cDNA Clone NIK

• Synonyms: MAP3K14
• Plasmid: pBABE Puro NIK delta I78-A84
• Parent Plasmid: pBabe puro
• Expressed: NIK delta I78-A84
• Genbank: NM_003954.4
• Species: Human

Sequence of Insert

GGATCCATGGCAGTGATGGAAATGGCCTGCCCAGGTGCCCCTGGCTCAGCAGT
GGGGCAGCAGAAGGAACTCCCCAAAGCCAAGGAGAAGACGCCGCCACTGG
GGAAGAAACAGAGCTCCGTCTACAAGCTTGAGGCCGTGGAGAAGAGCCCT
GTGTTCTGCGGAAAGTGGGAGATCCTGAATGACGTGATTACCAAGGGCAC
AGCCAAGGAAGGCTCCGAGGCAGGGCCAGCTGCCGAGTGTGAGAATAGCC
AAGAGTTCAGCCCCACCTTTTCAGAACGCATTTTCATCGCTGGGTCCAAA
CAGTACAGCCAGTCCGAGAGTCTTGATCAGATCCCCAACAATGTGGCCCA
TGCTACAGAGGGCAAAATGGCCCGTGTGTGTTGGAAGGGAAAGCGTCGCA
GCAAAGCCCGGAAGAAACGGAAGAAGAAGAGCTCAAAGTCCCTGGCTCAT
GCAGGAGTGGCCTTGGCCAAACCCCTCCCCAGGACCCCTGAGCAGGAGAG
CTGCACCATCCCAGTGCAGGAGGATGAGTCTCCACTCGGCGCCCCATATG
TTAGAAACACCCCGCAGTTCACCAAGCCTCTGAAGGAACCAGGCCTTGGG
CAACTCTGTTTTAAGCAGCTTGGCGAGGGCCTACGGCCGGCTCTGCCTCG
ATCAGAACTCCACAAACTGATCAGCCCCTTGCAATGTCTGAACCACGTGT
GGAAACTGCACCACCCCCAGGACGGAGGCCCCCTGCCCCTGCCCACGCAC
CCCTTCCCCTATAGCAGACTGCCTCATCCCTTCCCATTCCACCCTCTCCA
GCCCTGGAAACCTCACCCTCTGGAGTCCTTCCTGGGCAAACTGGCCTGTG
TAGACAGCCAGAAACCCTTGCCTGACCCACACCTGAGCAAACTGGCCTGT
GTAGACAGTCCAAAGCCCCTGCCTGGCCCACACCTGGAGCCCAGCTGCCT
GTCTCGTGGTGCCCATGAGAAGTTTTCTGTGGAGGAATACCTAGTGCATG
CTCTGCAAGGCAGCGTGAGCTCAGGCCAGGCCCACAGCCTGACCAGCCTG
GCCAAGACCTGGGCAGCAAGGGGCTCCAGATCCCGGGAGCCCAGCCCCAA
AACTGAGGACAACGAGGGTGTCCTGCTCACTGAGAAACTCAAGCCAGTGG
ATTATGAGTACCGAGAAGAAGTCCACTGGGCCACGCACCAGCTCCGCCTG
GGCAGAGGCTCCTTCGGAGAGGTGCACAGGATGGAGGACAAGCAGACTGG
CTTCCAGTGCGCTGTCAAAAAGGTGCGGCTGGAAGTATTTCGGGCAGAGG
AGCTGATGGCATGTGCAGGATTGACCTCACCCAGAATTGTCCCTTTGTAT
GGAGCTGTGAGAGAAGGGCCTTGGGTCAACATCTTCATGGAGCTGCTGGA
AGGTGGCTCCCTGGGCCAGCTGGTCAAGGAGCAGGGCTGTCTCCCAGAGG
ACCGGGCCCTGTACTACCTGGGCCAGGCCCTGGAGGGTCTGGAATACCTC
CACTCACGAAGGATTCTGCATGGGGACGTCAAAGCTGACAACGTGCTCCT
GTCCAGCGATGGGAGCCACGCAGCCCTCTGTGACTTTGGCCATGCTGTGT
GTCTTCAACCTGATGGCCTGGGAAAGTCCTTGCTCACAGGGGACTACATC
CCTGGCACAGAGACCCACATGGCTCCGGAGGTGGTGCTGGGCAGGAGCTG
CGACGCCAAGGTGGATGTCTGGAGCAGCTGCTGTATGATGCTGCACATGC
TCAACGGCTGCCACCCCTGGACTCAGTTCTTCCGAGGGCCGCTCTGCCTC
AAGATTGCCAGCGAGCCTCCGCCTGTGAGGGAGATCCCACCCTCCTGCGC
CCCTCTCACAGCCCAGGCCATCCAAGAGGGGCTGAGGAAAGAGCCCATCC
ACCGCGTGTCTGCAGCGGAGCTGGGAGGGAAGGTGAACCGGGCACTACAG
CAAGTGGGAGGTCTGAAGAGCCCTTGGAGGGGAGAATATAAAGAACCAAG
ACATCCACCGCCAAATCAAGCCAATTACCACCAGACCCTCCATGCCCAGC
CGAGAGAGCTTTCGCCAAGGGCCCCAGGGCCCCGGCCAGCTGAGGAGACA
ACAGGCAGAGCCCCTAAGCTCCAGCCTCCTCTCCCACCAGAGCCCCCAGA
GCCAAACAAGTCTCCTCCCTTGACTTTGAGCAAGGAGGAGTCTGGGATGT
GGGAACCCTTACCTCTGTCCTCCCTGGAGCCAGCCCCTGCCAGAAACCCC
AGCTCACCAGAGCGGAAAGCAACCGTCCCGGAGCAGGAACTGCAGCAGCT
GGAAATAGAATTATTCCTCAACAGCCTGTCCCAGCCATTTTCTCTGGAGG
AGCAGGAGCAAATTCTCTCGTGCCTCAGCATCGACAGCCTCTCCCTGTCG
GATGACAGTGAGAAGAACCCATCAAAGGCCTCTCAAAGCTCGCGGGACAC
CCTGAGCTCAGGCGTACACTCCTGGAGCAGCCAGGCCGAGGCTCGAAGCT
CCAGCTGGAACATGGTGCTGGCCCGGGGGCGGCCCACCGACACCCCAAGC
TATTTCAATGGTGTGAAAGTCCAAATACAGTCTCTTAATGGTGAACACCT
GCACATCCGGGAGTTCCACCGGGTCAAAGTGGGAGACATCGCCACTGGCA
TCAGCAGCCAGATCCCAGCTGCAGCCTTCAGCTTGGTGACCAAAGACGGG
CAGCCTGTTCGCTACGACATGGAGGTGCCAGACTCGGGCATCGACCTGCA
GTGCACACTGGCCCCTGATGGCAGCTTCGCCTGGAGCTGGAGGGTCAAGC
ATGGCCAGCTGGAGAACAGGCCCTAAGAATTCGAGGTCGAC

Amino Acid Sequence

MAVMEMACPGAPGSAVGQQKELPKAKEKTPPLGKKQSSVYKLEAVEKSPV
FCGKWEILNDVITKGTAKEGSEAGPAAECENSQEFSPTFSERIFIAGSKQ
YSQSESLDQIPNNVAHATEGKMARVCWKGKRRSKARKKRKKKSSKSLAHA
GVALAKPLPRTPEQESCTIPVQEDESPLGAPYVRNTPQFTKPLKEPGLGQ
LCFKQLGEGLRPALPRSELHKLISPLQCLNHVWKLHHPQDGGPLPLPTHP
FPYSRLPHPFPFHPLQPWKPHPLESFLGKLACVDSQKPLPDPHLSKLACV
DSPKPLPGPHLEPSCLSRGAHEKFSVEEYLVHALQGSVSSGQAHSLTSLA
KTWAARGSRSREPSPKTEDNEGVLLTEKLKPVDYEYREEVHWATHQLRLG
RGSFGEVHRMEDKQTGFQCAVKKVRLEVFRAEELMACAGLTSPRIVPLYG
AVREGPWVNIFMELLEGGSLGQLVKEQGCLPEDRALYYLGQALEGLEYLH
SRRILHGDVKADNVLLSSDGSHAALCDFGHAVCLQPDGLGKSLLTGDYIP
GTETHMAPEVVLGRSCDAKVDVWSSCCMMLHMLNGCHPWTQFFRGPLCLK
IASEPPPVREIPPSCAPLTAQAIQEGLRKEPIHRVSAAELGGKVNRALQQ
VGGLKSPWRGEYKEPRHPPPNQANYHQTLHAQPRELSPRAPGPRPAEETT
GRAPKLQPPLPPEPPEPNKSPPLTLSKEESGMWEPLPLSSLEPAPARNPS
SPERKATVPEQELQQLEIELFLNSLSQPFSLEEQEQILSCLSIDSLSLSD
DSEKNPSKASQSSRDTLSSGVHSWSSQAEARSSSWNMVLARGRPTDTPSY
FNGVKVQIQSLNGEHLHIREFHRVKVGDIATGISSQIPAAAFSLVTKDGQ
PVRYDMEVPDSGIDLQCTLAPDGSFAWSWRVKHGQLENRP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU29337
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Gly348 and Ala25 restored to match updated NCBI; Silent change at V902 (GTC > GTG)
• Low Temp: No