cDNA Clone UFM1

• Synonyms: C13orf20, HLD14
• Plasmid: pTXB1-UFM1 (MGCSSGAS-Met1-GCSSG-Ser2 - Asp80) T3C Mxe intein CBD
• Parent Plasmid: pTXB1
• Expressed: UFM1 (MGCSSGAS-Met1-GCSSG-Ser2-Asp80) T3C Mxe intein CBD
• Genbank: NM_016617.4 → NP_057701.1
• Tag: Intein
• Species: Human

Amino Acid Sequence

MGCSSGASMGCSSGSKVSFKITLTSDPRLPYKVLSVPESTPFTAVLKFAA
EEFKVPAATSAIITNDGIGINPAQTAGNVFLKHGSELRIIPRDCICGDAL
VALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPVLADRLFHSGEHP
VYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAVIQR
SAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELT
DGRFYYAKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHATGLTGLNSG
LTTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNVPALW
QLQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU65446
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: additional N-terminal residues (MAS) derived from translation initiation at the pTXB1 vector derived Nde1 site. GCSSG now inserted between this Met1 and Ala2. Native UFM1 Met1 = Met9 of translation product (with GCSSG also between Met1 and Ser2 of UFM1 ORF - deriving from 29487); Intein T3C mutant; ; PARENTAL 45876
• Low Temp: No