cDNA Clone Mindy3/FAM188A

• Synonyms: Derp5, C10orf97; CARP; MINDY3; FAM188A; MST126; MSTP126; my04
• Plasmid: pGEX6P1 Mindy3 F258A
• Parent Plasmid: pGEX6P1
• Expressed: GST-C3-Mindy3 F258A
• Genbank: Q9H8M7
• Tag: GST

Sequence of Insert

GGATCCATGTCCGAACTGACTAAAGAGCTGATGGAGCTGGTGTGGGGCACCAAGAGCAGCCCCGGTCTCTCGGACACCATTTTCTGCCGCTGGACGCAAGGGTTTGTGTTTAGTGAATCAGAGGGATCTGCATTAGAACAGTTTGAAGGTGGCCCCTGTGCTGTTATTGCACCTGTTCAGGCATTTCTTTTGAAGAAGCTCCTGTTTTCTTCGGAGAAGTCTTCTTGGCGGGATTGTTCAGAGGAAGAGCAGAAGGAACTCCTTTGTCATACCTTGTGTGATATTTTAGAAAGTGCTTGTTGTGACCACTCTGGATCATACTGCTTGGTTTCATGGTTAAGAGGAAAGACAACTGAGGAAACTGCTAGTATTTCTGGGAGTCCTGCAGAGTCTAGTTGCCAAGTGGAACATTCTTCTGCCTTGGCTGTCGAAGAGCTTGGCTTTGAGCGATTTCATGCATTAATTCAAAAAAGATCGTTCAGAAGTTTACCAGAATTAAAAGATGCTGTCTTGGACCAGTATTCAATGTGGGGAAATAAATTTGGAGTATTGCTTTTTCTGTATTCTGTATTACTGACAAAGGGCATTGAAAACATAAAAAACGAAATTGAAGATGCAAGTGAACCCTTGATAGATCCTGTATATGGACATGGCAGCCAAAGTTTAATTAATCTCCTGCTGACGGGACATGCTGTTTCTAATGTATGGGATGGTGATAGAGAGTGCTCAGGAATGAAACTTCTTGGTATACATGAACAAGCAGCAGTAGGAGCTTTAACACTAATGGAAGCTTTAAGATACTGTAAGGTTGGTTCTTACTTGAAATCTCCAAAATTCCCTATTTGGATTGTTGGCAGTGAGACTCACCTCACCGTATTTTTTGCCAAGGATATGGCTTTAGTTGCCCCTGAAGCTCCTTCAGAACAAGCCAGAAGAGTTTTTCAAACCTACGACCCAGAAGATAATGGATTCATACCCGATTCACTTCTGGAAGATGTGATGAAAGCATTGGACCTTGTTTCAGATCCTGAATATATAAATCTCATGAAGAATAAATTAGATCCAGAAGGATTAGGAATCATATTATTGGGCCCATTTCTTCAAGAATTTTTTCCTGATCAGGGCTCCAGTGGTCCAGAATCTTTTACTGTCTACCACTACAATGGATTGAAGCAGTCAAATTATAATGAAAAGGTCATGTACGTAGAAGGGACTGCAGTTGTGATGGGTTTTGAAGATCCCATGCTACAGACAGATGACACTCCTATTAAACGCTGTCTGCAAACCAAATGGCCATACATTGAGTTACTCTGGACCACAGATCGCTCTCCTTCACTAAATTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSELTKELMELVWGTKSSPGLSDTIFCRWTQGFVFSESEGSALEQFEGGPCAVIAPVQAFLLKKLLFSSEKSSWRDCSEEEQKELLCHTLCDILESACCDHSGSYCLVSWLRGKTTEETASISGSPAESSCQVEHSSALAVEELGFERFHALIQKRSFRSLPELKDAVLDQYSMWGNKFGVLLFLYSVLLTKGIENIKNEIEDASEPLIDPVYGHGSQSLINLLLTGHAVSNVWDGDRECSGMKLLGIHEQAAVGALTLMEALRYCKVGSYLKSPKFPIWIVGSETHLTVFFAKDMALVAPEAPSEQARRVFQTYDPEDNGFIPDSLLEDVMKALDLVSDPEYINLMKNKLDPEGLGIILLGPFLQEFFPDQGSSGPESFTVYHYNGLKQSNYNEKVMYVEGTAVVMGFEDPMLQTDDTPIKRCLQTKWPYIELLWTTDRSPSLN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU66070
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please mutate F258 in 47870 to alanine. Thanks!
• Low Temp: No