cDNA Clone SETDB1

• Plasmid: pcDNA5D-FRT/TO HA-SETDB1
• Parent Plasmid: pcDNA5D-FRT/TO HA
• Expressed: HA-SETDB1
• Genbank: NM_001145415.2 → NP_001138887.1 AL590133 (Uniprot Q15047)
• Tag: HA
• Species: Human

Sequence of Insert

ctcgacATGTCTTCCCTTCCTGGGTGCATTGGTTTGGATGCAGCAACAGC
TACAGTGGAGTCTGAAGAGATTGCAGAGCTGCAACAGGCAGTGGTTGAGG
AACTGGGTATCTCTATGGAGGAACTTCGGCATTTCATCGATGAGGAACTG
GAGAAGATGGATTGTGTACAGCAACGCAAGAAGCAGCTAGCAGAGTTAGA
GACATGGGTAATACAGAAAGAATCTGAGGTGGCTCACGTTGACCAACTCT
TTGATGATGCATCCAGGGCAGTGACTAATTGTGAGTCTTTGGTGAAGGAC
TTCTACTCCAAGCTGGGACTACAATACCGGGACAGTAGCTCTGAGGACGA
ATCTTCCCGGCCTACAGAAATAATTGAGATTCCTGATGAAGATGATGATG
TCCTCAGTATTGATTCAGGTGATGCTGGGAGCAGAACTCCAAAAGACCAG
AAGCTCCGTGAAGCTATGGCTGCCTTAAGAAAGTCAGCTCAAGATGTTCA
GAAGTTCATGGATGCTGTCAACAAGAAGAGCAGTTCCCAGGATCTGCATA
AAGGAACCTTGAGTCAGATGTCTGGAGAACTAAGCAAAGATGGTGACCTG
ATAGTCAGCATGCGAATTCTGGGCAAGAAGAGAACTAAGACTTGGCACAA
AGGCACCCTTATTGCCATCCAGACAGTTGGGCCAGGGAAGAAATACAAGG
TGAAATTTGACAACAAAGGAAAGAGTCTACTGTCGGGGAACCATATTGCC
TATGATTACCACCCTCCTGCTGACAAGCTGTATGTGGGCAGTCGGGTGGT
CGCCAAATACAAAGATGGGAATCAGGTCTGGCTCTATGCTGGCATTGTAG
CTGAGACACCAAACGTCAAAAACAAGCTCAGGTTTCTCATTTTCTTTGAT
GATGGCTATGCTTCCTATGTCACACAGTCGGAACTGTATCCCATTTGCCG
GCCACTGAAAAAGACTTGGGAGGACATAGAAGACATCTCCTGCCGTGACT
TCATAGAGGAGTATGTCACTGCCTACCCCAACCGCCCCATGGTACTGCTC
AAGAGTGGCCAGCTTATCAAGACTGAGTGGGAAGGCACGTGGTGGAAGTC
CCGAGTTGAGGAGGTGGATGGCAGCCTAGTCAGGATCCTCTTCCTGGATG
ACAAAAGATGTGAGTGGATCTATCGAGGCTCTACACGGCTGGAGCCCATG
TTCAGCATGAAAACATCCTCAGCCTCTGCACTGGAGAAGAAGCAAGGACA
GCTCAGGACACGTCCAAATATGGGTGCTGTGAGGAGCAAAGGCCCTGTTG
TCCAGTACACACAGGATCTGACCGGTACTGGAACCCAGTTCAAGCCAGTG
GAACCCCCACAGCCTACAGCTCCACCTGCCCCACCTTTCCCACCTGCTCC
ACCTCTATCCCCCCAAGCAGGTGACAGTgaaagcTTGGAAAGCCAGCTTG
CCCAGTCACGGAAGCAGGTAGCCAAAAAGAGCACGTCCTTTCGACCAGGA
TCTGTGGGCTCTGGTCATTCCTCCCCTACATCTCCTGCACTCAGTGAAAA
TGTCTCTGGTGGGAAACCTGGGATCAACCAGACATATAGATCACCTTTAG
GCTCCACAGCCTCTGCCCCAGCACCCTCAGCACTCCCGGCCCCTCCAGCA
CCCCCAGTCTTCCATGGCATGCTGGAGCGGGCCCCAGCAGAGCCCTCCTA
CCGTGCTCCCATGGAGAAGCTTTTCTACTTACCTCATGTCTGCAGCTATA
CCTGTCTGTCTCGAGTCAGACCTATGAGGAATGAGCAGTACCGGGGCAAG
AACCCTCTGCTGGTCCCGTTACTATATGACTTCCGGCGGATGACAGCCCG
GCGTCGAGTTAACCGCAAGATGGGCTTTCATGTTATCTATAAGACACCTT
GTGGTCTCTGCCTTCGGACAATGCAGGAGATAGAACGCTACCTTTTCGAG
ACTGGCTGTGACTTCCTCTTCCTGGAGATGTTCTGTTTGGATCCATATGT
TCTTGTGGACCGAAAGTTTCAGCCCTATAAGCCTTTTTACTATATTTTGG
ACATCACTTATGGGAAGGAAGATGTTCCCCTATCCTGTGTCAATGAGATT
GACACAACCCCTCCACCCCAGGTGGCCTACAGCAAGGAACGTATCCCGGG
CAAGGGTGTTTTCATTAACACAGGCCCTGAATTTCTGGTTGGCTGTGACT
GCAAGGATGGGTGTCGGGACAAGTCCAAGTGTGCCTGCCATCAACTAACT
ATCCAGGCTACAGCCTGTACCCCAGGAGGCCAAATCAACCCTAACTCTGG
CTACCAGTACAAGAGACTAGAAGAGTGTCTACCCACAGGGGTATATGAGT
GTAACAAACGCTGCAAATGTGACCCAAACATGTGCACAAACCGGTTGGTG
CAACATGGACTACAAGTTCGGCTACAGCTATTCAAGACACAGAACAAGGG
CTGGGGTATCCGCTGCTTGGATGACATTGCCAAAGGCTCTTTTGTTTGTA
TTTATGCAGGCAAAATCCTGACAGATGACTTTGCAGACAAGGAGGGTCTG
GAAATGGGTGATGAGTACTTTGCAAATCTGGACCATATCGAGAGCGTGGA
GAACTTCAAAGAAGGATATGAGAGTGATGCCCCCTGTTCCTCTGACAGCA
GTGGTGTAGACTTGAAGGACCAGGAAGATGGCAACAGCGGTACAGAGGAC
CCTGAAGAGTCCAATGATGATAGCTCAGATGATAACTTCTGTAAGGATGA
GGACTTCAGCACCAGTTCAGTGTGGCGGAGCTATGCTACCCGGAGGCAGA
CCCGGGGCCAGAAAGAGAACGGACTCTCTGAGACAACTTCCAAGGACTCC
CACCCCCCAGATCTTGGACCCCCACATATTCCTGTTCCTCCCTCAATCCC
TGTAGGTGGCTGCAATCCACCTTCCTCCGAAGAGACACCCAAGAACAAGG
TGGCCTCATGGTTGAGCTGCAATAGTGTCAGTGAAGGTGGTTTTGCTGAC
TCTGATAGCCATTCATCCTTCAAGACTAATGAAGGTGGGGAGGGCCGGGC
TGGGGGAAGCCGAATGGAGGCTGAGAAGGCCTCCACCTCAGGACTAGGCA
TCAAGGATGAGGGAGACATCAAACAGGCCAAGAAAGAGGACACTGACGAC
CGAAACAAGATGTCAGTAGTTACTGAAAGCTCTCGAAATTACGGTTACAA
TCCTTCTCCTGTGAAGCCTGAAGGACTTCGCCGCCCACCTAGTAAGACTA
GTATGCATCAAAGCCGAAGACTCATGGCTTCTGCTCAGTCCAACCCTGAT
GATGTCCTGACACTGTCCAGCAGCACAGAAAGTGAGGGGGAAAGTGGGAC
CAGCCGAAAGCCCACTGCTGGTCAGACTTCGGCTACAGCGGTTGACAGTG
ATGATATCCAGACCATATCCTCTGGCTCTGAAGGGGATGACTTTGAGGAC
AAGAAGAACATGACTGGTCCAATGAAGCGTCAAGTGGCAGTAAAATCAAC
CCGAGGCTTTGCTCTTAAATCAACCCATGGGATTGCAATTAAATCAACCA
ACATGGCCTCTGTGGACAAGGGGGAGAGCGCACCTGTTCGTAAGAACACA
CGCCAATTCTATGATGGCGAGGAGTCTTGCTACATCATTGATGCCAAGCT
TGAAGGCAACCTGGGCCGCTACCTCAACCACAGTTGCAGCCCCAACCTGT
TTGTCCAGAATGTCTTCGTGGATACCCATGATCTTCGCTTCCCCTGGGTG
GCCTTCTTTGCCAGCAAAAGAATCCGGGCTGGGACAGAACTTACTTGGGA
CTACAACTACGAGGTGGGCAGTGTGGAAGGCAAGGAGCTACTCTGTTGCT
GTGGGGCCATTGAATGCAGAGGACGTCTTCTTTAGgcggccgc

Amino Acid Sequence

MYPYDVPDYAGSPGEFASLDMSSLPGCIGLDAATATVESEEIAELQQAVV
EELGISMEELRHFIDEELEKMDCVQQRKKQLAELETWVIQKESEVAHVDQ
LFDDASRAVTNCESLVKDFYSKLGLQYRDSSSEDESSRPTEIIEIPDEDD
DVLSIDSGDAGSRTPKDQKLREAMAALRKSAQDVQKFMDAVNKKSSSQDL
HKGTLSQMSGELSKDGDLIVSMRILGKKRTKTWHKGTLIAIQTVGPGKKY
KVKFDNKGKSLLSGNHIAYDYHPPADKLYVGSRVVAKYKDGNQVWLYAGI
VAETPNVKNKLRFLIFFDDGYASYVTQSELYPICRPLKKTWEDIEDISCR
DFIEEYVTAYPNRPMVLLKSGQLIKTEWEGTWWKSRVEEVDGSLVRILFL
DDKRCEWIYRGSTRLEPMFSMKTSSASALEKKQGQLRTRPNMGAVRSKGP
VVQYTQDLTGTGTQFKPVEPPQPTAPPAPPFPPAPPLSPQAGDSESLESQ
LAQSRKQVAKKSTSFRPGSVGSGHSSPTSPALSENVSGGKPGINQTYRSP
LGSTASAPAPSALPAPPAPPVFHGMLERAPAEPSYRAPMEKLFYLPHVCS
YTCLSRVRPMRNEQYRGKNPLLVPLLYDFRRMTARRRVNRKMGFHVIYKT
PCGLCLRTMQEIERYLFETGCDFLFLEMFCLDPYVLVDRKFQPYKPFYYI
LDITYGKEDVPLSCVNEIDTTPPPQVAYSKERIPGKGVFINTGPEFLVGC
DCKDGCRDKSKCACHQLTIQATACTPGGQINPNSGYQYKRLEECLPTGVY
ECNKRCKCDPNMCTNRLVQHGLQVRLQLFKTQNKGWGIRCLDDIAKGSFV
CIYAGKILTDDFADKEGLEMGDEYFANLDHIESVENFKEGYESDAPCSSD
SSGVDLKDQEDGNSGTEDPEESNDDSSDDNFCKDEDFSTSSVWRSYATRR
QTRGQKENGLSETTSKDSHPPDLGPPHIPVPPSIPVGGCNPPSSEETPKN
KVASWLSCNSVSEGGFADSDSHSSFKTNEGGEGRAGGSRMEAEKASTSGL
GIKDEGDIKQAKKEDTDDRNKMSVVTESSRNYGYNPSPVKPEGLRRPPSK
TSMHQSRRLMASAQSNPDDVLTLSSSTESEGESGTSRKPTAGQTSATAVD
SDDIQTISSGSEGDDFEDKKNMTGPMKRQVAVKSTRGFALKSTHGIAIKS
TNMASVDKGESAPVRKNTRQFYDGEESCYIIDAKLEGNLGRYLNHSCSPN
LFVQNVFVDTHDLRFPWVAFFASKRIRAGTELTWDYNYEVGSVEGKELLC
CCGAIECRGRLL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65455
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 41456; Isoform 1. Differs from NCBI accession at D475E and insertion of extra residue Ser476
• Low Temp: No