cDNA Clone NIK

• Synonyms: MAP3K14
• Plasmid: pBABE Puro NIK delta I78-A84 K429A
• Parent Plasmid: pBabe puro
• Expressed: NIK delta I78-A84 K429A
• Genbank: NM_003954.4
• Species: Human

Sequence of Insert

GGATCCATGGCAGTGATGGAAATGGCCTGCCCAGGTGCCCCTGGCTCAGC
AGTGGGGCAGCAGAAGGAACTCCCCAAAGCCAAGGAGAAGACGCCGCCAC
TGGGGAAGAAACAGAGCTCCGTCTACAAGCTTGAGGCCGTGGAGAAGAGC
CCTGTGTTCTGCGGAAAGTGGGAGATCCTGAATGACGTGATTACCAAGGG
CACAGCCAAGGAAGGCTCCGAGGCAGGGCCAGCTGCCGAGTGTGAGAATA
GCCAAGAGTTCAGCCCCACCTTTTCAGAACGCATTTTCATCGCTGGGTCC
AAACAGTACAGCCAGTCCGAGAGTCTTGATCAGATCCCCAACAATGTGGC
CCATGCTACAGAGGGCAAAATGGCCCGTGTGTGTTGGAAGGGAAAGCGTC
GCAGCAAAGCCCGGAAGAAACGGAAGAAGAAGAGCTCAAAGTCCCTGGCT
CATGCAGGAGTGGCCTTGGCCAAACCCCTCCCCAGGACCCCTGAGCAGGA
GAGCTGCACCATCCCAGTGCAGGAGGATGAGTCTCCACTCGGCGCCCCAT
ATGTTAGAAACACCCCGCAGTTCACCAAGCCTCTGAAGGAACCAGGCCTT
GGGCAACTCTGTTTTAAGCAGCTTGGCGAGGGCCTACGGCCGGCTCTGCC
TCGATCAGAACTCCACAAACTGATCAGCCCCTTGCAATGTCTGAACCACG
TGTGGAAACTGCACCACCCCCAGGACGGAGGCCCCCTGCCCCTGCCCACG
CACCCCTTCCCCTATAGCAGACTGCCTCATCCCTTCCCATTCCACCCTCT
CCAGCCCTGGAAACCTCACCCTCTGGAGTCCTTCCTGGGCAAACTGGCCT
GTGTAGACAGCCAGAAACCCTTGCCTGACCCACACCTGAGCAAACTGGCC
TGTGTAGACAGTCCAAAGCCCCTGCCTGGCCCACACCTGGAGCCCAGCTG
CCTGTCTCGTGGTGCCCATGAGAAGTTTTCTGTGGAGGAATACCTAGTGC
ATGCTCTGCAAGGCAGCGTGAGCTCAGGCCAGGCCCACAGCCTGACCAGC
CTGGCCAAGACCTGGGCAGCAAGGGGCTCCAGATCCCGGGAGCCCAGCCC
CAAAACTGAGGACAACGAGGGTGTCCTGCTCACTGAGAAACTCAAGCCAG
TGGATTATGAGTACCGAGAAGAAGTCCACTGGGCCACGCACCAGCTCCGC
CTGGGCAGAGGCTCCTTCGGAGAGGTGCACAGGATGGAGGACAAGCAGAC
TGGCTTCCAGTGCGCTGTCGCAAAGGTGCGGCTGGAAGTATTTCGGGCAG
AGGAGCTGATGGCATGTGCAGGATTGACCTCACCCAGAATTGTCCCTTTG
TATGGAGCTGTGAGAGAAGGGCCTTGGGTCAACATCTTCATGGAGCTGCT
GGAAGGTGGCTCCCTGGGCCAGCTGGTCAAGGAGCAGGGCTGTCTCCCAG
AGGACCGGGCCCTGTACTACCTGGGCCAGGCCCTGGAGGGTCTGGAATAC
CTCCACTCACGAAGGATTCTGCATGGGGACGTCAAAGCTGACAACGTGCT
CCTGTCCAGCGATGGGAGCCACGCAGCCCTCTGTGACTTTGGCCATGCTG
TGTGTCTTCAACCTGATGGCCTGGGAAAGTCCTTGCTCACAGGGGACTAC
ATCCCTGGCACAGAGACCCACATGGCTCCGGAGGTGGTGCTGGGCAGGAG
CTGCGACGCCAAGGTGGATGTCTGGAGCAGCTGCTGTATGATGCTGCACA
TGCTCAACGGCTGCCACCCCTGGACTCAGTTCTTCCGAGGGCCGCTCTGC
CTCAAGATTGCCAGCGAGCCTCCGCCTGTGAGGGAGATCCCACCCTCCTG
CGCCCCTCTCACAGCCCAGGCCATCCAAGAGGGGCTGAGGAAAGAGCCCA
TCCACCGCGTGTCTGCAGCGGAGCTGGGAGGGAAGGTGAACCGGGCACTA
CAGCAAGTGGGAGGTCTGAAGAGCCCTTGGAGGGGAGAATATAAAGAACC
AAGACATCCACCGCCAAATCAAGCCAATTACCACCAGACCCTCCATGCCC
AGCCGAGAGAGCTTTCGCCAAGGGCCCCAGGGCCCCGGCCAGCTGAGGAG
ACAACAGGCAGAGCCCCTAAGCTCCAGCCTCCTCTCCCACCAGAGCCCCC
AGAGCCAAACAAGTCTCCTCCCTTGACTTTGAGCAAGGAGGAGTCTGGGA
TGTGGGAACCCTTACCTCTGTCCTCCCTGGAGCCAGCCCCTGCCAGAAAC
CCCAGCTCACCAGAGCGGAAAGCAACCGTCCCGGAGCAGGAACTGCAGCA
GCTGGAAATAGAATTATTCCTCAACAGCCTGTCCCAGCCATTTTCTCTGG
AGGAGCAGGAGCAAATTCTCTCGTGCCTCAGCATCGACAGCCTCTCCCTG
TCGGATGACAGTGAGAAGAACCCATCAAAGGCCTCTCAAAGCTCGCGGGA
CACCCTGAGCTCAGGCGTACACTCCTGGAGCAGCCAGGCCGAGGCTCGAA
GCTCCAGCTGGAACATGGTGCTGGCCCGGGGGCGGCCCACCGACACCCCA
AGCTATTTCAATGGTGTGAAAGTCCAAATACAGTCTCTTAATGGTGAACA
CCTGCACATCCGGGAGTTCCACCGGGTCAAAGTGGGAGACATCGCCACTG
GCATCAGCAGCCAGATCCCAGCTGCAGCCTTCAGCTTGGTGACCAAAGAC
GGGCAGCCTGTTCGCTACGACATGGAGGTGCCAGACTCGGGCATCGACCT
GCAGTGCACACTGGCCCCTGATGGCAGCTTCGCCTGGAGCTGGAGGGTCA
AGCATGGCCAGCTGGAGAACAGGCCCTAAGAATTCGAGGTCGAC

Amino Acid Sequence

MAVMEMACPGAPGSAVGQQKELPKAKEKTPPLGKKQSSVYKLEAVEKSPV
FCGKWEILNDVITKGTAKEGSEAGPAAECENSQEFSPTFSERIFIAGSKQ
YSQSESLDQIPNNVAHATEGKMARVCWKGKRRSKARKKRKKKSSKSLAHA
GVALAKPLPRTPEQESCTIPVQEDESPLGAPYVRNTPQFTKPLKEPGLGQ
LCFKQLGEGLRPALPRSELHKLISPLQCLNHVWKLHHPQDGGPLPLPTHP
FPYSRLPHPFPFHPLQPWKPHPLESFLGKLACVDSQKPLPDPHLSKLACV
DSPKPLPGPHLEPSCLSRGAHEKFSVEEYLVHALQGSVSSGQAHSLTSLA
KTWAARGSRSREPSPKTEDNEGVLLTEKLKPVDYEYREEVHWATHQLRLG
RGSFGEVHRMEDKQTGFQCAVAKVRLEVFRAEELMACAGLTSPRIVPLYG
AVREGPWVNIFMELLEGGSLGQLVKEQGCLPEDRALYYLGQALEGLEYLH
SRRILHGDVKADNVLLSSDGSHAALCDFGHAVCLQPDGLGKSLLTGDYIP
GTETHMAPEVVLGRSCDAKVDVWSSCCMMLHMLNGCHPWTQFFRGPLCLK
IASEPPPVREIPPSCAPLTAQAIQEGLRKEPIHRVSAAELGGKVNRALQQ
VGGLKSPWRGEYKEPRHPPPNQANYHQTLHAQPRELSPRAPGPRPAEETT
GRAPKLQPPLPPEPPEPNKSPPLTLSKEESGMWEPLPLSSLEPAPARNPS
SPERKATVPEQELQQLEIELFLNSLSQPFSLEEQEQILSCLSIDSLSLSD
DSEKNPSKASQSSRDTLSSGVHSWSSQAEARSSSWNMVLARGRPTDTPSY
FNGVKVQIQSLNGEHLHIREFHRVKVGDIATGISSQIPAAAFSLVTKDGQ
PVRYDMEVPDSGIDLQCTLAPDGSFAWSWRVKHGQLENRP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU29411
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Gly348 and Ala25 restored to match updated NCBI; Silent change at V902 (GTC > GTG)
• Low Temp: No