cDNA Clone TIFA

• Plasmid: pCMV5 FLAG1 TIFA E178A
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG-TIFA E178A
• Genbank: NM_052864.3 → NP_443096.1
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCACCAGTTTTGAAGATGCTGACACAGAAGAGACAGTAACTTGTCTCCAGATGACGGTTTACCATCCTGGCCAGTTGCAGTGTGGAATATTTCAGTCAATAAGTTTTAACAGAGAGAAACTCCCTTCCAGCGAAGTGGTGAAATTTGGCCGAAATTCCAACATCTGTCATTATACTTTTCAGGACAAACAGGTTTCCCGAGTTCAGTTTTCTCTGCAGCTGTTTAAAAAATTCAACAGCTCAGTTCTCTCCTTTGAAATAAAAAATATGAGTAAAAAGACCAATCTGATCGTGGACAGCAGAGAGCTGGGCTACCTAAATAAAATGGACCTGCCATACAGGTGCATGGTCAGATTCGGAGAGTATCAGTTTCTGATGGAGAAGGAAGATGGCGAGTCATTGGAATTTTTTGAGACTCAATTTATTTTATCTCCAAGATCACTCTTGCAAGAAAACAACTGGCCACCACACAGGCCCATACCGGAGTATGGCACTTACTCGCTCTGCTCCTCCCAAAGCAGTTCTCCGACAGCAATGGATGAAAATGAGTCATGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSTSFEDADTEETVTCLQMTVYHPGQLQCGIFQSISFNREK
LPSSEVVKFGRNSNICHYTFQDKQVSRVQFSLQLFKKFNSSVLSFEIKNM
SKKTNLIVDSRELGYLNKMDLPYRCMVRFGEYQFLMEKEDGESLEFFETQ
FILSPRSLLQENNWPPHRPIPEYGTYSLCSSQSSSPTAMDENES*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65528
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Source 32329 Y166 (TAT > TAC) compared to NCBI ref. No native initiating methionine
• Low Temp: No