Plasmid
pTXB1-ISG15 (aa1-154) C78S T3C Mxe intein CBD
Parent Plasmid
pTXB1
Expressed
ISG15 (aa1-154) C78S T3C Mxe intein CBD
Genbank
NM_005101.4
Tag
Intein
Species
Human
Sequence of Insert
View Sequence
GCTAGCATGGGTTGCTCTTCCGGCGGCTGGGACCTGACGGTGAAGATGCT
GGCGGGCAACGAATTCCAGGTGTCCCTGAGCAGCTCCATGTCGGTGTCAG
AGCTGAAGGCGCAGATCACCCAGAAGATCGGCGTGCACGCCTTCCAGCAG
CGTCTGGCTGTCCACCCGAGCGGTGTGGCGCTGCAGGACAGGGTCCCCCT
TGCCAGCCAGGGCCTGGGCCCCGGCAGCACGGTCCTGCTGGTGGTGGACA
AATCCGACGAACCTCTGAACATCCTGGTGAGGAATAACAAGGGCCGCAGC
AGCACCTACGAGGTGCGGCTGACGCAGACCGTGGCCCACCTGAAGCAGCA
AGTGAGCGGGCTGGAGGGTGTGCAGGACGACCTGTTCTGGCTGACCTTCG
AGGGGAAGCCCCTGGAGGACCAGCTCCCGCTGGGGGAGTACGGCCTCAAG
CCCCTGAGCACCGTGTTCATGAATCTGCGCCTGTGCATCTGCGGAGATGC
ACTAGT
GGCGGGCAACGAATTCCAGGTGTCCCTGAGCAGCTCCATGTCGGTGTCAG
AGCTGAAGGCGCAGATCACCCAGAAGATCGGCGTGCACGCCTTCCAGCAG
CGTCTGGCTGTCCACCCGAGCGGTGTGGCGCTGCAGGACAGGGTCCCCCT
TGCCAGCCAGGGCCTGGGCCCCGGCAGCACGGTCCTGCTGGTGGTGGACA
AATCCGACGAACCTCTGAACATCCTGGTGAGGAATAACAAGGGCCGCAGC
AGCACCTACGAGGTGCGGCTGACGCAGACCGTGGCCCACCTGAAGCAGCA
AGTGAGCGGGCTGGAGGGTGTGCAGGACGACCTGTTCTGGCTGACCTTCG
AGGGGAAGCCCCTGGAGGACCAGCTCCCGCTGGGGGAGTACGGCCTCAAG
CCCCTGAGCACCGTGTTCATGAATCTGCGCCTGTGCATCTGCGGAGATGC
ACTAGT
Amino Acid Sequence
View Sequence
MGCSSGGWDLTVKMLAGNEFQVSLSSSMSVSELKAQITQKIGVHAFQQRL
AVHPSGVALQDRVPLASQGLGPGSTVLLVVDKSDEPLNILVRNNKGRSST
YEVRLTQTVAHLKQQVSGLEGVQDDLFWLTFEGKPLEDQLPLGEYGLKPL
STVFMNLRLCICGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRH
GNPVLADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLW
KLIDEIKPGDYAVIQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLE
AHHRDPDAQAIADELTDGRFYYAKVASVTDAGVQPVYSLRVDTADHAFIT
NGFVSHATGLTGLNSGLTTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQP
HTSLAGWEPSNVPALWQLQ*
AVHPSGVALQDRVPLASQGLGPGSTVLLVVDKSDEPLNILVRNNKGRSST
YEVRLTQTVAHLKQQVSGLEGVQDDLFWLTFEGKPLEDQLPLGEYGLKPL
STVFMNLRLCICGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRH
GNPVLADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLW
KLIDEIKPGDYAVIQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLE
AHHRDPDAQAIADELTDGRFYYAKVASVTDAGVQPVYSLRVDTADHAFIT
NGFVSHATGLTGLNSGLTTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQP
HTSLAGWEPSNVPALWQLQ*
Vector Type
Bacterial
Antibiotic
Amp
Full Snapgene Map
pTXB1-ISG15 (aa1-154) C78S T3C Mxe intein CBD90.72 KBSnapgene files can be opened using software available here: www.snapgene.com
DU Number
DU29486
Unit Source
Location
PPU
MTA
Notes
additional N-terminal residues (MAS) derived from translation initiation at the pTXB1 vector derived Nde1 site. Native ISG15 Met1 = Met4 of translation product (with GCSSG inserted between native Met1 and Gly2)
Intein T3C mutant
Carries known SNP S83N and silent change at Val98 (GTA>GTG) compared to NCBI ref NM_005101.4
PARENTAL 45876
Intein T3C mutant
Carries known SNP S83N and silent change at Val98 (GTA>GTG) compared to NCBI ref NM_005101.4
PARENTAL 45876
Low Temp
No
Price
£125.00
To submit an order for this reagent acceptance of the Dundee Material Transfer Agreement is required at checkout.