cDNA Clone HERC4

• Plasmid: pcDNA5-FRT/TO-HA-HERC4 isoform b
• Parent Plasmid: pcDNA5-FRT/TO-HA
• Expressed: HA-HERC4 isoform b
• Genbank: NM_015601.3 → NP_056416.2
• Tag: HA
• Species: Human

Sequence of Insert

gaattcATGTTGTGCTGGGGAAATGCATCCTTTGGGCAGCTAGGTTTGGG
TGGAATTGATGAAGAAATTGTACTAGAGCCCAGAAAAAGTGACTTCTTTA
TAAATAAAAGGGTCCGAGATGTAGGATGTGGACTCAGACATACTGTGTTT
GTTCTGGATGATGGAACAGTGTACACATGTGGATGTAATGATCTAGGACA
GCTAGGTCATGAAAAATCCAGAAAGAAACCAGAGCAGGTTGTTGCCCTGG
ATGCCCAAAATATTGTAGCTGTTTCATGTGGAGAAGCTCATACGTTAGCG
CTAAATGACAAAGGCCAGGTGTATGCTTGGGGTCTCGATTCTGATGGACA
GCTTGGCCTGGTAGGATCAGAGGAATGCATCAGAGTACCCAGAAATATTA
AAAGTTTGTCAGATATCCAGATTGTACAGGTTGCTTGTGGTTACTATCAT
TCACTTGCACTTTCTAAAGCAAGTGAAGTCTTCTGTTGGGGACAGAATAA
ATATGGCCAATTGGGTTTAGGTACTGACTGTAAAAAGCAAACTTCACCGC
AGCTGCTTAAGTCTTTGCTTGGAATCCCTTTCATGCAAGTTGCAGCAGGA
GGAGCCCATAGTTTTGTACTCACCCTTTCTGGAGCTATCTTTGGATGGGG
ACGCAACAAGTTTGGTCAGCTAGGTCTTAATGATGAAAATGATAGGTATG
TTCCTAATTTACTAAAGTCACTAAGATCTCAGAAAATAGTTTATATTTGT
TGTGGAGAAGATCATACTGCTGCTCTAACCAAGGAAGGTGGAGTGTTTAC
TTTTGGAGCTGGAGGGTATGGTCAGTTGGGCCATAATTCTACCAGTCATG
AAATAAACCCAAGGAAAGTTTTTGAACTTATGGGAAGCATTGTCACTGAG
ATTGCTTGTGGACGGCAGCACACTTCTGCTTTTGTTCCTTCATCAGGACG
AATTTACTCTTTTGGGCTTGGTGGTAATGGGCAGCTGGGAACCGGTTCAA
CAAGCAACAGGAAAAGCCCCTTTACTGTAAAAGGAAATTGGTACCCCTAT
AATGGGCAGTGTCTACCAGATATTGATTCTGAAGAATATTTCTGTGTAAA
AAGAATTTTCTCAGGGGGAGATCAAAGCTTTTCACATTACTCTAGTCCCC
AGAACTGTGGGCCACCAGATGACTTCAGATGTCCCAATCCGACAAAGCAG
ATCTGGACAGTGAATGAAGCTCTAATTCAGAAATGGCTGAGCTATCCTTC
TGGAAGGTTTCCTGTGGAGATAGCCAATGAGATAGATGGAACGTTTTCTT
CCTCTGGTTGCCTAAATGGAAGTTTTTTAGCTGTTAGCAATGATGATCAC
TATAGAACAGGTACCAGATTTTCAGGGGTTGATATGAATGCTGCTAGGCT
TTTATTCCACAAACTTATACAACCTGATCATCCGCAGATATCTCAGCAGG
TGGCAGCTAGTTTGGAAAAGAATCTTATTCCTAAACTGACTAGCTCCTTA
CCTGATGTTGAAGCATTGAGGTTTTATCTTACTCTACCAGAATGTCCCCT
GATGAGTGATTCCAACAATTTCACAACAATAGCAATTCCCTTTGGTACAG
CTCTTGTGAACCTAGAAAAGGCACCACTGAAAGTACTTGAAAACTGGTGG
TCAGTACTTGAACCTCCACTATTCCTCAAGATAGTAGAACTTTTTAAGGA
AGTTGTGGTACATCTTTTGAAACTCTACAAGATCGGTATTCCCCCTTCTG
AAAGAAGAATTTTCAACAGTTTTCTTCATACTGCATTAAAGGTTTTAGAA
ATACTACATAGGGTAAATGAGAAAATGGGACAGATTATACAGTATGATAA
ATTTTATATACATGAAGTACAAGAATTGATAGACATAAGAAATGATTATA
TCAACTGGGTCCAACAGCAGGCCTATGGAATGTTGGCAGATATCCCTGTT
ACAATCTGTACATATCCATTTGTATTTGATGCCCAAGCAAAAACTACTCT
GTTACAGACCGATGCAGTCTTACAGATGCAGATGGCTATTGATCAGGCCC
ACAGGCAGAATGTCTCCTCTCTTTTTCTCCCAGTGATTGAATCTGTGAAT
CCCTGCTTAATTCTAGTGGTGCGTAGAGAAAATATTGTAGGAGATGCAAT
GGAAGTCCTTAGGAAAACAAAGAACATAGATTACAAGAAGCCACTCAAGG
TTATATTTGTTGGAGAAGATGCTGTGGATGCAGGAGGGGTGCGCAAAGAA
TTTTTCTTGCTCATCATGAGGGAATTATTGGATCCTAAATACGGCATGTT
TAGGTATTATGAAGATTCCAGGCTCATTTGGTTTTCTGATAAGACATTTG
AAGACAGTGATTTGTTCCATTTGATTGGTGTTATCTGTGGCTTAGCAATT
TATAATTGTACCATTGTGGACCTCCATTTTCCTTTGGCTTTATATAAGAA
ACTACTGAAAAAGAAGCCATCCTTGGATGATTTGAAAGAACTAATGCCTG
ATGTTGGGAGAAGCATGCAACAGTTACTGGATTATCCAGAAGATGACATA
GAGGAAACATTTTGTCTTAATTTTACGATCACAGTTGAAAACTTTGGTGC
AACAGAAGTGAAAGAGCTGGTTCTAAATGGTGCAGACACAGCTGTTAACA
AACAAAATCGGCAAGAGTTTGTCGATGCTTATGTGGATTACATATTCAAT
AAATCAGTGGCTTCCTTATTTGATGCTTTTCATGCGGGCTTTCATAAGGT
CTGTGGAGGAAAAGTCCTTCTGCTCTTTCAGCCTAATGAACTACAAGCAA
TGGTCATTGGAAATACAAATTATGATTGGAAGGAACTGGAAAAGAATACA
GAATACAAAGGGGAATATTGGGCAGAACATCCTACGATAAAAATTTTTTG
GGAAGTATTTCACGAATTACCATTGGAAAAGAAGAAACAGTTTCTGTTAT
TTTTGACAGGTAGTGATCGCATTCCTATTCTTGGTATGAAGAGTCTGAAA
CTAGTCATCCAGTCCACAGGAGGTGGTGAGGAGTATCTCCCAGTTTCCCA
TACTTGTTTTAATCTTCTGGATCTTCCAAAATATACAGAAAAAGAAACTC
TACGCTCTAAACTGATCCAAGCTATTGATCACAATGAAGGCTTCAGTTTA
ATATAAgcggccgc

Amino Acid Sequence

MYPYDVPDYAGSPGEFMLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINK
RVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQ
NIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLVGSEECIRVPRNIKSL
SDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTSPQLL
KSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPN
LLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEIN
PRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSN
RKSPFTVKGNWYPYNGQCLPDIDSEEYFCVKRIFSGGDQSFSHYSSPQNC
GPPDDFRCPNPTKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSG
CLNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAA
SLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALV
NLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERR
IFNSFLHTALKVLEILHRVNEKMGQIIQYDKFYIHEVQELIDIRNDYINW
VQQQAYGMLADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQ
NVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIF
VGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDS
DLFHLIGVICGLAIYNCTIVDLHFPLALYKKLLKKKPSLDDLKELMPDVG
RSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQN
RQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVI
GNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLT
GSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRS
KLIQAIDHNEGFSLI*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65294
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Isoform b. No changes compared to NCBI ref WT; Isoform b contains a deletion of D643-E650 compared to isoform a (NM_022079.2); Parental 41456
• Low Temp: No