cDNA Clone TEV Protease

• Plasmid: pET15A TEV Nla Protease S219V
• Parent Plasmid: pET15a
• Expressed: 10His TEV Nla Protease S219V
• Genbank: NP_734212
• Tag: 6His
• Species: Tobacco Etch Virus

Sequence of Insert

CTCGAGAGCTTGTTTAAGGGACCACGTGATTACAACCCGATATCGAGCACCATTTGTCATTTGACGAATGAATCTGATGGGCACACAACATCGTTGTATGGTATTGGATTTGGTCCCTTCATCATTACAAACAAGCACTTGTTTAGAAGAAATAATGGAACACTGTTGGTCCAATCACTACATGGTGTATTCAAGGTCAAGAACACCACGACTTTGCAACAACACCTCATTGATGGGAGGGACATGATAATTATTCGCATGCCTAAGGATTTCCCACCATTTCCTCAAAAGCTGAAATTTAGAGAGCCACAAAGGGAAGAGCGCATATGTCTTGTGACAACCAACTTCCAAACTAAGAGCATGTCTAGCATGGTGTCAGACACTAGTTGCACATTCCCTTCATCTGATGGCATATTCTGGAAGCATTGGATTCAAACCAAGGATGGGCAGTGTGGCAGTCCATTAGTATCAACTAGAGATGGGTTCATTGTTGGTATACACTCAGCATCGAATTTCACCAACACAAACAATTATTTCACAAGCGTGCCGAAAAACTTCATGGAATTGTTGACAAATCAGGAGGCGCAGCAGTGGGTTAGTGGTTGGCGATTAAATGCTGACTCAGTATTGTGGGGGGGCCATAAAGTTTTCATGGTCAAACCTGAAGAGCCTTTTCAGCCAGTTAAGGAAGCGACTCAACTCATGAATGAATTGGTGTACTCGCAATAAGGATCC

Amino Acid Sequence

MGHHHHHHHHHHSSGHIDDDDKHMLESLFKGPRDYNPISSTICHLTNESD
GHTTSLYGIGFGPFIITNKHLFRRNNGTLLVQSLHGVFKVKNTTTLQQHL
IDGRDMIIIRMPKDFPPFPQKLKFREPQREERICLVTTNFQTKSMSSMVS
DTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFIVGIHSASNFTNTN
NYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMVKPEEPFQ
PVKEATQLMNELVYSQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU65254
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: S219V to prevent self-proteolysis; N-terminal 10His tag; The Nla-Pro protein (NP_734212.1) represents Gly2038-Q2279 of the polyprotein NP_062908.1
• Low Temp: No