cDNA Clone UBE2S

• Synonyms: E2-EPF; E2EPF; EPF5
• Plasmid: pET156P-1 UBE2S C95A/K100A
• Parent Plasmid: pET156P-1
• Expressed: 6His UBE2S C95A/K100A
• Genbank: NM_014501.3
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCAACTCCAACGTGGAGAACCTACCCCCGCACATCATCCGCCTGGTGTACAAGGAGGTGACGACACTGACCGCAGACCCACCCGATGGCATCAAGGTCTTTCCCAACGAGGAGGACCTCACCGACCTCCAGGTCACCATCGAGGGCCCTGAGGGGACCCCATATGCTGGAGGTCTGTTCCGCATGAAACTCCTGCTGGGGAAGGACTTCCCTGCCTCCCCACCCAAGGGCTACTTCCTGACCAAGATCTTCCACCCGAACGTGGGCGCCAATGGCGAGATCGCCGTCAACGTGCTCGCGAGGGACTGGACGGCTGAGCTGGGCATCCGACACGTACTGCTGACCATCAAGTGCCTGCTGATCCACCCTAACCCCGAGTCTGCACTCAACGAGGAGGCGGGCCGCCTGCTCTTGGAGAACTACGAGGAGTATGCAGCTCGGGCCCGTCTGCTCACAGAGATCCACGGGGGCGCCGGCGGGCCCAGCGGCAGGGCCGAAGCCGGTCGGGCCCTGGCCAGTGGCACTGAAGCTTCCTCCACCGACCCTGGGGCCCCAGGGGGCCCGGGAGGGGCTGAGGGTCCCATGGCCAAGAAGCATGCTGGCGAGCGCGATAAGAAGCTGGCGGCCAAGAAAAAGACGGACAAGAAGCGGGCGCTGCGGCGGCTGTAGGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSNSNVENLPPHIIRLVYKEVTTLTADPP
DGIKVFPNEEDLTDLQVTIEGPEGTPYAGGLFRMKLLLGKDFPASPPKGY
FLTKIFHPNVGANGEIAVNVLARDWTAELGIRHVLLTIKCLLIHPNPESA
LNEEAGRLLLENYEEYAARARLLTEIHGGAGGPSGRAEAGRALASGTEAS
STDPGAPGGPGGAEGPMAKKHAGERDKKLAAKKKTDKKRALRRL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU65524
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent change at Ala145 (GCG > GCA); UBE2S has no native initiating methionine
• Low Temp: No