cDNA Clone NIK

• Synonyms: MAP3K14
• Plasmid: pBABE Puro NIK Ala25Pro delta I78-A84
• Parent Plasmid: pBabe puro
• Genbank: NM_003954.4
• Species: Human

Sequence of Insert

GGATCCGCAGTGATGGAAATGGCCTGCCCAGGTGCCCCTGGCTCAGCAGT
GGGGCAGCAGAAGGAACTCCCCAAGCCAAAGGAGAAGACGCCGCCACTGG
GGAAGAAACAGAGCTCCGTCTACAAGCTTGAGGCCGTGGAGAAGAGCCCT
GTGTTCTGCGGAAAGTGGGAGATCCTGAATGACGTGATTACCAAGGGCAC
AGCCAAGGAAGGCTCCGAGGCAGGGCCAGCTGCCGAGTGTGAGAATAGCC
AAGAGTTCAGCCCCACCTTTTCAGAACGCATTTTCATCGCTGGGTCCAAA
CAGTACAGCCAGTCCGAGAGTCTTGATCAGATCCCCAACAATGTGGCCCA
TGCTACAGAGGGCAAAATGGCCCGTGTGTGTTGGAAGGGAAAGCGTCGCA
GCAAAGCCCGGAAGAAACGGAAGAAGAAGAGCTCAAAGTCCCTGGCTCAT
GCAGGAGTGGCCTTGGCCAAACCCCTCCCCAGGACCCCTGAGCAGGAGAG
CTGCACCATCCCAGTGCAGGAGGATGAGTCTCCACTCGGCGCCCCATATG
TTAGAAACACCCCGCAGTTCACCAAGCCTCTGAAGGAACCAGGCCTTGGG
CAACTCTGTTTTAAGCAGCTTGGCGAGGGCCTACGGCCGGCTCTGCCTCG
ATCAGAACTCCACAAACTGATCAGCCCCTTGCAATGTCTGAACCACGTGT
GGAAACTGCACCACCCCCAGGACGGAGGCCCCCTGCCCCTGCCCACGCAC
CCCTTCCCCTATAGCAGACTGCCTCATCCCTTCCCATTCCACCCTCTCCA
GCCCTGGAAACCTCACCCTCTGGAGTCCTTCCTGGGCAAACTGGCCTGTG
TAGACAGCCAGAAACCCTTGCCTGACCCACACCTGAGCAAACTGGCCTGT
GTAGACAGTCCAAAGCCCCTGCCTGGCCCACACCTGGAGCCCAGCTGCCT
GTCTCGTGGTGCCCATGAGAAGTTTTCTGTGGAGGAATACCTAGTGCATG
CTCTGCAAGGCAGCGTGAGCTCAGGCCAGGCCCACAGCCTGACCAGCCTG
GCCAAGACCTGGGCAGCAAGGGGCTCCAGATCCCGGGAGCCCAGCCCCAA
AACTGAGGACAACGAGGGTGTCCTGCTCACTGAGAAACTCAAGCCAGTGG
ATTATGAGTACCGAGAAGAAGTCCACTGGGCCACGCACCAGCTCCGCCTG
GGCAGAGGCTCCTTCGGAGAGGTGCACAGGATGGAGGACAAGCAGACTGG
CTTCCAGTGCGCTGTCAAAAAGGTGCGGCTGGAAGTATTTCGGGCAGAGG
AGCTGATGGCATGTGCAGGATTGACCTCACCCAGAATTGTCCCTTTGTAT
GGAGCTGTGAGAGAAGGGCCTTGGGTCAACATCTTCATGGAGCTGCTGGA
AGGTGGCTCCCTGGGCCAGCTGGTCAAGGAGCAGGGCTGTCTCCCAGAGG
ACCGGGCCCTGTACTACCTGGGCCAGGCCCTGGAGGGTCTGGAATACCTC
CACTCACGAAGGATTCTGCATGGGGACGTCAAAGCTGACAACGTGCTCCT
GTCCAGCGATGGGAGCCACGCAGCCCTCTGTGACTTTGGCCATGCTGTGT
GTCTTCAACCTGATGGCCTGGGAAAGTCCTTGCTCACAGGGGACTACATC
CCTGGCACAGAGACCCACATGGCTCCGGAGGTGGTGCTGGGCAGGAGCTG
CGACGCCAAGGTGGATGTCTGGAGCAGCTGCTGTATGATGCTGCACATGC
TCAACGGCTGCCACCCCTGGACTCAGTTCTTCCGAGGGCCGCTCTGCCTC
AAGATTGCCAGCGAGCCTCCGCCTGTGAGGGAGATCCCACCCTCCTGCGC
CCCTCTCACAGCCCAGGCCATCCAAGAGGGGCTGAGGAAAGAGCCCATCC
ACCGCGTGTCTGCAGCGGAGCTGGGAGGGAAGGTGAACCGGGCACTACAG
CAAGTGGGAGGTCTGAAGAGCCCTTGGAGGGGAGAATATAAAGAACCAAG
ACATCCACCGCCAAATCAAGCCAATTACCACCAGACCCTCCATGCCCAGC
CGAGAGAGCTTTCGCCAAGGGCCCCAGGGCCCCGGCCAGCTGAGGAGACA
ACAGGCAGAGCCCCTAAGCTCCAGCCTCCTCTCCCACCAGAGCCCCCAGA
GCCAAACAAGTCTCCTCCCTTGACTTTGAGCAAGGAGGAGTCTGGGATGT
GGGAACCCTTACCTCTGTCCTCCCTGGAGCCAGCCCCTGCCAGAAACCCC
AGCTCACCAGAGCGGAAAGCAACCGTCCCGGAGCAGGAACTGCAGCAGCT
GGAAATAGAATTATTCCTCAACAGCCTGTCCCAGCCATTTTCTCTGGAGG
AGCAGGAGCAAATTCTCTCGTGCCTCAGCATCGACAGCCTCTCCCTGTCG
GATGACAGTGAGAAGAACCCATCAAAGGCCTCTCAAAGCTCGCGGGACAC
CCTGAGCTCAGGCGTACACTCCTGGAGCAGCCAGGCCGAGGCTCGAAGCT
CCAGCTGGAACATGGTGCTGGCCCGGGGGCGGCCCACCGACACCCCAAGC
TATTTCAATGGTGTGAAAGTCCAAATACAGTCTCTTAATGGTGAACACCT
GCACATCCGGGAGTTCCACCGGGTCAAAGTGGGAGACATCGCCACTGGCA
TCAGCAGCCAGATCCCAGCTGCAGCCTTCAGCTTGGTGACCAAAGACGGG
CAGCCTGTTCGCTACGACATGGAGGTGCCAGACTCGGGCATCGACCTGCA
GTGCACACTGGCCCCTGATGGCAGCTTCGCCTGGAGCTGGAGGGTCAAGC
ATGGCCAGCTGGAGAACAGGCCCTAAGAATTCGAGGTCGAC

Amino Acid Sequence

AVMEMACPGAPGSAVGQQKELPKPKEKTPPLGKKQSSVYKLEAVEKSPVF
CGKWEILNDVITKGTAKEGSEAGPAAECENSQEFSPTFSERIFIAGSKQY
SQSESLDQIPNNVAHATEGKMARVCWKGKRRSKARKKRKKKSSKSLAHAG
VALAKPLPRTPEQESCTIPVQEDESPLGAPYVRNTPQFTKPLKEPGLGQL
CFKQLGEGLRPALPRSELHKLISPLQCLNHVWKLHHPQDGGPLPLPTHPF
PYSRLPHPFPFHPLQPWKPHPLESFLGKLACVDSQKPLPDPHLSKLACVD
SPKPLPGPHLEPSCLSRGAHEKFSVEEYLVHALQGSVSSGQAHSLTSLAK
TWAARGSRSREPSPKTEDNEGVLLTEKLKPVDYEYREEVHWATHQLRLGR
GSFGEVHRMEDKQTGFQCAVKKVRLEVFRAEELMACAGLTSPRIVPLYGA
VREGPWVNIFMELLEGGSLGQLVKEQGCLPEDRALYYLGQALEGLEYLHS
RRILHGDVKADNVLLSSDGSHAALCDFGHAVCLQPDGLGKSLLTGDYIPG
TETHMAPEVVLGRSCDAKVDVWSSCCMMLHMLNGCHPWTQFFRGPLCLKI
ASEPPPVREIPPSCAPLTAQAIQEGLRKEPIHRVSAAELGGKVNRALQQV
GGLKSPWRGEYKEPRHPPPNQANYHQTLHAQPRELSPRAPGPRPAEETTG
RAPKLQPPLPPEPPEPNKSPPLTLSKEESGMWEPLPLSSLEPAPARNPSS
PERKATVPEQELQQLEIELFLNSLSQPFSLEEQEQILSCLSIDSLSLSDD
SEKNPSKASQSSRDTLSSGVHSWSSQAEARSSSWNMVLARGRPTDTPSYF
NGVKVQIQSLNGEHLHIREFHRVKVGDIATGISSQIPAAAFSLVTKDGQP
VRYDMEVPDSGIDLQCTLAPDGSFAWSWRVKHGQLENRP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU29306
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Gly348 restored to match updated NCBI; clone still has Ala25Pro mutation to correct; Silent change at V902 (GTC > GTG); **Clone lacks initiating methionine**
• Low Temp: No