cDNA Clone Ubiquitin

• Plasmid: pET24-Ubiquitin Thr12Glu
• Parent Plasmid: pET24a
• Expressed: Ubiquitin [Thr12SGlu]
• Genbank: P62988
• Species: Human

Sequence of Insert

CATATGCAGATCTTCGTGAAGACCCTGACTGGTAAGGAGATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCCGAGGTGGGTGATAAGGATCC

Amino Acid Sequence

MQIFVKTLTGKEITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQL
EDGRTLSDYNIQKESTLHLVLRLRGG**

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU29929
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct for untagged ubiquitin (Arg73 codon AGA mutated to more common CGA) Differs from reference sequence in having silent mutation at Arg73 (a220c substitution conferred by reverse primer 54389). T7 tag in pET24a removed by subcloning into the Nde1 site, to give untagged protein.
• Low Temp: No