cDNA Clone Ubiquitin

• Plasmid: pET28a DAC TEV Ubiquitin K48R K63R
• Parent Plasmid: pET28a DAC TEV
• Expressed: Dac-TEV-Ubiquitin [K48R] [K63R]
• Genbank: NM_021009.7
• Tag: DAC
• Species: Human

Sequence of Insert

GGATCCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAGACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAGAGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGGTGGGTAGTGAGTCGAC

Amino Acid Sequence

MSVPGVPQIDAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGNDAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVGLMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFTFNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKGREAESKKLLTWGFRFFETVNPENLYFQGGSMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGRQLEDGRTLSDYNIQRESTLHLVLRLRGG*

• Vector Type: Bacterial
• Antibiotic: Kan
• Full Snapgene Map: pET28a DAC TEV Ubiquitin K48R K63R57.04 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU66305
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 66294 mutated with MP1758/9
• Low Temp: No