cDNA Clone ZNF609

• Plasmid: pcDNA5D-FRT/TO HA-ZNF609
• Parent Plasmid: pcDNA5D-FRT/TO HA
• Expressed: HA-ZNF609
• Genbank: NM_015042.2 → NP_055857.1 AC090543.12 (Uniprot O15014)
• Tag: HA
• Species: Human

Sequence of Insert

ctcgagATGTCCTTGAGCAGTGGAGCCTCCGGAGGGAAAGGAGTGGATGC
AAACCCGGTTGAGACATACGACAGTGGGGATGAATGGGACATTGGAGTAG
GGAATCTCATCATTGACCTGGACGCCGATCTGGAAAAGGACCAGCAGAAA
CTGGAAATGTCAGGCTCAAAGGAGGTGGGGATACCGGCTCCCAATGCTGT
GGCCACACTACCAGACAACATCAAGTTTGTGACCCCAGTGCCAGGTCCTC
AAGGGAAGGAAGGCAAATCAAAATCCAAAAGGAGTAAGAGTGGCAAAGAC
ACTAGCAAACCCACTCCAGGGACTTCCCTGTTCACTCCAAGTGAGGGGGC
AGCTAGCAAGAAAGAGGTGCAGGGGCGCTCAGGAGATGGTGCCAATGCTG
GAGGCCTGGTTGCTGCTATTGCTCCCAAGGGCTCAGAGAAGGCGGCTAAG
GCATCCCGCAGTGTAGCCGGTTCCAAAAAGGAGAAGGAGAACAGCTCATC
TAAGAGCAAGAAGGAGAGAAGCGAAGGAGTGGGGACTTGTTCAGAAAAGG
ATCCTGGGGTCCTCCAGCCAGTTCCCTTGGGAGGACGGGGTGGTCAGTAT
GATGGAAGTGCAGGGGTGGATACAGGAGCTGTGGAGCCACTTGGGAGTAT
AGCTATTGAGCCTGGGGCAGCGCTCAATCCTTTGGGAACTAAACCGGAGC
CAGAGGAAGGGGAGAATGAGTGTCGCCTGCTAAAGAAAGTCAAGTCTGAA
AAGATGGAGTCCCCTGTTTCCACACCAGCAGTGCTGCCAATACACCTTTT
GGTGCCAGTGGTCAACAATGACATCTCATCTCCTTGTGAGCAGATCATGG
TTCGTACCCGATCAGTTGGGGTCAACACATGTGATGTGGCTCTGGCCACA
GAGCCTGAGTGCTTGGGCCCCTGTGAACCTGGAACTAGCGTCAACCTTGA
AGGCATCGTGTGGCAGGAAACAGAAGATGGGATGTTGGTGGTAAATGTAA
CGTGGAGGAACAAGACATATGTAGGTACACTCCTTGACTGCACACGACAT
GATTGGGCACCCCCAAGGTTCTGTGACTCCCCGACCAGTGACCTGGAAAT
GCGCAATGGCCGGGGTAGAGGCAAACGCATGCGTCCCAACAGTAATACAC
CTGTCAATGAGACAGCCACAGCCTCTGACAGCAAAGGGACCAGTAACAGC
AGCAAAACCCGGGCAGGAGCCAATAGCAAAGGCCGTCGGGGCAGCCAGAA
TTCTTCAGAGCACCGCCCACCTGCCAGCAGCACTTCTGAGGATGTCAAGG
CCAGCCCTTCCTCAGCTAATAAGCGGAAAAACAAACCCCTTTCAGACATG
GAGCTGAATTCTAGCTCAGAGGACTCCAAAGGGAGCAAGCGTGTCCGTAC
TAATTCCATGGGCTCAGCCACTGGCCCCCTTCCTGGGACAAAGGTAGAAC
CCACTGTTCTGGACAGAAACTGCCCCTCCCCCGTCCTAATTGACTGTCCC
CACCCAAACTGCAACAAGAAGTACAAGCACATCAATGGACTTAAGTACCA
CCAAGCTCATGCCCATACAGATGATGACAGCAAGCCGGAAGCGGATGGGG
ACAGTGAGTACGGAGAGGAACCTATTCTCCATGCAGATCTTGGGAGCTGC
AACGGTGCATCTGTCTCACAAAAAGGTTCCTTGTCCCCTGCCCGCTCAGC
TACCCCCAAAGTTCGACTTGTAGAGCCCCATAGCCCTTCTCCTTCAAGCA
AATTCAGCACAAAAGGCCTCTGTAAGAAAAAGTTGAGTGGGGAAGGGGAC
ACAGACCTTGGGGCCTTATCCAATGATGGCTCTGATGATGGACCCTCAGT
GATGGATGAAACAAGCAATGATGCCTTTGATTCTTTAGAAAGGAAGTGTA
TGGAAAAAGAAAAATGTAAAAAACCCTCTAGTTTAAAACCTGAAAAGATT
CCTTCCAAGAGCCTAAAGTCAGCCCGTCCCATTGCCCCTGCCATCCCCCC
ACAGCAAATCTACACCTTCCAGACAGCCACCTTCACAGCAGCGAGCCCAG
GCTCTTCCTCAGGCTTGACCGCCACAGTGGCACAAGCCATGCCCAACAGT
CCCCAACTCAAGCCCATTCAGCCCAAGCCCACTGTTATGGGAGAACCTTT
CACAGTCAACCCTGCCTTGACTCCAGCCAAGGACAAGAAAAAGAAAGACA
AAAAAAAGAAGGAATCTTCAAAGGAACTTGAAAGTCCTCTGACCCCTGGG
AAGGTGTGTCGAGCAGAGGAAGGCAAAAGCCCATTCAGGGAATCTTCAGG
AGATGGGATGAAAATGGAGGGGCTCCTAAATGGCTCATCAGACCCCCACC
AAAGCCGACTGGCTAGCATCAAGGCTGAAGCCGACAAGATCTACAGTTTC
ACGGACAATGCCCCCAGCCCTTCCATTGGAGGCAGTAGCCGCCTTGAAAA
CACTACCCCTACTCAGCCCCTGACTCCCTTACATGTGGTGACCCAGAATG
GAGCTGAAGCCAGCTCAGTCAAAACCAACAGCCCTGCATACTCTGACATC
TCTGATGCTGGGGAGGATGGGGAGGGCAAGGTAGACAGTGTCAAATCAAA
GGACGCCGAACAGTTGGTTAAAGAAGGGGCTAAGAAAACTCTTTTTCCCC
CTCAGCCTCAGAGCAAAGACTCACCATATTACCAAGGCTTTGAGAGTTAC
TATTCTCCAAGTTATGCACAGTCCAGCCCTGGGGCTCTGAACCCCAGCAG
CCAGGCAGGAGTGGAGAGCCAGGCCCTGAAGACAAAAAGGGATGAGGAAC
CTGAGAGCATAGAAGGGAAAGTGAAGAACGATATCTGTGAAGAAAAGAAG
CCCGAGCTGAGCAGTTCCAGTCAGCAGCCCTCGGTCATCCAGCAGCGTCC
CAATATGTACATGCAGTCCCTGTACTACAACCAGTATGCCTATGTACCCC
CCTATGGCTACAGCGACCAGAGTTACCACACCCACCTTCTGAGCACTAAC
ACGGCTTACCGGCAGCAGTACGAAGAACAGCAGAAACGCCAGAGCTTAGA
GCAGCAGCAGCGGGGAGTGGACAAGAAGGCAGAGATGGGCCTGAAGGAGC
GGGAGGCAGCACTCAAGGAAGAGTGGAAGCAAAAGCCGTCAATTCCACCA
ACTCTCACCAAGGCCCCCAGCCTGACAGACCTGGTGAAATCAGGACCTGG
CAAGGCCAAGGAGCCAGGGGCTGACCCAGCCAAATCAGTCATCATTCCCA
AGTTAGATGACTCTTCAAAACTCCCGGGCCAGGCCCCTGAAGGCCTTAAA
GTGAAGCTGAGTGATGCCAGCCACCTAAGCAAGGAGGCCTCTGAGGCCAA
GACAGGTGCTGAGTGTGGTCGACAGGCAGAGATGGATCCAATACTCTGGT
ACCGACAGGAGGCAGAGCCCCGGATGTGGACATATGTTTATCCTGCCAAG
TACTCAGACATCAAGTCAGAGGATGAGCGGTGGAAGGAGGAGCGGGACCG
CAAATTGAAGGAGGAAAGGAGTCGGAGTAAGGACTCTGTCCCCAAGGAAG
ATGGGAAGGAAAGCACAAGTAGTGACTGCAAGCTGCCCACGTCAGAGGAG
TCTCGCCTTGGGAGCAAGGAGCCCCGGCCAAGTGTCCATGTGCCTGTGTC
CTCCCCACTTACCCAGCACCAGTCCTACATCCCCTACATGCACGGCTATT
CCTACAGTCAGTCCTACGACCCCAACCACCCCAGCTACCGGAGCATGCCT
GCTGTGATGATGCAGAACTACCCAGGTTCCTACCTGCCTTCCAGCTACTC
TTTTTCCCCATATGGCAGCAAGGTCTCAGGTGGTGAAGATGCTGACAAGG
CACGAGCCAGCCCCAGTGTGACTTGTAAATCCAGCTCAGAGTCCAAAGCC
CTGGACATCTTGCAGCAGCATGCCAGTCACTACAAGAGCAAGTCTCCCAC
GATAAGTGATAAAACTTCTCAGGAGAGAGATCGAGGAGGCTGTGGGGTGG
TTGGGGGTGGTGGCAGCTGTAGCAGCGTCGGGGGAGCAAGTGGGGGTGAA
CGGAGTGTTGACCGGCCCCGCACCTCTCCTTCCCAGCGCCTGATGTCCAC
ACACCACCACCACCACCACTTGGGGTACTCATTGCTCCCAGCACAGTACA
ACTTACCCTATGCAGCAGGGCTTTCTTCTACAGCCATTGTTGCCAGCCAA
CAAGGCTCAACTCCCTCACTCTACCCACCCCCCAGGAGGTGAgcggccgc

Amino Acid Sequence

MYPYDVPDYAGSPGEFASLEMSLSSGASGGKGVDANPVETYDSGDEWDIG
VGNLIIDLDADLEKDQQKLEMSGSKEVGIPAPNAVATLPDNIKFVTPVPG
PQGKEGKSKSKRSKSGKDTSKPTPGTSLFTPSEGAASKKEVQGRSGDGAN
AGGLVAAIAPKGSEKAAKASRSVAGSKKEKENSSSKSKKERSEGVGTCSE
KDPGVLQPVPLGGRGGQYDGSAGVDTGAVEPLGSIAIEPGAALNPLGTKP
EPEEGENECRLLKKVKSEKMESPVSTPAVLPIHLLVPVVNNDISSPCEQI
MVRTRSVGVNTCDVALATEPECLGPCEPGTSVNLEGIVWQETEDGMLVVN
VTWRNKTYVGTLLDCTRHDWAPPRFCDSPTSDLEMRNGRGRGKRMRPNSN
TPVNETATASDSKGTSNSSKTRAGANSKGRRGSQNSSEHRPPASSTSEDV
KASPSSANKRKNKPLSDMELNSSSEDSKGSKRVRTNSMGSATGPLPGTKV
EPTVLDRNCPSPVLIDCPHPNCNKKYKHINGLKYHQAHAHTDDDSKPEAD
GDSEYGEEPILHADLGSCNGASVSQKGSLSPARSATPKVRLVEPHSPSPS
SKFSTKGLCKKKLSGEGDTDLGALSNDGSDDGPSVMDETSNDAFDSLERK
CMEKEKCKKPSSLKPEKIPSKSLKSARPIAPAIPPQQIYTFQTATFTAAS
PGSSSGLTATVAQAMPNSPQLKPIQPKPTVMGEPFTVNPALTPAKDKKKK
DKKKKESSKELESPLTPGKVCRAEEGKSPFRESSGDGMKMEGLLNGSSDP
HQSRLASIKAEADKIYSFTDNAPSPSIGGSSRLENTTPTQPLTPLHVVTQ
NGAEASSVKTNSPAYSDISDAGEDGEGKVDSVKSKDAEQLVKEGAKKTLF
PPQPQSKDSPYYQGFESYYSPSYAQSSPGALNPSSQAGVESQALKTKRDE
EPESIEGKVKNDICEEKKPELSSSSQQPSVIQQRPNMYMQSLYYNQYAYV
PPYGYSDQSYHTHLLSTNTAYRQQYEEQQKRQSLEQQQRGVDKKAEMGLK
EREAALKEEWKQKPSIPPTLTKAPSLTDLVKSGPGKAKEPGADPAKSVII
PKLDDSSKLPGQAPEGLKVKLSDASHLSKEASEAKTGAECGRQAEMDPIL
WYRQEAEPRMWTYVYPAKYSDIKSEDERWKEERDRKLKEERSRSKDSVPK
EDGKESTSSDCKLPTSEESRLGSKEPRPSVHVPVSSPLTQHQSYIPYMHG
YSYSQSYDPNHPSYRSMPAVMMQNYPGSYLPSSYSFSPYGSKVSGGEDAD
KARASPSVTCKSSSESKALDILQQHASHYKSKSPTISDKTSQERDRGGCG
VVGGGGSCSSVGGASGGERSVDRPRTSPSQRLMSTHHHHHHLGYSLLPAQ
YNLPYAAGLSSTAIVASQQGSTPSLYPPPRR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65456
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 41456; Silent change at Pro276 (CCC > CCT) compared with NCBI accession
• Low Temp: No