cDNA Clone UFM1

• Synonyms: x
• Plasmid: pTXB1-UFM1 (Met1-GCSSG-Ser2-Asp80) T3C Mxe intein CBD
• Parent Plasmid: pTXB1
• Expressed: UFM1-(Met1-GCSSG-Ser2-Asp80) T3C Mxe intein CBD
• Genbank: NM_016617.4 → NP_057701.1
• Tag: Intein
• Species: Human

Sequence of Insert

GCTAGCATGGGTTGCTCTTCCGGCTCGAAGGTTTCCTTTAAGATCACGCT
GACGTCGGACCCACGGCTGCCGTACAAAGTACTCAGTGTTCCTGAAAGTA
CACCTTTCACAGCAGTCTTAAAGTTTGCAGCAGAAGAATTTAAAGTTCCT
GCTGCAACAAGTGCAATTATTACCAATGATGGAATAGGAATAAATCCTGC
ACAGACTGCTGGAAATGTTTTTCTAAAACATGGTTCAGAACTGCGGATTA
TTCCTAGAGATTGCATCTGCGGAGATGCACTAGT

Amino Acid Sequence

MASMGCSSGSKVSFKITLTSDPRLPYKVLSVPESTPFTAVLKFAAEEFKV
PAATSAIITNDGIGINPAQTAGNVFLKHGSELRIIPRDCICGDALVALPE
GESVRIADIVPGARPNSDNAIDLKVLDRHGNPVLADRLFHSGEHPVYTVR
TVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAVIQRSAFSV
DCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFY
YAKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHATGLTGLNSGLTTNP
GVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNVPALWQLQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU29487
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: additional N-terminal residues (MAS) derived from translation initiation at the pTXB1 vector derived Nde1 site. Native UFM1 Met1 = Met4 of translation product (with GCSSG inserted between native Met1 and Ser2); Intein T3C mutant; ; PARENTAL 45876
• Low Temp: No