cDNA Clone UBD (FAT10)

• Plasmid: pTXB1-FAT10 aa1-162 (C7S C9S C134S C160S C162S)-T3C Mxe intein CBD
• Parent Plasmid: pTXB1
• Expressed: FAT10 aa1-162 (C7S C9S C134S C160S C162S)-T3C Mxe intein CBD
• Genbank: O15205
• Tag: Intein
• Species: Human

Sequence of Insert

GCTAGCATGGGTTGCTCTTCCGGCGCTCCCAATGCTTCCTCTCTCTCTGTGCATGTCCGTTCCGAGGAATGGGATTTAATGACCTTTGATGCCAACCCATATGACAGCGTGAAAAAAATCAAAGAACATGTCCGGTCTAAGACCAAGGTTCCTGTGCAGGACCAGGTTCTTTTGCTGGGCTCCAAGATCTTAAAGCCACGGAGAAGCCTCTCATCTTATGGCATTGACAAAGAGAAGACCATCCACCTTACCCTGAAAGTGGTGAAGCCCAGTGATGAGGAGCTGCCCTTGTTTCTTGTGGAGTCAGGTGATGAGGCAAAGAGGCACCTCCTCCAGGTGCGAAGGTCCAGCTCAGTGGCACAAGTGAAAGCAATGATCGAGACTAAGACGGGTATAATCCCTGAGACCCAGATTGTGACTTCCAATGGAAAGAGACTGGAAGATGGGAAGATGATGGCAGATTATGGCATCAGAAAGGGCAACTTACTCTTCCTGGCATCTTATTCTTGCATCTGCGGAGATGCACTAGT

Amino Acid Sequence

MASMGCSSGAPNASSLSVHVRSEEWDLMTFDANPYDSVKKIKEHVRSKTK
VPVQDQVLLLGSKILKPRRSLSSYGIDKEKTIHLTLKVVKPSDEELPLFL
VESGDEAKRHLLQVRRSSSVAQVKAMIETKTGIIPETQIVTSNGKRLEDG
KMMADYGIRKGNLLFLASYSCICGDALVALPEGESVRIADIVPGARPNSD
NAIDLKVLDRHGNPVLADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCL
VDVAGVPTLLWKLIDEIKPGD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU29485
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: additional N-terminal residues (MAS) derived from translation initiation at the pTXB1 vector derived Nde1 site. Native FAT10 Met1 = Met4 of translation product (with GCSSG inserted between native Met1 and Ala2); Intein T3C mutant; ; PARENTAL 45876
• Low Temp: No