cDNA Clone Ubiquitin

• Plasmid: pTXB1 Ubiquitin (Met1-Leu73) TAG31 -T3C Mxe intein/chitin binding domain
• Parent Plasmid: pTXB-1
• Expressed: TAG31 Ubiquitin 1-73-T3C Mxe intein/chitin binding domain
• Genbank: P62988
• Tag: Intein
• Species: Human

Sequence of Insert

CATATGCAGATCTTCGTCAAGACGTTAACCGGTAAAACCATAACTCTAGAAGTGGAACCGAGCGATACCATCGAAAACGTGAAAGCGAAAATCTAGGATAAAGAAGGCATCCCGCCGGATCAGCAGCGTCTGATCTTTGCGGGCAAACAGCTGGAAGATGGCCGCACCCTGTCTGATTATAACATCCAGAAAGAGTCGACCCTGCATCTGGTCTTAAGACTGTGCATCTGCGGAGATGCACTAGTTGCCCTACCCGAGGGCGAGTCGGTACGCATCGCCGACATCGTGCCGGGTGCGCGGCCCAACAGTGACAACGCCATCGACCTGAAAGTCCTTGACCGGCATGGCAATCCCGTGCTCGCCGACCGGCTGTTCCACTCCGGCGAGCATCCGGTGTACACGGTGCGTACGGTCGAAGGTCTGCGTGTGACGGGCACCGCGAACCACCCGTTGTTGTGTTTGGTCGACGTCGCCGGGGTGCCGACCCTGCTGTGGAAGCTGATCGACGAAATCAAGCCGGGCGATTACGCGGTGATTCAACGCAGCGCATTCAGCGTCGACTGTGCAGGTTTTGCCCGCGGGAAACCCGAATTTGCGCCCACAACCTACACAGTCGGCGTCCCTGGACTGGTGCGTTTCTTGGAAGCACACCACCGAGACCCGGACGCCCAAGCTATCGCCGACGAGCTGACCGACGGGCGGTTCTACTACGCGAAAGTCGCCAGTGTCACCGACGCCGGCGTGCAGCCGGTGTATAGCCTTCGTGTCGACACGGCAGACCACGCGTTTATCACGAACGGGTTCGTCAGCCACGCTACTGGCCTCACCGGTCTGAACTCAGGCCTCACGACAAATCCTGGTGTATCCGCTTGGCAGGTCAACACAGCTTATACTGCGGGACAATTGGTCACATATAACGGCAAGACGTATAAATGTTTGCAGCCCCACACCTCCTTGGCAGGATGGGAACCATCCAACGTTCCTGCCTTGTGGCAGCTTCAATGACTGCAG

Amino Acid Sequence

MQIFVKTLTGKTITLEVEPSDTIENVKAKI*DKEGIPPDQQRLIFAGKQL
EDGRTLSDYNIQKESTLHLVLRLCICGDALVALPEGESVRIADIVPGARP
NSDNAIDLKVLDRHGNPVLADRLFHSGEHPVYTVRTVEGLRVTGTANHPL
LCLVDVAGVPTLLWKLIDEIKPGDYAVIQRSAFSVDCAGFARGKPEFAPT
TYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYYAKVASVTDAGVQPV
YSLRVDTADHAFITNGFVSHATGLTGLNSGLTTNPGVSAWQVNTAYTAGQ
LVTYNGKTYKCLQPHTSLAGWEPSNVPALWQLQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU65282
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Ubiquitin sequence is codon-optimised. Obtained by Satpal Virdee from outwith MRCPPU. NOT FOR REDISTRIBUTION.
• Low Temp: No