cDNA Clone Dengue virus non-structural protein 3

• Plasmid: pGEX6P-1-Dengue Virus 2 (aa1476 - 2093)
• Parent Plasmid: pGEX6P-1
• Expressed: Get-Dengue Virus 2 (aa1476 - 2093)
• Genbank: AAF18446.1
• Tag: GST

Sequence of Insert

GGATCCGCAGGCGTTCTGTGGGATGTTCCGAGTCCGCCTCCGGTTGGTAA
AGCAGAACTGGAAGATGGTGCATATCGCATTAAACAGAAAGGTATTCTGG
GTTATAGCCAGATTGGTGCCGGTGTTTATAAAGAAGGCACCTTTCATACC
ATGTGGCATGTTACCCGTGGTGCAGTTCTGATGCATAAAGGTAAACGTAT
TGAACCGAGCTGGGCAGATGTTAAAAAAGATCTGATTAGCTATGCCGGTG
GTTGGAAACTGGAAGGTGAATGGAAAGAAGGTGAAGAAGTTCAGGTTCTG
GCACTGGAACCGGGTAAAAATCCGCGTGCAGTTCAGACCAAACCGGGTCT
GTTTAAAACCAATGCAGGCACCATTGGTGCGGTTAGCCTGGATTTTAGTC
CGGGTACAAGCGGTAGCCCGATTATTGATAAAAAAGGTAAAGTGGTGGGC
CTGTATGGTAATGGTGTTGTGACCCGTAGCGGTGCATATGTTAGCGCAAT
TGCACAGACCGAAAAAAGCATTGAAGATAATCCGGAAATCGAGGATGATA
TCTTTCGTAAACGTAAGCTGACCATCATGGATCTGCATCCTGGTGCAGGT
AAAACCAAACGTTATCTGCCTGCAATTGTTCGCGAAGCCATTAAACGTGG
TCTGCGTACCCTGATTCTGGCACCGACACGTGTTGTTGCAGCCGAAATGG
AAGAGGCACTGCGTGGCCTGCCGATTCGTTATCAGACACCGGCAATTCGT
GCAGAACATACCGGTCGTGAAATTGTTGATCTGATGTGTCATGCAACCTT
TACCATGCGTCTGCTGAGTCCGGTTCGTGTTCCGAATTATAACCTGATTA
TTATGGATGAAGCCCACTTTACAGATCCGGCAAGCATTGCAGCACGTGGT
TATATTTCAACCCGTGTGGAAATGGGTGAAGCAGCAGGCATTTTTATGAC
CGCAACACCGCCTGGTAGCCGTGATCCGTTTCCGCAGAGCAATGCACCGA
TTATGGACGAAGAACGTGAAATTCCGGAACGTAGCTGGTCAAGCGGTCAT
GAATGGGTTACCGATTTTAAAGGCAAAACCGTGTGGTTTGTTCCGAGCAT
TAAAGCCGGTAATGATATTGCAGCATGCCTGCGTAAAAATGGCAAAAAAG
TTATTCAGCTGAGCCGCAAAACCTTCGATAGCGAATATGTTAAAACCCGT
ACCAACGATTGGGATTTTGTTGTTACCACCGATATTAGCGAAATGGGAGC
AAACTTTAAAGCCGAACGTGTTATTGATCCGCGTCGTTGTATGAAACCGG
TTATTCTGACCGATGGTGAAGAACGCGTTATTCTGGCAGGTCCGATGCCG
GTTACACATAGCAGCGCAGCACAGCGTCGTGGTCGTATTGGTCGTAATCC
GAAAAATGAAAACGACCAGTATATCTATATGGGCGAACCGCTGGAAAATG
ATGAAGATTGTGCACATTGGAAAGAGGCCAAAATGCTGCTGGATAATATC
AATACACCGGAAGGTATTATCCCGAGCATGTTTGAACCGGAACGCGAAAA
AGTTGATGCAATTGATGGCGAATATCGTCTGCGTGGTGAAGCACGTAAAA
CCTTTGTGGACCTGATGCGTCGCGGTGATCTGCCGGTTTGGCTGGCATAT
CGTGTTGCAGCGGAAGGCATTAACTATGCAGATCGTCGTTGGTGTTTTGA
CGGCATTAAAAACAATCAGATCCTGGAAGAAAACGTGGAAGTTGAAATTT
GGACCAAAGAAGGCGAACGCAAAAAACTGAAACCGCGTTGGCTGGATGCA
CGTATTTATAGCGATCCGCTGGCACTGAAAGAATTCAAAGAATTTGCAGC
CGGTCGCAAATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAGVLWDVPSPPPVGKAELE
DGAYRIKQKGILGYSQIGAGVYKEGTFHTMWHVTRGAVLMHKGKRIEPSW
ADVKKDLISYAGGWKLEGEWKEGEEVQVLALEPGKNPRAVQTKPGLFKTN
AGTIGAVSLDFSPGTSGSPIIDKKGKVVGLYGNGVVTRSGAYVSAIAQTE
KSIEDNPEIEDDIFRKRKLTIMDLHPGAGKTKRYLPAIVREAIKRGLRTL
ILAPTRVVAAEMEEALRGLPIRYQTPAIRAEHTGREIVDLMCHATFTMRL
LSPVRVPNYNLIIMDEAHFTDPASIAARGYISTRVEMGEAAGIFMTATPP
GSRDPFPQSNAPIMDEEREIPERSWSSGHEWVTDFKGKTVWFVPSIKAGN
DIAACLRKNGKKVIQLSRKTFDSEYVKTRTNDWDFVVTTDISEMGANFKA
ERVIDPRRCMKPVILTDGEERVILAGPMPVTHSSAAQRRGRIGRNPKNEN
DQYIYMGEPLENDEDCAHWKEAKMLLDNINTPEGIIPSMFEPEREKVDAI
DGEYRLRGEARKTFVDLMRRGDLPVWLAYRVAAEGINYADRRWCFDGIKN
NQILEENVEVEIWTKEGERKKLKPRWLDARIYSDPLALKEFKEFAAGRK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU29309
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: amino acids 1476 - 2093 of AAF18446.1 polyprotein [Dengue virus 2] ; Codon optimised for expression in E. coli
• Low Temp: No