cDNA Clone Ufc1(mouse)

• Plasmid: pGEX6P1 Ufc1(mouse)(K0)
• Parent Plasmid: pGEX6P1
• Expressed: GST-C3-Ufc1(mouse)(K0)
• Genbank: Q9CR09 NM_025388.2
• Tag: GST

Sequence of Insert

GGATCCATGGCGGACGAGGCCACTCGGCGGGTCGTGTCTGAGATCCCGGTGCTGAGGACCAACGCCGGACCCCGAGATCGGGAATTGTGGGTGCAGCGACTAAGGGAGGAATATCAGTCCCTGATCCGGTATGTGGAGAACAACAGGAATTCGGACAATGATTGGTTCCGACTGGAGTCCAACAGGGAAGGGACCCGGTGGTTTGGAAGATGCTGGTACATCCACGATTTCCTCAGATATGAGTTCGACATCGAGTTTGAAATTCCTATCACATATCCCACTACTGCTCCGGAAATTGCAGTCCCTGAGCTGGACGGGAGGACAGCAAGGATGTACAGGGGTGGCAGAATATGTCTGACTGATCATTTCAGACCTTTGTGGGCCAGGAATGTGCCTAGGTTTGGACTAGCTCATCTCATGGCCCTGGGGCTGGGTCCTTGGCTGGCAGTGGAAGTCCCCGATCTGATCCAGAGGGGTGTGATCCAGCACAGAGAAAGATGCAGCCAATGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMADEATRRVVSEIPVLRTNAGPRDRELWVQRLREEYQSLIRYVENNRNSDNDWFRLESNREGTRWFGRCWYIHDFLRYEFDIEFEIPITYPTTAPEIAVPELDGRTARMYRGGRICLTDHFRPLWARNVPRFGLAHLMALGLGPWLAVEVPDLIQRGVIQHRERCSQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU59746
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Kindly mutate all lysine in Ufc1 to Arginine. ; (K17R, K33R , K47R ,K60R ,K68R ,K77R ,K105R ,K108R ,K114R ,K122R ,K131R ,K156R ,K162R ,K164R)
• Low Temp: No