cDNA Clone ubiquitin

• Plasmid: pET15-M1-GCSSG-Q2-UBIQUITIN-6HIS TAG11
• Parent Plasmid: pET156P-1
• Expressed: M1-GCSSG-Q2-ubiquitin-His TAG11
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGGTTGCTCTTCCGGCCAGATCTTCGTGAAGACCCTGACTGGTTAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCCGAGGTGGGCATCACCATCACCATCACTAAGCGGCCGC

Amino Acid Sequence

MGCSSGQIFVKTLTG*TITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
AGKQLEDGRTLSDYNIQKESTLHLVLRLRGGHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU29924
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: GCSSG inserted between M1 and Q2 of ubiquitin ORF.; C-terminal Hisx6 tag immediately follows GG (retain TAA stop codon).
• Low Temp: No