCTGATGCTCATGTCGAAGAGCAGCTTCCAGCCGCCCAAACTGAGAGATAAGACTCGCTGGACCCAGAATTTCCACCACTTTCTCAAACTGGCCCTGACCAAGAATCCTAAGAAGAGGCCGACAGCAGAGAAGCTCCTGCAGCACCCGTTCACGACTCAGCAGCTCCCTCGGGCCCTCCTCACACAGCTGCTGGACAAAGCCAGTGACCCTCATCTGGGGACCCCCTCCCCTGAGGACTGTGAGCTGGAGACCTATGACATGTTTCCAGACACCATTCACTCCCGGGGGCAGCACGGCCCAGCCGAGAGGACCCCCTCGGAGATCCAGTTTCACCAGGTGAAATTTGGCGCCCCACGCAGGAAGGAAACTGACCCACTGAATGAGCCGTGGGAGGAAGAGTGGACACTACTGGGAAAGGAAGAGTTGAGTGGGAGCCTGCTGCAGTCGGTCCAGGAGGCCCTGGAGGAAAGGAGTCTGACTATTCGGTCAGCCTCAGAATTCCAGGAGCTGGACTCCCCAGACGATACCATGGGAACCATCAAGCGGGCCCCGTTCCTAGGGCCACTCCCCACTGACCCTCCAGCAGAGGAGCCTCTGTCCAGTCCCCCAGGAACCCTGCCCCCACCTCCTTCAGGCCCCAACAGCTCCCCACTGCTGCCCACGGCCTGGGCCACCATGAAGCAGCGGGAGGATCCTGAGAGGTCATCCTGCCACGGGCTCCCCCCAACTCCCAAGGTGCATATGGGCGCCTGCTTCTCCAAGGTCTTCAATGGCTGCCCCCTGCGGATCCACGCTGCTGTCACCTGGATTCACCCTGTTACTCGGGACCAGTTCCTGGTGGTAGGGGCCGAGGAAGGCATCTACACACTCAACCTGCATGAACTGCATGAGGATACGCTGGAGAAGCTGATTTCACATCGCTGCTCCTGGCTCTACTGCGTGAACAACGTGCTGCTGTCACTCTCAGGGAAATCCACGCACATCTGGGCCCATGACCTCCCAGGCCTGTTTGAGCAGCGGAGGCTACAGCAACAGGTTCCCCTCTCCATCCCCACCAACCGCCTCACCCAGCGCATCATCCCCAGGCGCTTTGCTCTGTCCACCAAGATTCCTGACACCAAAGGCTGCTTGCAGTGTCGTGTGGTGCGGAACCCCTACACGGGTGCCACCTTCCTGCTGGCCGCCCTGCCCACCAGCCTGCTCCTGCTGCAGTGGTATGAGCCGCTGCAGAAGTTTCTGCTGCTGAAGAACTTCTCCAGCCCTCTGCCCAGCCCAGCTGGGATGCTGGAGCCGCTGGTGCTGGATGGGAAGGAGCTGCCGCAGGTGTGTGTTGGGGCCGAGGGGCCTGAGGGGCCCGGCTGCCGCGTCCTGTTCCATGTCCTGCCCCTGGAGGCTGGCCTGACGCCCGACATCCTCATCCCACCTGAGGGGATCCCAGGCTCGGCCCAGCAGGTGATCCAGGTGGACAGGGACACAATCCTAGTCAGCTTTGAACGCTGTGTGAGGATTGTCAACATGCAGGGCGAGCCCACGGCCACACTGGCACCTGAGCTGACCTTTGATTTCCCCATCGAGACTGTGGTGTGCCTGCAGGACAGTGTGCTGGCCTTCTGGAGCCATGGGATGCAAGGCCGAAGCCTGGATACCAATGAGGTGACCCAGGAGATCACAGATGAAACAAGGATCTTCCGAGTGCTTGGGGCCCACAGAGACATCATCCTGGAGAGCATTCCCACTGACAACCCAGAGGCGCACAGCAACCTCTACATCCTCACGGGCCACCAGAGCACCTACTAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGDPRNSRVDALLRDVSLQDPRDRFELLQRVGAGTYGDVYKARDTVTSELAAVKIVKLDPGDDISSLQQEITILRECRHPNVVAYIGSYLRN
DRLWICMEFCGGGSLQEIYHATGPLEERQIAYVCREALKGLHHLHSQGKIHRDIKGANLLLTLQGDVKLAAFGVSGELTASVAKRRSFIGTPYWMAPEVA
AVERKGGYNELCDVWALGITAIELGELQPPLFHLHPMRALMLMSKSSFQPPKLRDKTRWTQNFHHFLKLALTKNPKKRPTAEKLLQHPFTTQQLPRALLT
QLLDKASDPHLGTPSPEDCELETYDMFPDTIHSRGQHGPAERTPSEIQFHQVKFGAPRRKETDPLNEPWEEEWTLLGKEELSGSLLQSVQEALEERSLTI
RSASEFQELDSPDDTMGTIKRAPFLGPLPTDPPAEEPLSSPPGTLPPPPSGPNSSPLLPTAWATMKQREDPERSSCHGLPPTPKVHMGACFSKVFNGCPL
RIHAAVTWIHPVTRDQFLVVGAEEGIYTLNLHELHEDTLEKLISHRCSWLYCVNNVLLSLSGKSTHIWAHDLPGLFEQRRLQQQVPLSIPTNRLTQRIIP
RRFALSTKIPDTKGCLQCRVVRNPYTGATFLLAALPTSLLLLQWYEPLQKFLLLKNFSSPLPSPAGMLEPLVLDGKELPQVCVGAEGPEGPGCRVLFHVL
PLEAGLTPDILIPPEGIPGSAQQVIQVDRDTILVSFERCVRIVNMQGEPTATLAPELTFDFPIETVVCLQDSVLAFWSHGMQGRSLDTNEVTQEITDETR
IFRVLGAHRDIILESIPTDNPEAHSNLYILTGHQSTY*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU58725
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Changes M220V and L796R in clone DU56365 back-mutated to wild-type in this clone.
• Low Temp: No