cDNA Clone HERC4

• Plasmid: pCMV5 HA DU-HERC4 isoform b delta R843
• Parent Plasmid: pCMV5 HA DU
• Expressed: HA-HERC4 isoform b delta R843
• Genbank: NM_015601.3 → NP_056416.2
• Tag: HA
• Species: Human

Sequence of Insert

gaattcATGTTGTGCTGGGGAAATGCATCCTTTGGGCAGCTAGGTTTGGG
TGGAATTGATGAAGAAATTGTACTAGAGCCCAGAAAAAGTGACTTCTTTA
TAAATAAAAGGGTCCGAGATGTAGGATGTGGACTCAGACATACTGTGTTT
GTTCTGGATGATGGAACAGTGTACACATGTGGATGTAATGATCTAGGACA
GCTAGGTCATGAAAAATCCAGAAAGAAACCAGAGCAGGTTGTTGCCCTGG
ATGCCCAAAATATTGTAGCTGTTTCATGTGGAGAAGCTCATACGTTAGCG
CTAAATGACAAAGGCCAGGTGTATGCTTGGGGTCTCGATTCTGATGGACA
GCTTGGCCTGGTAGGATCAGAGGAATGCATCAGAGTACCCAGAAATATTA
AAAGTTTGTCAGATATCCAGATTGTACAGGTTGCTTGTGGTTACTATCAT
TCACTTGCACTTTCTAAAGCAAGTGAAGTCTTCTGTTGGGGACAGAATAA
ATATGGCCAATTGGGTTTAGGTACTGACTGTAAAAAGCAAACTTCACCGC
AGCTGCTTAAGTCTTTGCTTGGAATCCCTTTCATGCAAGTTGCAGCAGGA
GGAGCCCATAGTTTTGTACTCACCCTTTCTGGAGCTATCTTTGGATGGGG
ACGCAACAAGTTTGGTCAGCTAGGTCTTAATGATGAAAATGATAGGTATG
TTCCTAATTTACTAAAGTCACTAAGATCTCAGAAAATAGTTTATATTTGT
TGTGGAGAAGATCATACTGCTGCTCTAACCAAGGAAGGTGGAGTGTTTAC
TTTTGGAGCTGGAGGGTATGGTCAGTTGGGCCATAATTCTACCAGTCATG
AAATAAACCCAAGGAAAGTTTTTGAACTTATGGGAAGCATTGTCACTGAG
ATTGCTTGTGGACGGCAGCACACTTCTGCTTTTGTTCCTTCATCAGGACG
AATTTACTCTTTTGGGCTTGGTGGTAATGGGCAGCTGGGAACCGGTTCAA
CAAGCAACAGGAAAAGCCCCTTTACTGTAAAAGGAAATTGGTACCCCTAT
AATGGGCAGTGTCTACCAGATATTGATTCTGAAGAATATTTCTGTGTGAA
AAGAATTTTCTCAGGGGGAGATCAAAGCTTTTCACATTACTCTAGTCCCC
AGAACTGTGGGCCACCAGATGACTTCAGATGTCCCAATCCGACAAAGCAG
ATCTGGACAGTGAATGAAGCTCTAATTCAGAAATGGCTGAGCTATCCTTC
TGGAAGGTTTCCTGTGGAGATAGCCAATGAGATAGATGGAACGTTTTCTT
CCTCTGGTTGCCTAAATGGAAGTTTTTTAGCTGTTAGCAATGATGATCAC
TATAGAACAGGTACCAGATTTTCAGGGGTTGATATGAATGCTGCTAGGCT
TTTATTCCACAAACTTATACAACCTGATCATCCGCAGATATCTCAGCAGG
TGGCAGCTAGTTTGGAAAAGAATCTTATTCCTAAACTGACTAGCTCCTTA
CCTGATGTTGAAGCATTGAGGTTTTATCTTACTCTACCAGAATGTCCCCT
GATGAGTGATTCCAACAATTTCACAACAATAGCAATTCCCTTTGGTACAG
CTCTTGTGAACCTAGAAAAGGCACCACTGAAAGTACTTGAAAACTGGTGG
TCAGTACTTGAACCTCCACTATTCCTCAAGATAGTAGAACTTTTTAAGGA
AGTTGTGGTACATCTTTTGAAACTCTACAAGATCGGTATTCCCCCTTCTG
AAAGAAGAATTTTCAACAGTTTTCTTCATACTGCATTAAAGGTTTTAGAA
ATACTACATAGGGTAAATGAGAAAATGGGACAGATTATACAGTATGATAA
ATTTTATATACATGAAGTACAAGAATTGATAGACATAAGAAATGATTATA
TCAACTGGGTCCAACAGCAGGCCTATGGAATGTTGGCAGATATCCCTGTT
ACAATCTGTACATATCCATTTGTATTTGATGCCCAAGCAAAAACTACTCT
GTTACAGACCGATGCAGTCTTACAGATGCAGATGGCTATTGATCAGGCCC
ACAGGCAGAATGTCTCCTCTCTTTTTCTCCCAGTGATTGAATCTGTGAAT
CCCTGCTTAATTCTAGTGGTGCGTAGAGAAAATATTGTAGGAGATGCAAT
GGAAGTCCTTAGGAAAACAAAGAACATAGATTACAAGAAGCCACTCAAGG
TTATATTTGTTGGAGAAGATGCTGTGGATGCAGGAGGGGTGCGCAAAGAA
TTTTTCTTGCTCATCATGAGGGAATTATTGGATCCTAAATACGGCATGTT
TAGGTATTATGAAGATTCCAGGCTCATTTGGTTTTCTGATAAGACATTTG
AAGACAGTGATTTGTTCCATTTGATTGGTGTTATCTGTGGCTTAGCAATT
TATAATTGTACCATTGTGGACCTCCATTTTCCTTTGGCTTTATATAAGAA
ACTACTGAAAAAGAAGCCATCCTTGGATGATTTGAAAGAACTAATGCCTG
ATGTTGGGAGCATGCAACAGTTACTGGATTATCCAGAAGATGACATAGAG
GAAACATTTTGTCTTAATTTTACGATCACAGTTGAAAACTTTGGTGCAAC
AGAAGTGAAAGAGCTGGTTCTAAATGGTGCAGACACAGCTGTTAACAAAC
AAAATCGGCAAGAGTTTGTCGATGCTTATGTGGATTACATATTCAATAAA
TCAGTGGCTTCCTTATTTGATGCTTTTCATGCGGGCTTTCATAAGGTCTG
TGGAGGAAAAGTCCTTCTGCTCTTTCAGCCTAATGAACTACAAGCAATGG
TCATTGGAAATACAAATTATGATTGGAAGGAACTGGAAAAGAATACAGAA
TACAAAGGGGAATATTGGGCAGAACATCCTACGATAAAAATTTTTTGGGA
AGTATTTCACGAATTACCATTGGAAAAGAAGAAACAGTTTCTGTTATTTT
TGACAGGTAGTGATCGCATTCCTATTCTTGGTATGAAGAGTCTGAAACTA
GTCATCCAGTCCACAGGAGGTGGTGAGGAGTATCTCCCAGTTTCCCATAC
TTGTTTTAATCTTCTGGATCTTCCAAAATATACAGAAAAAGAAACTCTAC
GCTCTAAACTGATCCAAGCTATTGATCACAATGAAGGCTTCAGTTTAATA
TAAgcggccgc

Amino Acid Sequence

MYPYDVPDYAGSFEEFMLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINK
RVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQ
NIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLVGSEECIRVPRNIKSL
SDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTSPQLL
KSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPN
LLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEIN
PRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSN
RKSPFTVKGNWYPYNGQCLPDIDSEEYFCVKRIFSGGDQSFSHYSSPQNC
GPPDDFRCPNPTKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSG
CLNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAA
SLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALV
NLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERR
IFNSFLHTALKVLEILHRVNEKMGQIIQYDKFYIHEVQELIDIRNDYINW
VQQQAYGMLADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQ
NVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIF
VGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDS
DLFHLIGVICGLAIYNCTIVDLHFPLALYKKLLKKKPSLDDLKELMPDVG
SMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNR
QEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIG
NTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTG
SDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSK
LIQAIDHNEGFSLI*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65293
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Isoform b. silent change compared to NCBI ref at V364 (GTA > GTG) and is also missing R843; ; Isoform b contains a deletion of D643-E650 compared to isoform a (NM_022079.2); Parental 44059
• Low Temp: No