cDNA Clone IRAK4

• Plasmid: pCMV5 FLAG1 IRAK4 K440E
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG IRAK4 K440E
• Genbank: NM_016123.3
• Tag: FLAG
• Species: Human

Sequence of Insert

ggatccatgaacaaacccataacaccatcaacatatgtgcgctgcctcaa
tgttggactaattaggaagctgtcagattttattgatcctcaagaaggat
ggaagaagttagctgtagctattaaaaaaccatctggtgatgatagatac
aatcagtttcacataaggagatttgaagcattacttcaaactggaaaaag
tcccacttctgaattactgtttgactggggcaccacaaattgcacagttg
gtgatcttgtggatcttttgatccaaaatgaattttttgctcctgcaagt
cttttgctcccagatgctgttcccaaaactgctaatacactaccttctaa
agaagctataacagttcagcaaaaacagatgcctttctgtgacaaagaca
ggacattgatgacacctgtgcagaatcttgaacaaagctatatgccacct
gactcctcaagtccagaaaataaaagtttagaagttagtgatacacgttt
tcacagtttttcattttatgaattgaagaatgtcacaaataactttgatg
aacgacccatttctgttggtggtaataaaatgggagagggaggatttgga
gttgtatataaaggctacgtaaataacacaactgtggcagtgaagaagct
tgcagcaatggttgacattactactgaagaactgaaacagcagtttgatc
aagaaataaaagtaatggcaaagtgtcaacatgaaaacttagtagaacta
cttggtttctcaagtgatggagatgacctctgcttagtatatgtttacat
gcctaatggttcattgctagacagactctcttgcttggatggtactccac
cactttcttggcacatgagatgcaagattgctcagggtgcagctaatggc
atcaattttctacatgaaaatcatcatattcatagagatattaaaagtgc
aaatatcttactggatgaagcttttactgctaaaatatctgactttggcc
ttgcacgggcttctgagaagtttgcccagacagtcatgactagcagaatt
gtgggaacaacagcttatatggcaccagaagctttgcgtggagaaataac
acccaaatctgatatttacagctttggtgtggttttactagaaataataa
ctggacttccagctgtggatgaacaccgtgaacctcagttattgctagat
attaaagaagaaattgaagatgaagaaaagacaattgaagattatattga
taaaaagatgaatgatgctgattccacttcagttgaagctatgtactctg
ttgctagtcaatgtctgcatgaaGagaaaaataagagaccagacattaag
aaggttcaacagctgctgcaagagatgacagcttcttaagcggccgc

Amino Acid Sequence

MDYKDDDDKGSMNKPITPSTYVRCLNVGLIRKLSDFIDPQEGWKKLAVAI
KKPSGDDRYNQFHIRRFEALLQTGKSPTSELLFDWGTTNCTVGDLVDLLI
QNEFFAPASLLLPDAVPKTANTLPSKEAITVQQKQMPFCDKDRTLMTPVQ
NLEQSYMPPDSSSPENKSLEVSDTRFHSFSFYELKNVTNNFDERPISVGG
NKMGEGGFGVVYKGYVNNTTVAVKKLAAMVDITTEELKQQFDQEIKVMAK
CQHENLVELLGFSSDGDDLCLVYVYMPNGSLLDRLSCLDGTPPLSWHMRC
KIAQGAANGINFLHENHHIHRDIKSANILLDEAFTAKISDFGLARASEKF
AQTVMTSRIVGTTAYMAPEALRGEITPKSDIYSFGVVLLEIITGLPAVDE
HREPQLLLDIKEEIEDEEKTIEDYIDKKMNDADSTSVEAMYSVASQCLHE
EKNKRPDIKKVQQLLQEMTAS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU29713
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: silent change g291a compared to NCBI ref
• Low Temp: No