cDNA Clone UbcH5C

• Plasmid: p6HisMEX-UBCH5c C21S 107S C111S
• Expressed: His-MBP-UbcH5C C21S 107S C111S
• Genbank: NM_003340.6 → NP_003331.1 Gene ID 7323
• Tag: 6His
• Tag: MBP
• Species: Human

Sequence of Insert

GGATCCGCGCTGAAACGGATTAATAAGGAACTTAGTGATTTGGCCCGTGA
CCCTCCAGCACAATCTTCTGCAGGTCCAGTTGGGGATGATATGTTTCATT
GGCAAGCCACAATTATGGGACCTAATGACAGCCCATATCAAGGCGGTGTA
TTCTTTTTGACAATTCATTTTCCTACAGACTACCCCTTCAAACCACCTAA
GGTTGCATTTACAACAAGAATTTATCATCCAAATATTAACAGTAATGGCA
GCATTTGTCTCGATATTCTAAGATCACAGTGGTCGCCTGCTTTAACAATT
TCTAAAGTTCTTTTATCCATTTCTTCACTGCTATCTGATCCAAACCCAGA
TGACCCCCTAGTGCCAGAGATTGCACGGATCTATAAAACAGACAGAGATA
AGTACAACAGAATATCTCGGGAATGGACTCAGAAGTATGCCATGTGAGCG
GCCGC

Amino Acid Sequence

MHHHHHHMKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDK
LEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPF
TWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAK
GKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNY
GVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEA
VNKDKPLGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYA
VRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFL
EVLFQGPLGSALKRINKELSDLARDPPAQSSAGPVGDDMFHWQATIMGPN
DSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRS
QWSPALTISKVLLSISSLLSDPNPDDPLVPEIARIYKTDRDKYNRISREW
TQKYAM*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU29995
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: sub cloned into Bam-Not backbone of 26341
• Low Temp: No