cDNA Clone HOIL1

• Plasmid: pET15b 6HIS C3-HOIL1 C460S
• Parent Plasmid: pET15b 6His C3
• Expressed: His-HOIL1 [Cys460Ser]
• Genbank: Q9BYM8
• Tag: 6His
• Species: Human

Sequence of Insert

GCGGCCGCGATGGACGAGAAGACCAAGAAAGCAGAGGAAATGGCCCTGAG
CCTCACCCGAGCAGTGGCGGGCGGGGATGAACAGGTGGCAATGAAGTGTG
CCATCTGGCTGGCAGAGCAACGGGTGCCCCTGAGTGTGCAACTGAAGCCT
GAGGTCTCCCCAACGCAGGACATCAGGCTGTGGGTGAGCGTGGAGGATGC
TCAGATGCACACCGTCACCATCTGGCTCACAGTGCGCCCTGATATGACAG
TGGCGTCTCTCAAGGACATGGTTTTTCTGGACTATGGCTTCCCACCAGTC
TTGCAGCAGTGGGTGATTGGGCAGCGGCTGGCACGAGACCAGGAGACCCT
GCACTCCCATGGGGTGCGGCAGAATGGGGACAGTGCCTACCTCTATCTGC
TGTCAGCCCGCAACACCTCCCTCAACCCTCAGGAGCTGCAGCGGGAGCGG
CAGCTGCGGATGCTGGAAGATCTGGGCTTCAAGGACCTCACGCTGCAGCC
GCGGGGCCCTCTGGAGCCAGGCCCCCCAAAGCCCGGGGTCCCCCAGGAAC
CCGGACGGGGGCAGCCAGATGCAGTGCCTGAGCCCCCACCGGTGGGCTGG
CAGTGCCCCGGGTGCACCTTCATCAACAAGCCCACGCGGCCTGGCTGTGA
GATGTGCTGCCGGGCGCGCCCCGAGGCCTACCAGGTCCCCGCCTCATACC
AGCCCGACGAGGAGGAGCGAGCGCGCCTGGCGGGCGAGGAGGAGGCGCTG
CGTCAGTACCAGCAGCGGAAGCAGCAGCAGCAGGAGGGGAACTACCTGCA
GCACGTCCAGCTGGACCAGAGGAGCCTGGTGCTGAACACGGAGCCCGCCG
AGTGCCCCGTGTGCTACTCGGTGCTGGCGCCCGGCGAGGCCGTGGTGCTG
CGTGAGTGTCTGCACACCTTCTGCAGGGAGTGCCTGCAGGGCACCATCCG
CAACAGCCAGGAGGCGGAGGTCTCCTGCCCCTTCATTGACAACACCTACT
CGTGCTCGGGCAAGCTGCTGGAGAGGGAGATCAAGGCGCTCCTGACCCCT
GAGGATTACCAGCGATTTCTAGACCTGGGCATCTCCATTGCTGAAAACCG
CAGTGCCTTCAGCTACCATTGCAAGACCCCAGATTGCAAGGGATGGTGCT
TCTTTGAGGATGATGTCAATGAGTTCACCTGCCCTGTGTGTTTCCACGTC
AACTGCCTGCTCTGCAAGGCCATCCATGAGCAGATGAACTGCAAGGAGTA
TCAGGAGGACCTGGCCCTGCGGGCTCAGAACGATGTGGCTGCCCGGCAGA
CGACAGAGATGCTGAAGGTGATGCTGCAGCAGGGCGAGGCCATGCGCTGC
CCCCAGTGCCAGATCGTGGTACAGAAGAAGGACGGCTcCGACTGGATCCG
CTGCACCGTCTGCCACACCGAGATCTGCTGGGTCACCAAGGGCCCACGCT
GGGGCCCTGGGGGCCCAGGAGACACCAGCGGGGGCTGCCGCTGCAGGGTA
AATGGGATTCCTTGCCACCCAAGCTGTCAGAACTGCCACTGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSPEFPGVDSKAAAMDEKTKKAEEMALSL
TRAVAGGDEQVAMKCAIWLAEQRVPLSVQLKPEVSPTQDIRLWVSVEDAQ
MHTVTIWLTVRPDMTVASLKDMVFLDYGFPPVLQQWVIGQRLARDQETLH
SHGVRQNGDSAYLYLLSARNTSLNPQELQRERQLRMLEDLGFKDLTLQPR
GPLEPGPPKPGVPQEPGRGQPDAVPEPPPVGWQCPGCTFINKPTRPGCEM
CCRARPEAYQVPASYQPDEEERARLAGEEEALRQYQQRKQQQQEGNYLQH
VQLDQRSLVLNTEPAECPVCYSVLAPGEAVVLRECLHTFCRECLQGTIRN
SQEAEVSCPFIDNTYSCSGKLLEREIKALLTPEDYQRFLDLGISIAENRS
AFSYHCKTPDCKGWCFFEDDVNEFTCPVCFHVNCLLCKAIHEQMNCKEYQ
EDLALRAQNDVAARQTTEMLKVMLQQGEAMRCPQCQIVVQKKDGSDWIRC
TVCHTEICWVTKGPRWGPGGPGDTSGGCRCRVNGIPCHPSCQNCH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU29604
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No