cDNA Clone UBE2Z

• Synonyms: ubiquitin-conjugating enzyme E2Z (UBE2Z), Use1
• Plasmid: pET15b 6HIS C3 UBE2Z C100S C261S
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His-UBE2Z C100S C261S
• Genbank: NM_023079.3 GI:150417998*
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGCGGAGAGTCCGACTGAGGAGGCGGCAACGGCGGGCGCCGG
GGCGGCGGGCCCCGGGGCGAGCAGCGTTGCTGGTGTTGTTGGCGTTAGCG
GCAGCGGCGGCGGGTTCGGGCCGCCTTTCCTGCCGGATGTGTGGGCGGCG
GCGGCGGCAGCGGGCGGGGCCGGGGGCCCGGGGAGCGGCCTGGCTCCGCT
GCCCGGGCTCCCGCCCTCAGCCGCTGCCCACGGGGCCGCGCTGCTTAGCC
ACTGGGACCCCACGCTCAGCTCCGACTGGGACGGCGAGCGCACCGCGCCG
CAGTcTCTACTCCGGATCAAGCGGGATATCATGTCCATTTATAAGGAGCC
TCCTCCAGGAATGTTCGTTGTACCTGATACTGTTGACATGACTAAGATTC
ATGCATTGATCACAGGCCCATTTGACACTCCTTATGAAGGGGGTTTCTTC
CTGTTCGTGTTTCGGTGTCCGCCCGACTATCCCATCCACCCACCTCGGGT
CAAACTGATGACAACGGGCAATAACACAGTGAGGTTTAACCCCAACTTCT
ACCGCAATGGGAAAGTCTGCTTGAGTATTCTAGGTACATGGACTGGACCT
GCCTGGAGCCCAGCCCAGAGCATCTCCTCAGTGCTCATCTCTATCCAGTC
CCTGATGACTGAGAACCCCTATCACAATGAGCCCGGCTTTGAACAGGAGA
GACATCCAGGAGACAGCAAAAACTATAATGAATGTATCCGGCACGAGACC
ATCAGAGTTGCAGTCTGTGACATGATGGAAGGAAAGTcTCCCTGTCCTGA
ACCCCTACGAGGGGTGATGGAGAAGTCCTTTCTGGAGTATTACGACTTCT
ATGAGGTGGCCTGCAAAGATCGCCTGCACCTTCAAGGCCAAACTATGCAG
GACCCTTTTGGAGAGAAGCGGGGCCACTTTGACTACCAGTCCCTCTTGAT
GCGCCTGGGACTGATACGTCAGAAAGTGCTGGAGAGGCTCCATAATGAGA
ATGCAGAAATGGACTCTGATAGCAGTTCATCTGGGACAGAGACAGACCTT
CATGGGAGCCTGAGGGTTTAGGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMAESPTEEAATAGAGAAGPGASSVAGV
VGVSGSGGGFGPPFLPDVWAAAAAAGGAGGPGSGLAPLPGLPPSAAAHGA
ALLSHWDPTLSSDWDGERTAPQSLLRIKRDIMSIYKEPPPGMFVVPDTVD
MTKIHALITGPFDTPYEGGFFLFVFRCPPDYPIHPPRVKLMTTGNNTVRF
NPNFYRNGKVCLSILGTWTGPAWSPAQSISSVLISIQSLMTENPYHNEPG
FEQERHPGDSKNYNECIRHETIRVAVCDMMEGKSPCPEPLRGVMEKSFLE
YYDFYEVACKDRLHLQGQTMQDPFGEKRGHFDYQSLLMRLGLIRQKVLER
LHNENAEMDSDSSSSGTETDLHGSLRV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU29450
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence difference compared with NM_023079.3 CDS: c846t (silent); ; Estimated MW of expressed protein : 40655 Daltons
• Low Temp: No