cDNA Clone MAP4K2 (human)

• Plasmid: pFastBac-6His-TEV-HALO-C3-MAP4K2
• Parent Plasmid: pFastBac-6His-TEV-HALO-C3
• Expressed: 6His-HALO-MAP4K2
• Genbank: NM_004579.4
• Tag: 6His
• Tag: HALO
• Species: Human

Sequence of Insert

agatctGCGCTGCTGCGGGATGTGTCGCTGCAGGACCCGCGGGACCGCTT
CGAGCTGCTGCAGCGTGTGGGGGCCGGGACCTATGGCGACGTCTACAAGG
CCCGCGACACGGTCACGTCCGAACTGGCCGCCGTGAAGATAGTCAAGCTA
GACCCAGGGGACGACATCAGCTCCCTCCAGCAGGAAATCACCATCCTGCG
TGAGTGCCGCCACCCCAATGTGGTGGCCTACATTGGCAGCTACCTCAGGA
ATGACCGCTTGTGGATCTGCATGGAGTTCTGCGGAGGGGGCTCCCTGCAG
GAGATTTACCATGCCACTGGGCCCCTGGAGGAGCGGCAGATTGCCTACGT
CTGCCGAGAGGCACTGAAGGGGCTCCACCACCTGCATTCTCAGGGGAAGA
TCCACAGAGACATCAAGGGGGCCAACCTTCTCCTCACTCTCCAGGGAGAT
GTCAAACTGGCTGACTTTGGGGTGTCAGGCGAGCTGACAGCGTCTGTGGC
CAAGAGGAGGTCTTTCATTGGGACTCCCTACTGGATGGCTCCCGAGGTGG
CTGCTGTGGAGCGCAAAGGTGGCTACAATGAGCTATGTGACGTCTGGGCC
CTGGGCATCACTGCCATTGAGCTGGGCGAGCTGCAGCCCCCTCTGTTCCA
CCTGCACCCCATGAGGGCCCTGATGCTCATGTCGAAGAGCAGCTTCCAGC
CGCCCAAACTGAGAGATAAGACTCGCTGGACCCAGAATTTCCACCACTTT
CTCAAACTGGCCCTGACCAAGAATCCTAAGAAGAGGCCGACAGCAGAGAA
GCTCCTGCAGCACCCGTTCACGACTCAGCAGCTCCCTCGGGCCCTCCTCA
CACAGCTGCTGGACAAAGCCAGTGACCCTCATCTGGGGACCCCCTCCCCT
GAGGACTGTGAGCTGGAGACCTATGACATGTTTCCAGACACCATTCACTC
CCGGGGGCAGCACGGCCCAGCCGAGAGGACCCCCTCGGAGATCCAGTTTC
ACCAGGTGAAATTTGGCGCCCCACGCAGGAAGGAAACTGACCCACTGAAT
GAGCCGTGGGAGGAAGAGTGGACACTACTGGGAAAGGAAGAGTTGAGTGG
GAGCCTGCTGCAGTCGGTCCAGGAGGCCCTGGAGGAAAGGAGTCTGACTA
TTCGGTCAGCCTCAGAATTCCAGGAGCTGGACTCCCCAGACGATACCATG
GGAACCATCAAGCGGGCCCCGTTCCTAGGGCCACTCCCCACTGACCCTCC
AGCAGAGGAGCCTCTGTCCAGTCCCCCAGGAACCCTGCCCCCACCTCCTT
CAGGCCCCAACAGCTCCCCACTGCTGCCCACGGCCTGGGCCACCATGAAG
CAGCGGGAGGATCCTGAGAGGTCATCCTGCCACGGGCTCCCCCCAACTCC
CAAGGTGCATATGGGCGCCTGCTTCTCCAAGGTCTTCAATGGCTGCCCCC
TGCGGATCCACGCTGCTGTCACCTGGATTCACCCTGTTACTCGGGACCAG
TTCCTGGTGGTAGGGGCCGAGGAAGGCATCTACACACTCAACCTGCATGA
ACTGCATGAGGATACGCTGGAGAAGCTGATTTCACATCGCTGCTCCTGGC
TCTACTGCGTGAACAACGTGCTGCTGTCACTCTCAGGGAAATCCACGCAC
ATCTGGGCCCATGACCTCCCAGGCCTGTTTGAGCAGCGGAGGCTACAGCA
ACAGGTTCCCCTCTCCATCCCCACCAACCGCCTCACCCAGCGCATCATCC
CCAGGCGCTTTGCTCTGTCCACCAAGATTCCTGACACCAAAGGCTGCTTG
CAGTGTCGTGTGGTGCGGAACCCCTACACGGGTGCCACCTTCCTGCTGGC
CGCCCTGCCCACCAGCCTGCTCCTGCTGCAGTGGTATGAGCCGCTGCAGA
AGTTTCTGCTGCTGAAGAACTTCTCCAGCCCTCTGCCCAGCCCAGCTGGG
ATGCTGGAGCCGCTGGTGCTGGATGGGAAGGAGCTGCCGCAGGTGTGTGT
TGGGGCCGAGGGGCCTGAGGGGCCCGGCTGCCGCGTCCTGTTCCATGTCC
TGCCCCTGGAGGCTGGCCTGACGCCCGACATCCTCATCCCACCTGAGGGG
ATCCCAGGCTCGGCCCAGCAGGTGATCCAGGTGGACAGGGACACAATCCT
AGTCAGCTTTGAACGCTGTGTGAGGATTGTCAACATGCAGGGCGAGCCCA
CGGCCACACTGGCACCTGAGCTGACCTTTGATTTCCCCATCGAGACTGTG
GTGTGCCTGCAGGACAGTGTGCTGGCCTTCTGGAGCCATGGGATGCAAGG
CCGAAGCCTGGATACCAATGAGGTGACCCAGGAGATCACAGATGAAACAA
GGATCTTCCGAGTGCTTGGGGCCCACAGAGACATCATCCTGGAGAGCATT
CCCACTGACAACCCAGAGGCGCACAGCAACCTCTACATCCTCACGGGCCA
CCAGAGCACCTACTAAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMAEIGTGFPFDPHYVEVLGERMHYV
DVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKP
DLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKG
IAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPM
GVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEY
MDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQ
EDNPDLIGSEIARWLSTLEISGLEVLFQGPRSALLRDVSLQDPRDRFELL
QRVGAGTYGDVYKARDTVTSELAAVKIVKLDPGDDISSLQQEITILRECR
HPNVVAYIGSYLRNDRLWICMEFCGGGSLQEIYHATGPLEERQIAYVCRE
ALKGLHHLHSQGKIHRDIKGANLLLTLQGDVKLADFGVSGELTASVAKRR
SFIGTPYWMAPEVAAVERKGGYNELCDVWALGITAIELGELQPPLFHLHP
MRALMLMSKSSFQPPKLRDKTRWTQNFHHFLKLALTKNPKKRPTAEKLLQ
HPFTTQQLPRALLTQLLDKASDPHLGTPSPEDCELETYDMFPDTIHSRGQ
HGPAERTPSEIQFHQVKFGAPRRKETDPLNEPWEEEWTLLGKEELSGSLL
QSVQEALEERSLTIRSASEFQELDSPDDTMGTIKRAPFLGPLPTDPPAEE
PLSSPPGTLPPPPSGPNSSPLLPTAWATMKQREDPERSSCHGLPPTPKVH
MGACFSKVFNGCPLRIHAAVTWIHPVTRDQFLVVGAEEGIYTLNLHELHE
DTLEKLISHRCSWLYCVNNVLLSLSGKSTHIWAHDLPGLFEQRRLQQQVP
LSIPTNRLTQRIIPRRFALSTKIPDTKGCLQCRVVRNPYTGATFLLAALP
TSLLLLQWYEPLQKFLLLKNFSSPLPSPAGMLEPLVLDGKELPQVCVGAE
GPEGPGCRVLFHVLPLEAGLTPDILIPPEGIPGSAQQVIQVDRDTILVSF
ERCVRIVNMQGEPTATLAPELTFDFPIETVVCLQDSVLAFWSHGMQGRSL
DTNEVTQEITDETRIFRVLGAHRDIILESIPTDNPEAHSNLYILTGHQST
Y*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU29331
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Full-length isoform 1; Parental 29295
• Low Temp: No