cDNA Clone UbcH5c

• Synonyms: UBE2D3
• Plasmid: pET15b 6His C3 UBCH5c S22R F62A C85K
• Parent Plasmid: pET15b 6His C3
• Expressed: His-UbcH5c S22R F62A C85K
• Genbank: NM_003340.6 → NP_003331.1 Gene ID 7323
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCGCGCTGAAACGGATTAATAAGGAACTTAGTGATTTGGCCCGTGA
CCCTCCAGCACAATGTCGTGCAGGTCCAGTTGGGGATGATATGTTTCATT
GGCAAGCCACAATTATGGGACCTAATGACAGCCCATATCAAGGCGGTGTA
TTCTTTTTGACAATTCATTTTCCTACAGACTACCCCgcCAAACCACCTAA
GGTTGCATTTACAACAAGAATTTATCATCCAAATATTAACAGTAATGGCA
GCATTAAACTCGATATTCTAAGATCACAGTGGTCGCCTGCTTTAACAATT
TCTAAAGTTCTTTTATCCATTTGTTCACTGCTATGTGATCCAAACCCAGA
TGACCCCCTAGTGCCAGAGATTGCACGGATCTATAAAACAGACAGAGATA
AGTACAACAGAATATCTCGGGAATGGACTCAGAAGTATGCCATGTGAGCG
GCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSALKRINKELSDLARDPPAQCRAGPVGD
DMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPAKPPKVAFTTRIYHPNI
NSNGSIKLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYK
TDRDKYNRISREWTQKYAM*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU29756
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: isoform 1; UbcH5c ORF has no native initiating methionine
• Low Temp: No