cDNA Clone HOIL1

• Plasmid: pCMV-HA-1-HOIL1 (M1-G478)
• Parent Plasmid: pCMV-HA-1
• Expressed: HA-HOIL1 (M1-G478)
• Genbank: NM_031229
• Tag: HA
• Species: Human

Sequence of Insert

gaattcATGGACGAGAAGACCAAGAAAGCAGAGGAAATGGCCCTGAGCCT
CACCCGAGCAGTGGCGGGCGGGGATGAACAGGTGGCAATGAAGTGTGCCA
TCTGGCTGGCAGAGCAACGGGTGCCCCTGAGTGTGCAACTGAAGCCTGAG
GTCTCCCCAACGCAGGACATCAGGCTGTGGGTGAGCGTGGAGGATGCTCA
GATGCACACCGTCACCATCTGGCTCACAGTGCGCCCTGATATGACAGTGG
CGTCTCTCAAGGACATGGTTTTTCTGGACTATGGCTTCCCACCAGTCTTG
CAGCAGTGGGTGATTGGGCAGCGGCTGGCACGAGACCAGGAGACCCTGCA
CTCCCATGGGGTGCGGCAGAATGGGGACAGTGCCTACCTCTATCTGCTGT
CAGCCCGCAACACCTCCCTCAACCCTCAGGAGCTGCAGCGGGAGCGGCAG
CTGCGGATGCTGGAAGATCTGGGCTTCAAGGACCTCACGCTGCAGCCGCG
GGGCCCTCTGGAGCCAGGCCCCCCAAAGCCCGGGGTCCCCCAGGAACCCG
GACGGGGGCAGCCAGATGCAGTGCCTGAGCCCCCACCGGTGGGCTGGCAG
TGCCCCGGGTGCACCTTCATCAACAAGCCCACGCGGCCTGGCTGTGAGAT
GTGCTGCCGGGCGCGCCCCGAGGCCTACCAGGTCCCCGCCTCATACCAGC
CCGACGAGGAGGAGCGAGCGCGCCTGGCGGGCGAGGAGGAGGCGCTGCGT
CAGTACCAGCAGCGGAAGCAGCAGCAGCAGGAGGGGAACTACCTGCAGCA
CGTCCAGCTGGACCAGAGGAGCCTGGTGCTGAACACGGAGCCCGCCGAGT
GCCCCGTGTGCTACTCGGTGCTGGCGCCCGGCGAGGCCGTGGTGCTGCGT
GAGTGTCTGCACACCTTCTGCAGGGAGTGCCTGCAGGGCACCATCCGCAA
CAGCCAGGAGGCGGAGGTCTCCTGCCCCTTCATTGACAACACCTACTCGT
GCTCGGGCAAGCTGCTGGAGAGGGAGATCAAGGCGCTCCTGACCCCTGAG
GATTACCAGCGATTTCTAGACCTGGGCATCTCCATTGCTGAAAACCGCAG
TGCCTTCAGCTACCATTGCAAGACCCCAGATTGCAAGGGATGGTGCTTCT
TTGAGGATGATGTCAATGAGTTCACCTGCCCTGTGTGTTTCCACGTCAAC
TGCCTGCTCTGCAAGGCCATCCATGAGCAGATGAACTGCAAGGAGTATCA
GGAGGACCTGGCCCTGCGGGCTCAGAACGATGTGGCTGCCCGGCAGACGA
CAGAGATGCTGAAGGTGATGCTGCAGCAGGGCGAGGCCATGCGCTGCCCC
CAGTGCCAGATCGTGGTACAGAAGAAGGACGGCTGCGACTGGATCCGCTG
CACCGTCTGCCACACCGAGATCTGCTGGGTCACCAAGGGCtgagcggccg
c

Amino Acid Sequence

MYPYDVPDYAGSPEFMDEKTKKAEEMALSLTRAVAGGDEQVAMKCAIWLA
EQRVPLSVQLKPEVSPTQDIRLWVSVEDAQMHTVTIWLTVRPDMTVASLK
DMVFLDYGFPPVLQQWVIGQRLARDQETLHSHGVRQNGDSAYLYLLSARN
TSLNPQELQRERQLRMLEDLGFKDLTLQPRGPLEPGPPKPGVPQEPGRGQ
PDAVPEPPPVGWQCPGCTFINKPTRPGCEMCCRARPEAYQVPASYQPDEE
ERARLAGEEEALRQYQQRKQQQQEGNYLQHVQLDQRSLVLNTEPAECPVC
YSVLAPGEAVVLRECLHTFCRECLQGTIRNSQEAEVSCPFIDNTYSCSGK
LLEREIKALLTPEDYQRFLDLGISIAENRSAFSYHCKTPDCKGWCFFEDD
VNEFTCPVCFHVNCLLCKAIHEQMNCKEYQEDLALRAQNDVAARQTTEML
KVMLQQGEAMRCPQCQIVVQKKDGCDWIRCTVCHTEICWVTKG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65413
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Truncation. N-term HA tag. Mammalian expression.
• Low Temp: No