cDNA Clone HERC5

• Synonyms: HERC5 HECT and RLD domain containing E3 ubiquitin protein ligase 5
• Plasmid: pSc-B-HERC5 E692-G1024 (end)
• Parent Plasmid: pSC-B
• Genbank: NM_016323.3 → NP_057407.2 Q9UII4 Gene 51191
• Species: Human

Sequence of Insert

ggatccGAGGATGTTTTGAATCAGCTAAGTCAATTTGAGAATGAAGACCT
GAGGAAAGAGTTATGGGTTTCATTTAGTGGAGAAATTGGGTATGACCTCG
GAGGAGTCAAGAAAGAGTTCTTCTACTGTCTGTTTGCAGAGATGATCCAG
CCGGAATATGGGATGTTCATGTATCCTGAAGGGGCTTCCTGCATGTGGTT
TCCTGTCAAGCCTAAATTTGAGAAGAAAAGATACTTCTTTTTTGGGGTTC
TATGTGGACTTTCCCTGTTCAATTGCAATGTTGCCAACCTCCCTTTCCCA
CTGGCACTGTTTAAGAAACTTTTGGACCAAATGCCATCATTGGAAGACTT
GAAAGAACTCAGTCCTGATTTGGGAAAGAATTTGCAAACACTTCTGGATG
ATGAAGGTGATAACTTTGAGGAAGTATTTTACATCCATTTTAATGTGCAC
TGGGACAGAAACGACACAAACTTAATTCCTAATGGAAGTAGCATAACTGT
CAACCAGACTAACAAGAGAGACTATGTTTCTAAGTATATCAATTACATTT
TCAACGACTCTGTAAAGGCGGTTTATGAAGAATTTCGGAGAGGATTTTAT
AAAATGTGCGACGAAGACATTATCAAATTATTCCACCCCGAAGAACTGAA
GGATGTGATTGTTGGAAATACAGATTATGATTGGAAAACATTTGAAAAGA
ATGCACGTTATGAACCAGGATATAACAGTTCACATCCCACCATAGTGATG
TTTTGGAAGGCTTTCCACAAATTGACTCTGGAAGAAAAGAAAAAATTCCT
TGTATTTCTTACAGGAACTGACAGACTACAAATGAAAGATTTAAATAATA
TGAAAATAACATTTTGCTGTCCTGAAAGTTGGAATGAAAGAGACCCTATA
AGAGCACTGACATGTTTCAGTGTCCTCTTCCTCCCTAAATATTCTACAAT
GGAAACAGTTGAAGAAGCGCTTCAAGAAGCCATCAACAACAACAGAGGAT
TTGGCTGAgcggccgc

Amino Acid Sequence

EDVLNQLSQFENEDLRKELWVSFSGEIGYDLGGVKKEFFYCLFAEMIQPE
YGMFMYPEGASCMWFPVKPKFEKKRYFFFGVLCGLSLFNCNVANLPFPLA
LFKKLLDQMPSLEDLKELSPDLGKNLQTLLDDEGDNFEEVFYIHFNVHWD
RNDTNLIPNGSSITVNQTNKRDYVSKYINYIFNDSVKAVYEEFRRGFYKM
CDEDIIKLFHPEELKDVIVGNTDYDWKTFEKNARYEPGYNSSHPTIVMFW
KAFHKLTLEEKKKFLVFLTGTDRLQMKDLNNMKITFCCPESWNERDPIRA
LTCFSVLFLPKYSTMETVEEALQEAINNNRGFG*

• Vector Type: Other
• Antibiotic: Amp; Kan
• DU Number: DU29696
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent change at Leu786 (CTT > CTC); plus stop codon
• Low Temp: No