cDNA Clone UBE3A

• Plasmid: pGEX-TEV-UBE3A L770-L875 C843A
• Parent Plasmid: pGEX-TEV
• Expressed: GST-TEV-UBE3A (aa770-875) C843A
• Genbank: NM_000462.3 Q05086
• Tag: GST
• Species: Human

Sequence of Insert

ggatccCTAGAAGAAACTACAGAATATGACGGTGGCTATACCAGGGACTC
TGTTCTGATTAGGGAGTTCTGGGAAATCGTTCATTCATTTACAGATGAAC
AGAAAAGACTCTTCTTGCAGTTTACAACGGGCACAGACAGAGCACCTGTG
GGAGGACTAGGAAAATTAAAGATGATTATAGCCAAAAATGGCCCAGACAC
AGAAAGGTTACCTACATCTCATACTgcCTTTAATGTGCTTTTACTTCCGG
AATACTCAAGCAAAGAAAAACTTAAAGAGAGATTGTTGAAGGCCATCACG
TATGCCAAAGGATTTGGCATGCTGTAAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDENLYFQGGSLEETTEYDGGYTRDSVLIREF
WEIVHSFTDEQKRLFLQFTTGTDRAPVGGLGKLKMIIAKNGPDTERLPTS
HTAFNVLLLPEYSSKEKLKERLLKAITYAKGFGML*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU65503
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 20757; Transcript variant 2
• Low Temp: No