cDNA Clone TEV Protease

• Plasmid: pcDNA5 FRT/TO-TEV Nla Protease S219V
• Parent Plasmid: pcDNA5 FRT/TO
• Expressed: TEV Nla Protease S219V
• Genbank: NP_734212
• Species: Tobacco Etch Virus

Sequence of Insert

ggatccATGggCGAGAGCTTGTTTAAGGGACCACGTGATTACAACCCGAT
ATCGAGCACCATTTGTCATTTGACGAATGAATCTGATGGGCACACAACAT
CGTTGTATGGTATTGGATTTGGTCCCTTCATCATTACAAACAAGCACTTG
TTTAGAAGAAATAATGGAACACTGTTGGTCCAATCACTACATGGTGTATT
CAAGGTCAAGAACACCACGACTTTGCAACAACACCTCATTGATGGGAGGG
ACATGATAATTATTCGCATGCCTAAGGATTTCCCACCATTTCCTCAAAAG
CTGAAATTTAGAGAGCCACAAAGGGAAGAGCGCATATGTCTTGTGACAAC
CAACTTCCAAACTAAGAGCATGTCTAGCATGGTGTCAGACACTAGTTGCA
CATTCCCTTCATCTGATGGCATATTCTGGAAGCATTGGATTCAAACCAAG
GATGGGCAGTGTGGCAGTCCATTAGTATCAACTAGAGATGGGTTCATTGT
TGGTATACACTCAGCATCGAATTTCACCAACACAAACAATTATTTCACAA
GCGTGCCGAAAAACTTCATGGAATTGTTGACAAATCAGGAGGCGCAGCAG
TGGGTTAGTGGTTGGCGATTAAATGCTGACTCAGTATTGTGGGGGGGCCA
TAAAGTTTTCATGgtCAAACCTGAAGAGCCTTTTCAGCCAGTTAAGGAAG
CGACTCAACTCATGAATGAATTGGTGTACTCGCAATAAgcggccgc

Amino Acid Sequence

MGESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNKHLFR
RNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLK
FREPQREERICLVTTNFQTKSMSSMVSDTSCTFPSSDGIFWKHWIQTKDG
QCGSPLVSTRDGFIVGIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQWV
SGWRLNADSVLWGGHKVFMVKPEEPFQPVKEATQLMNELVYSQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65263
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: S219V to prevent self-proteolysis; The Nla-Pro protein (NP_734212.1) represents Gly2038-Q2279 of the polyprotein NP_062908.1; Parental 41459
• Low Temp: No