cDNA Clone HOIL1

• Plasmid: pET156P HOIL1 T203A/R210A
• Parent Plasmid: pET156P
• Expressed: His-HOIL1 T203A/R210A
• Genbank: Q9BYM8 NP_112506.2
• Tag: 6His
• Species: Human

Sequence of Insert

GCGGCCGCGATGGACGAGAAGACCAAGAAAGCAGAGGAAATGGCCCTGAG
CCTCACCCGAGCAGTGGCGGGCGGGGATGAACAGGTGGCAATGAAGTGTG
CCATCTGGCTGGCAGAGCAACGGGTGCCCCTGAGTGTGCAACTGAAGCCT
GAGGTCTCCCCAACGCAGGACATCAGGCTGTGGGTGAGCGTGGAGGATGC
TCAGATGCACACCGTCACCATCTGGCTCACAGTGCGCCCTGATATGACAG
TGGCGTCTCTCAAGGACATGGTTTTTCTGGACTATGGCTTCCCACCAGTC
TTGCAGCAGTGGGTGATTGGGCAGCGGCTGGCACGAGACCAGGAGACCCT
GCACTCCCATGGGGTGCGGCAGAATGGGGACAGTGCCTACCTCTATCTGC
TGTCAGCCCGCAACACCTCCCTCAACCCTCAGGAGCTGCAGCGGGAGCGG
CAGCTGCGGATGCTGGAAGATCTGGGCTTCAAGGACCTCACGCTGCAGCC
GCGGGGCCCTCTGGAGCCAGGCCCCCCAAAGCCCGGGGTCCCCCAGGAAC
CCGGACGGGGGCAGCCAGATGCAGTGCCTGAGCCCCCACCGGTGGGCTGG
CAGTGCCCCGGGTGCgCCTTCATCAACAAGCCCACGgcGCCTGGCTGTGA
GATGTGCTGCCGGGCGCGCCCCGAGGCCTACCAGGTCCCCGCCTCATACC
AGCCCGACGAGGAGGAGCGAGCGCGCCTGGCGGGCGAGGAGGAGGCGCTG
CGTCAGTACCAGCAGCGGAAGCAGCAGCAGCAGGAGGGGAACTACCTGCA
GCACGTCCAGCTGGACCAGAGGAGCCTGGTGCTGAACACGGAGCCCGCCG
AGTGCCCCGTGTGCTACTCGGTGCTGGCGCCCGGCGAGGCCGTGGTGCTG
CGTGAGTGTCTGCACACCTTCTGCAGGGAGTGCCTGCAGGGCACCATCCG
CAACAGCCAGGAGGCGGAGGTCTCCTGCCCCTTCATTGACAACACCTACT
CGTGCTCGGGCAAGCTGCTGGAGAGGGAGATCAAGGCGCTCCTGACCCCT
GAGGATTACCAGCGATTTCTAGACCTGGGCATCTCCATTGCTGAAAACCG
CAGTGCCTTCAGCTACCATTGCAAGACCCCAGATTGCAAGGGATGGTGCT
TCTTTGAGGATGATGTCAATGAGTTCACCTGCCCTGTGTGTTTCCACGTC
AACTGCCTGCTCTGCAAGGCCATCCATGAGCAGATGAACTGCAAGGAGTA
TCAGGAGGACCTGGCCCTGCGGGCTCAGAACGATGTGGCTGCCCGGCAGA
CGACAGAGATGCTGAAGGTGATGCTGCAGCAGGGCGAGGCCATGCGCTGC
CCCCAGTGCCAGATCGTGGTACAGAAGAAGGACGGCTGCGACTGGATCCG
CTGCACCGTCTGCCACACCGAGATCTGCTGGGTCACCAAGGGCCCACGCT
GGGGCCCTGGGGGCCCAGGAGACACCAGCGGGGGCTGCCGCTGCAGGGTA
AATGGGATTCCTTGCCACCCAAGCTGTCAGAACTGCCACTGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSPEFPGVDSKAAAMDEKTKKAEEMALSL
TRAVAGGDEQVAMKCAIWLAEQRVPLSVQLKPEVSPTQDIRLWVSVEDAQ
MHTVTIWLTVRPDMTVASLKDMVFLDYGFPPVLQQWVIGQRLARDQETLH
SHGVRQNGDSAYLYLLSARNTSLNPQELQRERQLRMLEDLGFKDLTLQPR
GPLEPGPPKPGVPQEPGRGQPDAVPEPPPVGWQCPGCAFINKPTAPGCEM
CCRARPEAYQVPASYQPDEEERARLAGEEEALRQYQQRKQQQQEGNYLQH
VQLDQRSLVLNTEPAECPVCYSVLAPGEAVVLRECLHTFCRECLQGTIRN
SQEAEVSCPFIDNTYSCSGKLLEREIKALLTPEDYQRFLDLGISIAENRS
AFSYHCKTPDCKGWCFFEDDVNEFTCPVCFHVNCLLCKAIHEQMNCKEYQ
EDLALRAQNDVAARQTTEMLKVMLQQGEAMRCPQCQIVVQKKDGCDWIRC
TVCHTEICWVTKGPRWGPGGPGDTSGGCRCRVNGIPCHPSCQNCH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU29853
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No