cDNA Clone HUWE1

• Plasmid: pcDNA FRT TP GFP HUWE1
• Parent Plasmid: HUWE1
• Expressed: GFP-HUWE1
• Genbank: NM-031407.6
• Tag: GFP

Sequence of Insert

GGATCTATGAAAGTAGACAGGACTAAACTGAAGAAGACACCTACTGAGGCTCCTGCAGACTGCAGAGCCTTAATAGACAAACTCAAAGTTTGTAATGATGAGCAACTTCTCTTGGAACTGCAGCAGATCAAAACATGGAACATTGGAAAGTGCGAGTTATATCACTGGGTGGACCTGTTGGACCGCTTCGATGGAATACTGGCAGATGCTGGACAGACAGTGGAGAATATGTCATGGATGCTCGTATGTGATAGGCCAGAAAGAGAGCAACTGAAAATGCTTCTCTTGGCTGTGTTGAACTTCACAGCCTTGCTCATTGAGTACAGCTTTTCCCGGCATCTGTACAGTTCCATAGAGCATTTGACAACTTTATTGGCTTCCTCTGATATGCAAGTGGTGCTGGCAGTCCTCAATCTCCTATATGTATTTAGCAAAAGATCAAACTACATCACTCGTCTGGGATCTGACAAGAGGACCCCGCTGCTAACTCGGCTACAACATTTGGCAGAGAGCTGGGGTGGAAAGGAGAATGGCTTTGGACTTGCAGAATGTTGCAGAGACTTGCATATGATGAAATATCCACCCAGTGCAACTACACTACACTTTGAATTCTACGCAGATCCTGGGGCCGAGGTCAAAATTGAGAAAAGGACAACTAGTAACACACTACATTATATTCACATAGAGCAACTTGACAAGATTTCAGAAAGCCCTTCTGAAATCATGGAATCTCTTACCAAAATGTACAGCATTCCTAAGGATAAGCAGATGCTGTTATTTACACACATACGACTGGCCCATGGCTTTTCTAATCACAGGAAGCGATTGCAGGCAGTTCAGGCCAGACTGCATGCAATATCTATATTAGTGTATTCCAATGCCTTGCAGGAATCAGCAAACAGTATCTTGTATAATGGCTTGATAGAGGAGTTGGTAGATGTCCTTCAGATAACGGATAAGCAGCTTATGGAGATTAAAGCAGCTTCTTTACGAACATTAACATCAATTGTCCACTTGGAGAGAACTCCCAAACTCAGCAGTATTATTGACTGTACTGGAACTGCCTCCTACCATGGATTTTTGCCAGTGCTTGTAAGGAACTGTATCCAGGCCATGATTGATCCTTCCATGGATCCATACCCTCACCAGTTTGCCACTGCTCTCTTCTCTTTTTTATACCATCTGGCCAGCTACGATGCTGGTGGTGAAGCCTTGGTCTCCTGTGGAATGATGGAAGCCTTATTGAAGGTCATAAAGTTTCTTGGCGATGAACAGGACCAGATAACATTTGTCACCAGAGCCGTCAGAGTGGTTGACCTTATCACCAACCTGGATATGGCAGCTTTTCAATCCCATAGTGGACTTTCTATCTTCATTTATAGACTTGAGCATGAAGTAGATTTGTGCCGAAAAGAATGTCCGTTTGTGATCAAGCCAAAGATCCAGAGACCCAATACTACACAAGAAGGAGAGGAAATGGAAACTGATATGGATGGAGTCCAGTGTATTCCACAACGAGCAGTACTTCTGAAATCCATGTTGAATTTCCTCAAGAAGGCCATCCAAGACCCTACTTTCTCAGATGGCATACGACATGTGATGGATGGTTCTCTGCCTACCTCCCTGAAACACATCATCAGCAATGCAGAATACTATGGCCCATCACTCTTCCTCCTAGCTACTGAAGTGGTGACTGTGTTTGTATTTCAAGAACCATCACTGCTCTCCTCACTCCAGGACAATGGATTGACAGATGTCATGCTGCATGCACTGCTTATCAAAGATGTTCCTGCTACCCGTGAAGTCCTTGGCTCCCTCCCAAATGTATTCAGTGCACTCTGTTTGAATGCCCGAGGTCTTCAGTCTTTTGTTCAGTGTCAGCCTTTTGAACGCCTCTTCAAAGTTCTTCTGTCTCCAGATTACCTCCCAGCCATGCGGAGGAGGAGAAGTTCTGATCCCCTTGGGGATACTGCATCCAACCTGGGGAGTGCTGTCGATGAGCTCATGAGACATCAGCCCACCCTTAAAACAGATGCAACGACTGCCATCATCAAGTTACTTGAAGAAATCTGTAATCTTGGAAGGGACCCCAAATACATCTGTCAGAAGCCATCAATCCAGAAGGCAGATGGCACTGCCACTGCTCCTCCCCCAAGGTCTAATCATGCCGCAGAAGAAGCCTCTAGTGAGGATGAGGAGGAAGAGGAAGTACAGGCCATGCAGAGCTTTAATTCTACCCAGCAAAATGAAACTGAGCCTAATCAGCAGGTTGTTGGTACAGAGGAACGTATTCCTATTCCCCTCATGGATTACATCCTTAATGTGATGAAATTTGCGGAATCTATTCTGAGCAACAATACAACAGATGACCACTGCCAGGAATTTGTGAATCAGAAAGGACTGTTGCCTTTGGTTACCATTTTGGGTCTTCCCAATCTGCCCATTGACTTTCCCACATCTGCTGCCTGTCAGGCTGTTGCAGGTGTCTGCAAATCCATATTGACACTGTCACATGAACCCAAAGTCCTTCAAGAGGGTCTCCTTCAGTTGGACTCCATCCTCTCCTCCCTGGAGCCCTTACACCGCCCCATTGAATCCCCTGGGGGCTCAGTGTTGTTGCGAGAACTGGCTTGCGCAGGCAATGTTGCTGGTGCTACCCTCTCAGCCCAGGCCACACCTCTGCTGCATGCACTCACTGCTGCCCATGCCTACATCATGATGTTTGTTCATACTTGCAGAGTTGGACAGAGTGAAATTCGTTCCATCTCCGTAAACCAGTGGGGCTCTCAATTGGGTCTGAGTGTTTTGAGCAAGCTGAGCCAGTTATACTGTTCCCTGGTGTGGGAAAGCACTGTCCTCCTCTCTCTGTGTACCCCAAACAGCCTACCATCTGGGTGTGAATTTGGCCAGGCAGATATGCAGAAACTGGTTCCAAAGGATGAGAAGGCAGGTACGACCCAGGGTGGAAAAAGATCAGATGGGGAACAGGATGGAGCAGCTGGAAGTATGGATGCTTCTACCCAGGGCTTATTAGAAGGCATTGGGCTAGATGGTGACACATTGGCTCCCATGGAGACAGATGAACCTACTGCTTCAGACTCTAAGGGCAAATCTAAAATCACACCAGCAATGGCTGCCAGAATTAAGCAAATCAAGCCTTTGTTATCAGCTTCCTCCAGATTAGGCCGAGCACTTGCTGAGCTATTTGGACTTCTTGTTAAACTTTGTGTGGGATCTCCTGTCCGCCAGAGAAGGAGCCATCATGCTGCCAGCACCACTACAGCACCGACACCTGCCGCGCGATCAACAGCCTCAGCTCTCACTAAGCTCTTGACTAAGGGGTTATCTTGGCAGCCCCCACCATATACACCTACTCCCCGATTCAGGCTGACATTCTTCATCTGTTCAGTTGGTTTCACATCCCCAATGCTGTTTGATGAGAGGAAGTATCCCTACCACCTCATGCTGCAAAAATTTCTCTGCTCCGGAGGCCACAATGCTCTTTTTGAAACTTTCAACTGGGCTCTGTCCATGGGAGGTAAAGTTCCTGTTTCTGAGGGATTGGAACACTCAGACTTGCCTGATGGCACAGGAGAATTCCTAGATGCCTGGCTTATGCTGGTGGAGAAGATGGTGAATCCCACCACGGTGCTTGAATCTCCACATTCGCTGCCTGCCAAATTGCCTGGAGGTGTCCAGAACTTTCCCCAGTTCAGTGCACTGCGCTTCCTTGTGGTAACTCAGAAAGCAGCCTTTACTTGCATCAAAAACTTATGGAACCGGAAACCCCTGAAGGTATATGGTGGACGAATGGCTGAATCGATGCTGGCCATTCTATGCCACATCCTCCGAGGAGAACCTGTGATTCGAGAGAGACTAAGCAAGGAGAAGGAGGGGTCTCGAGGAGAAGAGGATACAGGGCAAGAGGAAGGTGGCTCCCGCCGGGAACCTCAAGTCAACCAGCAACAACTACAACAGCTCATGGACATGGGCTTCACAAGGGAACATGCAATGGAGGCACTGTTGAACACCAGCACCATGGAGCAGGCCACAGAGTACCTTTTAACCCACCCTCCTCCAATCATGGGAGGAGTTGTTCGGGATCTCAGCATGTCTGAAGAGGACCAGATGATGAGAGCAATTGCTATGTCTCTGGGACAGGATATTCCAATGGATCAAAGGGCAGAGTCACCTGAGGAAGTTGCTTGCCGGAAGGAGGAAGAGGAACGGAAAGCTCGGGAAAAGCAGGAGGAGGAAGAGGCTAAATGTCTAGAGAAGTTCCAGGATGCTGACCCGTTGGAACAAGATGAGCTCCACACTTTCACAGATACTATGTTGCCAGGCTGCTTCCACCTTCTTGATGAGCTGCCGGACACAGTATACCGTGTGTGTGACCTGATCATGACAGCAATCAAACGTAATGGAGCAGATTATCGTGACATGATTCTGAAGCAAGTAGTCAATCAGGTGTGGGAAGCTGCTGATGTATTGATCAAAGCTGCTCTTCCCCTGACAACAAGTGACACAAAAACCGTGTCAGAGTGGATAAGTCAGATGGCCACACTGCCCCAGGCCTCCAATTTGGCTACTAGAATCTTGCTTTTAACGCTACTTTTTGAGGAGTTGAAGCTACCTTGTGCTTGGGTGGTTGAATCAAGTGGCATCCTTAATGTCCTAATCAAACTCTTGGAAGTGGTTCAGCCCTGCCTCCAGGCAGCCAAGGAGCAGAAGGAAGTCCAGACCCCAAAGTGGATCACACCAGTGTTGCTCCTGATTGATTTCTATGAAAAGACAGCCATCTCCTCAAAAAGGAGAGCCCAGATGACTAAGTACCTGCAATCCAACAGCAACAACTGGCGCTGGTTTGATGATCGCTCTGGGCGTTGGTGTAGTTACAGTGCAAGCAACAATAGCACTATTGATTCTGCCTGGAAATCTGGAGAGACAAGCGTGCGATTCACTGCAGGCCGAAGAAGATACACGGTCCAATTCACTACAATGGTGCAGGTTAATGAGGAAACAGGGAACCGACGCCCTGTGATGCTGACTCTCCTCAGGGTACCTCGGCTGAATAAAAATTCAAAAAACAGCAATGGACAGGAACTAGAGAAGACGCTGGAAGAAAGCAAAGAAATGGATATCAAACGTAAAGAAAATAAAGGCAATGATACCCCTTTGGCCCTAGAGAGTACAAACACTGAAAAGGAGACAAGCCTGGAGGAAACAAAAATCGGGGAGATCCTGATCCAGGGCTTGACAGAAGATATGGTGACTGTTTTAATCCGGGCCTGCGTGAGCATGCTGGGAGTCCCTGTGGACCCAGATACTTTGCATGCCACCCTTCGTCTCTGTCTGAGGCTCACCCGGGACCACAAATATGCCATGATGTTTGCAGAACTGAAGAGTACCCGCATGATCTTGAATTTGACCCAGAGCTCAGGCTTCAATGGGTTTACTCCCCTGGTCACCCTTCTCTTAAGACACATCGTTGAGGACCCCTGTACCCTTCGTCATACCATGGAAAAGGTCGTTCGCTCAGCAGCTACAAGTGGAGCTGGTAGCACTACCTCTGGTGTTGTGTCTGGCAGCCTCGGCTCTCGGGAGATCAACTACATCCTTCGTGTCCTTGGGCCAGCCGCATGCCGCAATCCAGACATATTCACAGAAGTGGCCAACTGCTGTATCCGCATCGCCCTTCCTGCCCCTCGAGGCTCAGGAACTGCTTCAGATGATGAATTTGAGAATCTTAGAATTAAAGGCCCTAATGCTGTACAGCTGGTGAAGACCACCCCTTTGAAGCCCTCACCTCTGCCTGTCATCCCTGATACTATCAAGGAAGTGATCTATGATATGCTGAATGCTCTGGCTGCATACCATGCTCCAGAGGAAGCAGATAAATCTGATCCTAAACCTGGGGTTATGACCCAAGAGGTTGGCCAGCTCCTGCAAGACATGGGTGATGATGTATACCAGCAGTACCGGTCACTTACGCGTCAGAGCAGTGACTTTGATACGCAGTCAGGTTTTTCCATTAATAGTCAGGTCTTTGCTGCAGATGGTGCCTCCACTGAGACTTCCGCATCTGGGACCTCCCAAGGAGAGGCTTCAACTCCAGAGGAGTCTCGAGATGGGAAGAAAGATAAAGAAGGGGACCGGGCCTCTGAGGAAGGCAAACAGAAAGGCAAGGGCAGCAAACCTTTAATGCCTACCTCCACTATCCTTCGTCTTCTGGCAGAGTTGGTGAGGTCCTATGTTGGTATTGCTACCCTGATTGCCAACTACAGCTACACTGTGGGCCAGTCTGAACTGATCAAAGAGGACTGCAGTGTGCTAGCTTTTGTTCTGGACCACCTGCTCCCACATACCCAGAATGCAGAAGACAAGGACACCCCTGCCTTGGCCCGCCTGTTCCTCGCAAGCCTGGCTGCTGCAGGGAGTGGCACAGATGCCCAGGTGGCCCTAGTGAATGAAGTAAAAGCAGCCCTTGGACGGGCACTGGCTATGGCTGAGAGTACAGAGAAACATGCCAGGCTTCAGGCAGTGATGTGTATCATCAGTACTATCATGGAGTCCTGCCCCTCCACCTCCAGCTTCTACAGCAGTGCCACAGCGAAGACCCAGCACAATGGCATGAACAACATCATTCGGCTTTTCCTGAAGAAGGGACTGGTTAATGACCTGGCCAGAGTACCTCACAGCTTAGACCTGTCCAGTCCCAACATGGCCAACACAGTCAATGCTGCTCTGAAGCCTTTGGAAACACTTTCCCGGATTGTGAACCAGCCCAGTAGCCTTTTTGGCAGCAAGAGTGCTTCTAGCAAGAACAAGTCTGAGCAGGATGCCCAAGGAGCCTCTCAAGATTCCAGTAGCAACCAGCAGGACCCAGGCGAGCCTGGGGAAGCAGAAGTGCAGGAGGAGGATCATGATGTCACTCAGACAGAGGTGGCAGATGGGGATATCATGGATGGGGAGGCTGAAACCGACTCAGTGGTGATTGCTGGGCAGCCTGAGGTGCTCAGTTCACAAGAGATGCAGGTTGAGAATGAGCTGGAGGACCTGATAGATGAGTTGCTTGAGAGGGATGGCGGATCTGGGAACAGTACAATTATAGTGAGCAGAAGTGGAGAGGATGAATCACAAGAGGACGTGCTGATGGATGAAGCTCCTTCCAACCTCAGCCAAGCTTCCACCTTGCAGGCCAACCGAGAAGATTCCATGAATATCCTGGACCCTGAGGATGAGGAGGAGCACACTCAGGAAGAGGACAGCAGTGGCAGTAACGAGGATGAGGATGATAGTCAGCATGAAGAGGAGGAGGAGGAGGAAGATGAGGAAGATGATCAGGAGGATGATGAAGGTGAAGAGGGAGATGAAGACGATGACGACGATGGCTCTGAGATGGAATTGGATGAGGATTATCCTGATATGAACGCTTCTCCCTTGGTCCGATTTGAGCGCTTTGACCGGGAGGATGATCTCATCATTGAGTTTGACAACATGTTCTCCAGTGCTACAGACATCCCCCCATCCCCAGGAAATATCCCTACCACCCATCCACTGATGGTGTGCCATGCAGACCACAGTTCTCTGACACTGGGCAGTGGCTCTTCAACAACTCGTCTCACCCAGGGCATCGGGCGCAGTCAGAGGACCCTAAGGCAGCTGACGGCCAATACTGGCCACACCATTCATGTTCACTACCCTGGGAATCGCCAGCCCAACCCTCCTCTTATACTGCAGAGGTTGCTTGGTCCCTCAGCTGCTGCTGACATCCTTCAGCTGAGCAGCAGCCTTCCCCTACAAAGCCGGGGTCGGGCCCGCCTCCTGGTAGGCAACGATGACGTCCACATCATCGCCCGTTCTGATGATGAGCTGCTGGATGACTTTTTCCATGATCAGAGCACAGCTACCAGCCAAGCAGGAACCCTGTCCAGCATCCCCACAGCCCTGACCCGCTGGACAGAAGAATGCAAAGTTCTCGATGCTGAGAGCATGCATGACTGTGTTTCAGTGGTTAAAGTGTCCATTGTCAATCACCTGGAATTCCTGAGGGATGAGGAGCTGGAAGAAAGGCGAGAGAAGCGCAGGAAACAACTGGCTGAGGAAGAAACAAAGATAACTGATAAAGGCAAAGAAGATAAGGAGAACAGGGATCAGAGTGCACAGTGTACTGCATCTAAGTCAAATGACTCCACTGAACAGAATCTCTCAGATGGGACGCCTATGCCTGACAGCTACCCAACAACCCCATCTTCAACTGATGCAGCTACATCTGAGTCCAAGGAGACCCTTGGCACTCTGCAATCCTCACAACAGCAACCAACACTCCCAACCCCACCAGCTTTGGGAGAGGTTCCTCAGGAGCTGCAGTCTCCAGCTGGAGAAGGGGGCAGCTCTACACAGCTATTGATGCCTGTAGAGCCAGAGGAATTGGGTCCCACAAGGCCAAGTGGGGAAGCAGAAACAACTCAGATGGAGTTATCCCCAGCTCCCACTATAACCTCACTTTCCCCAGAGAGAGCTGAGGATTCTGATGCACTGACGGCTGTCAGCAGTCAGCTAGAAGGCTCTCCTATGGATACAAGCAGCCTGGCTTCCTGTACCTTAGAGGAGGCTGTGGGTGACACTTCAGCAGCTGGCAGTTCTGAGCAGCCCAGAGCAGGCAGCTCCACTCCTGGGGATGCCCCACCAGCTGTGGCGGAAGTGCAAGGCAGGAGTGATGGGTCAGGGGAATCTGCCCAGCCACCTGAGGACAGCTCCCCACCTGCATCCTCTGAGAGCTCTTCCACCAGAGATTCTGCCGTGGCCATTTCTGGAGCAGATTCCCGAGGAATCCTAGAAGGGCCGTTGCCTTCAACAAGCAGTGAAGAAGAAGATCCCCTTGCGGGTATCAGTCTCCCTGAAGGTGTGGACCCCTCTTTTCTGGCTGCCCTGCCTGATGACATCCGTCGGGAAGTTCTACAGAACCAGCTAGGCATTCGTCCACCAACCCGGACTGCCCCCTCCACAAATAGCTCAGCGCCTGCAGTGGTGGGGAATCCTGGTGTGACTGAAGTGAGCCCTGAGTTTCTGGCTGCCCTGCCTCCAGCCATTCAGGAGGAAGTACTGGCACAGCAGAGAGCTGAGCAGCAGCGACGAGAACTAGCACAGAATGCCAGCTCAGACACCCCTATGGACCCTGTGACCTTCATCCAGACTCTGCCCTCAGACCTGCGCCGTAGTGTCCTAGAGGATATGGAGGACAGTGTGTTAGCTGTGATGCCACCTGACATTGCAGCTGAGGCTCAAGCCCTGAGACGAGAGCAAGAAGCCCGGCAGCGACAGCTCATGCATGAGCGTCTGTTTGGGCACAGTAGCACCTCCGCACTCTCTGCTATTCTCCGAAGCCCGGCTTTCACCAGTCGCTTAAGTGGCAACCGTGGGGTCCAGTATACTCGCCTTGCTGTGCAGAGAGGTGGCACCTTCCAGATGGGGGGTAGCAGCAGCCATAACAGGCCTTCTGGCAGTAATGTAGATACTCTCCTCCGCCTCCGAGGACGGCTCCTTCTGGACCACGAAGCCCTTTCTTGTCTCTTGGTCCTACTTTTTGTGGATGAGCCAAAGCTCAATACTAGCCGTCTACACCGAGTACTGAGAAATCTCTGCTACCATGCCCAGACCCGCCACTGGGTCATCCGCAGTCTGCTCTCCATCTTGCAGCGCAGCAGTGAGAGTGAGCTATGCATTGAAACACCCAAACTCACTACAAGTGAGGAAAAGGGCAAAAAGTCGAGCAAGAGCTGTGGGTCAAGTAGCCATGAGAACCGTCCCCTGGACCTGCTACACAAGATGGAGTCAAAGAGCTCCAACCAGCTTTCCTGGCTCTCAGTATCCATGGATGCAGCCCTAGGCTGCAGGACTAATATATTTCAGATCCAGCGTTCAGGGGGGCGTAAACATACCGAGAAGCATGCAAGCGGTGACTCCACCGTCCACATCCATCCCCAAGCTGCACCTGTTGTCTGCAGACACGTTTTGGATACACTCATTCAATTGGCCAAGGTATTTCCCAGCCACTTCACACAGCAGCGGACCAAAGAAACAAACTGTGAGAGTGATCGGGAAAGGGGCAATAAGGCCTGTAGCCCATGCTCCTCACAGTCCTCCAGCAGTGGCATTTGCACAGACTTCTGGGACTTATTGGTAAAACTGGACAACATGAATGTCAGCCGGAAAGGCAAGAACTCCGTGAAGTCAGTGCCAGTGAGCGCTGGCGGTGAGGGGGAAACCTCTCCATACAGCCTCGAGGCCTCTCCACTGGGGCAGCTCATGAACATGTTGTCACACCCAGTCATCCGCCGGAGCTCTCTCTTAACTGAGAAACTCCTCAGACTCCTTTCTCTCATCTCAATTGCTCTCCCAGAAAACAAGGTGTCAGAAGCACAGGCTAATTCTGGCAGCGGTGCTTCCTCCACCACCACTGCCACCTCAACCACATCTACCACCACCACCACTGCCGCCTCCACCACGCCCACACCCCCTACTGCACCCACCCCTGTCACTTCTGCTCCAGCCCTGGTTGCTGCCACGGCTATTTCCACCATTGTCGTAGCTGCTTCGACCACAGTGACTACCCCCACGACTGCTACCACTACTGTTTCAATTTCTCCCACTACTAAGGGCAGCAAATCTCCAGCGAAGGTGAGTGATGGGGGCAGCAGCAGTACAGACTTTAAGATGGTGTCCTCTGGCCTCACTGAAAACCAGCTACAGCTCTCTGTAGAGGTGTTGACATCCCACTCTTGTTCTGAGGAAGGCTTAGAGGATGCAGCCAACGTACTACTGCAGCTCTCCCGGGGGGACTCTGGGACCCGGGACACTGTTCTCAAGCTGCTACTGAATGGAGCCCGCCATCTGGGTTATACCCTTTGTAAACAAATAGGTACCCTGCTGGCCGAGCTGCGGGAATACAACCTCGAGCAGCAGCGGCGAGCCCAATGTGAAACCCTCTCTCCTGATGGCCTGCCTGAGGAGCAGCCACAGACCACCAAGCTGAAGGGCAAAATGCAGAGCAGGTTTGACATGGCTGAGAATGTGGTAATTGTGGCATCTCAGAAGCGACCTTTGGGTGGCCGGGAGCTCCAGCTGCCTTCTATGTCCATGTTGACATCCAAGACATCTACCCAGAAGTTCTTCTTGAGGGTACTACAGGTCATCATCCAGCTCCGGGACGACACGCGCCGGGCTAACAAGAAAGCCAAGCAGACAGGCAGGCTAGGTTCCTCCGGTTTAGGCTCAGCTAGCAGCATCCAGGCAGCTGCTCGGCAGCTGGAGGCTGAGGCTGATGCCATTATACAAATGGTACGTGAGGGTCAAAGGGCGCGGAGACAGCAACAAGCAGCAACGTCGGAGTCTAGCCAGTCAGAGGCGTCTGTCCGGAGGGAGGAATCACCCATGGATGTGGACCAGCCATCTCCCAGTGCTCAAGATACTCAATCCATTGCCTCCGATGGAACCCCACAGGGGGAGAAGGAAAAGGAAGAAAGACCACCTGAGTTACCCCTGCTCAGCGAGCAGCTGAGTTTGGACGAGCTGTGGGACATGCTTGGGGAGTGTCTAAAGGAACTAGAGGAATCCCATGACCAGCATGCGGTGCTAGTGCTACAGCCTGCTGTCGAGGCCTTCTTTCTGGTCCATGCCACAGAGCGGGAGAGCAAGCCTCCTGTCCGAGACACCCGTGAGAGCCAGCTGGCACACATCAAGGACGAGCCTCCTCCACTCTCCCCTGCCCCCTTAACCCCAGCCACGCCTTCCTCCCTTGACCCATTCTTCTCCCGGGAGCCCTCATCTATGCACATCTCCTCAAGCCTGCCCCCTGACACACAGAAGTTCCTTCGCTTTGCAGAGACTCACCGCACTGTGTTAAACCAGATCCTACGGCAGTCCACGACCCACCTTGCTGATGGGCCTTTTGCTGTCCTGGTAGACTACATTCGTGTCCTCGACTTTGATGTCAAGCGCAAATATTTCCGCCAAGAGCTGGAGCGTTTAGATGAGGGGCTCCGGAAAGAAGACATGGCTGTGCATGTCCGTCGTGACCATGTGTTTGAAGACTCCTATCGTGAGCTGCATCGCAAATCCCCCGAAGAAATGAAGAATCGATTGTATATAGTATTTGAAGGAGAAGAAGGGCAGGATGCTGGCGGGCTCCTGCGGGAGTGGTATATGATCATCTCTCGAGAGATGTTTAACCCTATGTATGCCTTGTTCCGTACCTCACCTGGTGATCGAGTCACCTACACCATCAATCCATCTTCCCACTGCAACCCCAACCACCTCAGCTACTTCAAGTTTGTCGGACGCATTGTGGCCAAAGCTGTATATGACAACCGTCTTCTGGAGTGCTACTTTACTCGATCCTTTTACAAACACATCTTGGGCAAGTCAGTCAGATATACAGATATGGAGAGTGAAGATTACCACTTCTACCAAGGTCTGGTTTATCTGCTGGAAAATGATGTCTCCACACTAGGCTATGACCTCACCTTCAGCACTGAGGTCCAAGAGTTTGGAGTTTGTGAAGTTCGTGACCTCAAACCCAATGGGGCCAACATCTTGGTAACAGAGGAGAATAAGAAGGAGTATGTACACCTGGTATGCCAGATGAGAATGACAGGAGCCATCCGCAAGCAGTTGGCGGCTTTCTTAGAAGGCTTCTATGAGATCATTCCAAAGCGCCTCATTTCCATCTTCACTGAGCAGGAGTTAGAGCTGCTTATATCAGGACTGCCCACCATTGACATCGATGATCTGAAATCCAACACTGAATACCACAAGTACCAGTCCAACTCTATTCAGATCCAGTGGTTCTGGAGAGCATTGCGTTCTTTCGATCAAGCTGACCGTGCCAAGTTCCTCCAGTTTGTCACGGGTACTTCCAAGGTACCCCTGCAAGGCTTTGCTGCCCTCGAAGGCATGAATGGCATTCAGAAGTTTCAGATCCATCGAGATGACAGGTCCACAGATCGCCTGCCTTCAGCTCACACATGTTTTAATCAGCTGGATCTGCCTGCCTATGAGAGCTTTGAGAAGCTCCGCCACATGCTACTGTTGGCTATCCAGGAGTGCTCTGAAGGCTTTGGGCTGGCCTAGGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDGVQCIPQRAVLLKSMLNFLKKAIQDPTFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSTQQNETEPNQQVVGTEERIPIPLMDYILNVMKFAESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDSILSSLEPLHRPIESPGGSVLLRELACAGNVAGATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGAAGSMDASTQGLLEGIGLDGDTLAPMETDEPTASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIMGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIVEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSPLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGVMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSINSQVFAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRASEEGKQKGKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSNQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQHEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVCHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQSAQCTASKSNDSTEQNLSDGTPMPDSYPTTPSSTDAATSESKETLGTLQSSQQQPTLPTPPALGEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTSAAGSSEQPRAGSSTPGDAPPAVAEVQGRSDGSGESAQPPEDSSPPASSESSSTRDSAVAISGADSRGILEGPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLTTSEEKGKKSSKSCGSSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASGDSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGNKACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSAGGEGETSPYSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEAQANSGSGASSTTTATSTTSTTTTTAASTTPTPPTAPTPVTSAPALVAATAISTIVVAASTTVTTPTTATTTVSISPTTKGSKSPAKVSDGGSSSTDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAARQLEAEADAIIQMVREGQRARRQQQAATSESSQSEASVRREESPMDVDQPSPSAQDTQSIASDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU66232
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Fragment M1-P337 cut Bgl/BamHI and ligated into 66167 to create final product, GROW AT 30C (14kb insert) Please express GFP-HUWE1 in FRT/TO 5.1 expression ; Changes from NM_031407.6 CDS: C506V A523T V777A D882G I1828V R2521C E2946G G3319D V3765A
• Low Temp: No