cDNA Clone ATXN3

• Plasmid: pGEX6P-1-ATXN3 F74A
• Parent Plasmid: pGEX6P1
• Expressed: GST ATXN3 F74A
• Genbank: AAH33711.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGAGTCCATCTTCCACGAGAAACAAGAAGGCTCACTTTGTGC
TCAACATTGCCTGAATAACTTATTGCAAGGAGAATATTTTAGCCCTGTGG
AATTATCCTCAATTGCACATCAGCTGGATGAGGAGGAGAGGATGAGAATG
GCAGAAGGAGGAGTTACTAGTGAAGATTATCGCACGTTTTTACAGCAGCC
TTCTGGAAATATGGATGACAGTGGTgcTTTCTCTATTCAGGTTATAAGCA
ATGCCTTGAAAGTTTGGGGTTTAGAACTAATCCTGTTCAACAGTCCAGAG
TATCAGAGGCTCAGGATCGATCCTATAAATGAAAGATCATTTATATGCAA
TTATAAGGAACACTGGTTTACAGTTAGAAAATTAGGAAAACAGTGGTTTA
ACTTGAATTCTCTCTTGACGGGTCCAGAATTAATATCAGATACATATCTT
GCACTTTTCTTGGCTCAATTACAACAGGAAGGTTATTCTATATTTGTTGT
TAAGGGTGATCTGCCAGATTGCGAAGCTGACCAACTCCTGCAGATGATTA
GGGTCCAACAGATGCATCGACCAAAACTTATTGGAGAAGAATTAGCACAA
CTAAAAGAGCAAAGAGTCCATAAAACAGACCTGGAACGAGTGTTAGAAGC
AAATGATGGCTCAGGAATGTTAGACGAAGATGAGGAGGATTTGCAGAGGG
CTCTGGCACTAAGTCGCCAAGAAATTGACATGGAAGATGAGGAAGCAGAT
CTCCGCAGGGCTATTCAGCTAAGTATGCAAGGTAGTTCCAGAAACATATC
TCAAGATATGACACAGACATCAGGTACAAATCTTACTTCAGAAGAGCTTC
GGAAGAGACGAGAAGCCTACTTTGAAAAACAGCAGCAAAAGCAGCAACAG
CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGGG
GGACCTATCAGGACAGAGTTCACATCCATGTGAAAGGCCAGCCACCAGTT
CAGGAGCACTTGGGAGTGATCTAGGTGATGCTATGAGTGAAGAAGACATG
CTTCAGGCAGCTGTGACCATGTCTTTAGAAACTGTCAGAAATGATTTGAA
AACAGAAGGAAAAAAATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMESIFHEKQEGSLCAQHCL
NNLLQGEYFSPVELSSIAHQLDEEERMRMAEGGVTSEDYRTFLQQPSGNM
DDSGAFSIQVISNALKVWGLELILFNSPEYQRLRIDPINERSFICNYKEH
WFTVRKLGKQWFNLNSLLTGPELISDTYLALFLAQLQQEGYSIFVVKGDL
PDCEADQLLQMIRVQQMHRPKLIGEELAQLKEQRVHKTDLERVLEANDGS
GMLDEDEEDLQRALALSRQEIDMEDEEADLRRAIQLSMQGSSRNISQDMT
QTSGTNLTSEELRKRREAYFEKQQQKQQQQQQQQQQQQQQQQQQQGDLSG
QSSHPCERPATSSGALGSDLGDAMSEEDMLQAAVTMSLETVRNDLKTEGK
K*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU65487
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: silent change at Val164 (GTC > GTT)compared to NCBI ref
• Low Temp: No